pLV3-CMV-Lrrc15-3×FLAG-CopGFP-

pLV3-CMV-Lrrc15-3×FLAG-CopGFP-Blast plasmid

PVT96456

pLV3-CMV-Lrrc15-3×FLAG-CopGFP-Blast plasmid Information

Plasmid name: pLV3-CMV-Lrrc15(mouse)-3×FLAG-CopGFP-Blast
Alias: NM_028973.2;5430427N11Rik; LIB
Gene lenght: 1737bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Blast
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-Lrrc15-3×FLAG-CopGFP-Blast plasmid Description

pLV3-CMV-Lrrc15-3×FLAG-CopGFP-Blast It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-Lrrc15-3×FLAG-CopGFP-Blast plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgc cgctgaaaca ttatctcctt 2341 ttgctggtga gctgccaggc ctgggctgca ggtttggcct actatggctg ccccagcgaa 2401 tgtacctgtt cccgggcctc ccaggtagag tgcacagggg cacaaattgt ggcgatgcct 2461 tcccctctgc cctggaatgc tatgagcctg cagatcctta acacacacat caccgaactc 2521 cccgaggaca agttcctcaa catttcagcc ctcatcgccc tgaagatgga gaagaacgag 2581 ctggccaaca tcatgccagg tgccttccgc aatctgggct cgctgcgtca cctcagcctt 2641 gccaacaaca aactaaagaa cctgcccgtt aggctcttcc aggatgtgaa caatctggag 2701 accctcctgc tgtccaataa ccagctggtg cagatccagc cggcccagtt ctcccagttt 2761 agtaatctta aggaactcca gttgtatggc aacaatctgg aatacatccc cgaaggagtc 2821 tttgaccacc tggtaggtct caccaaactc aacctgggca acaacggctt cacccaccta 2881 tcccctaggg tctttcagca tctgggcaac ctccaggtgc ttcggctata tgagaacagg 2941 ctttcagaca tccccatggg cacttttgat gcacttggca acctccagga gctggccctc 3001 caagagaacc agattggcac cctctcccct ggcttgttcc acaataaccg taacctccag 3061 agactgtatc tatccaacaa ccacatctca catctgcccc ctggcatctt catgcagctg 3121 ccccacctta acaagctcac actctttggg aattccctga aggagctttc cccgggggtc 3181 tttgggccca tgcccaacct gagggagctt tggctctaca acaatcacat tacctccctc 3241 cctgacaatg ccttcagcca cctgaaccag ttgcaggttc tgattcttag tcacaaccag 3301 ctcagttaca tctccccagg agccttcaat gggctgacga atctccgaga actgtccctc 3361 cacaccaatg ctctacagga cctggatggg aatgtcttcc gatcactggc caacctgagg 3421 aacgtctcac tccagaataa ccgccttcga cagctccctg gcagcatctt cgccaacgtc 3481 aatggtctca tgaccatcca actgcagaac aacaatctag agaacttgcc tctgggcatc 3541 tttgatcact tagggaacct gtgtgagctt cgtctctatg acaacccctg gagatgtgac 3601 tcaaacatcc tcccacttca tgactggctc attctcaaca gagccaggtt ggggacagac 3661 actcttccag tgtgttccag tccagccagt gtccgaggcc agtcccttgt catcatcaat 3721 gtcaatttcc ctggacccag tgtccagggc ccagaaaccc ctgaagtatc tagctaccca 3781 gatacatcca gctatcctga cagcacatcc atttcttcta ccacagagat caccaggtct 3841 accgatgatg actacaccga tctaaacacc atcgaaccca ttgatgaccg caatacatgg 3901 ggcatgactg atgcccagag tgggctggcc attgctgcca ttgtgattgg catcatagct 3961 ttggcctgtt ccctcgctgc ctgtatctgc tgctgctgct gcaaaaagag gagccaggca 4021 gtcctgatgc agatgaaggc tcccaatgag tgcGACTACA AAGACCATGA CGGTGATTAT 4081 AAAGATCATG ACATCGACTA CAAGGATGAC GATGACAAGT AGggatccCT GCGATATCCA 4141 GCgcggccgc Gaaggatctg cgatcgctcc ggtgcccgtc agtgggcaga gcgcacatcg 4201 cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaacgggtgc ctagagaagg 4261 tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt 4321 gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt 4381 gccgccagaa cacagctgaa gcttcgaggg gctcgcatct ctccttcacg cgcccgccgc 4441 cctacctgag gccgccatcc acgccggttg agtcgcgttc tgccgcctcc cgcctgtggt 4501 gcctcctgaa ctgcgtccgc cgtctaggta agtttaaagc tcaggtcgag accgggcctt 4561 tgtccggcgc tcccttggag cctacctaga ctcagccggc tctccacgct ttgcctgacc 4621 ctgcttgctc aactctacgt ctttgtttcg ttttctgttc tgcgccgtta cagatccaag 4681 ctgtgaccgg cgcctacgct agacgccacc atggagagcg acgagagcgg cctgcccgcc 4741 atggagatcg agtgccgcat caccggcacc ctgaacggcg tggagttcga gctggtgggc 4801 ggcggagagg gcacccccaa gcagggccgc atgaccaaca agatgaagag caccaaaggc 4861 gccctgacct tcagccccta cctgctgagc cacgtgatgg gctacggctt ctaccacttc 4921 ggcacctacc ccagcggcta cgagaacccc ttcctgcacg ccatcaacaa cggcggctac 4981 accaacaccc gcatcgagaa gtacgaggac ggcggcgtgc tgcacgtgag cttcagctac 5041 cgctacgagg ccggccgcgt gatcggcgac ttcaaggtgg tgggcaccgg cttccccgag 5101 gacagcgtga tcttcaccga caagatcatc cgcagcaacg ccaccgtgga gcacctgcac 5161 cccatgggcg ataacgtgct ggtgggcagc ttcgcccgca ccttcagcct gcgcgacggc 5221 ggctactaca gcttcgtggt ggacagccac atgcacttca agagcgccat ccaccccagc 5281 atcctgcaga acgggggccc catgttcgcc ttccgccgcg tggaggagct gcacagcaac 5341 accgagctgg gcatcgtgga gtaccagcac gccttcaaga cccccatcgc cttcgccaga 5401 tcccgcgctc agtcgtccaa ttctgccgtg gacggcaccg ccggacccgg ctccaccgga 5461 tctcgcgagg gcagaggaag tcttctaaca tgcggtgacg tggaggagaa tcccggccct 5521 ATGGCCAAGC CTTTGTCTCA AGAAGAATCC ACCCTCATTG AAAGAGCAAC GGCTACAATC 5581 AACAGCATCC CCATCTCTGA AGACTACAGC GTCGCCAGCG CAGCTCTCTC TAGCGACGGC 5641 CGCATCTTCA CTGGTGTCAA TGTATATCAT TTTACTGGGG GACCTTGTGC AGAACTCGTG 5701 GTGCTGGGCA CTGCTGCTGC TGCGGCAGCT GGCAACCTGA CTTGTATCGT CGCGATCGGA 5761 AATGAGAACA GGGGCATCTT GAGCCCCTGC GGACGGTGCC GACAGGTGCT TCTCGATCTG 5821 CATCCTGGGA TCAAAGCCAT AGTGAAGGAC AGTGATGGAC AGCCGACGGC AGTTGGGATT 5881 CGTGAATTGC TGCCCTCTGG TTATGTGTGG GAGGGCTAAG TCGACAATCA ACCTCTGGAT 5941 TACAAAATTT GTGAAAGATT GACTGGTATT CTTAACTATG TTGCTCCTTT TACGCTATGT 6001 GGATACGCTG CTTTAATGCC TTTGTATCAT GCTATTGCTT CCCGTATGGC TTTCATTTTC 6061 TCCTCCTTGT ATAAATCCTG GTTGCTGTCT CTTTATGAGG AGTTGTGGCC CGTTGTCAGG 6121 CAACGTGGCG TGGTGTGCAC TGTGTTTGCT GACGCAACCC CCACTGGTTG GGGCATTGCC 6181 ACCACCTGTC AGCTCCTTTC CGGGACTTTC GCTTTCCCCC TCCCTATTGC CACGGCGGAA 6241 CTCATCGCCG CCTGCCTTGC CCGCTGCTGG ACAGGGGCTC GGCTGTTGGG CACTGACAAT 6301 TCCGTGGTGT TGTCGGGGAA ATCATCGTCC TTTCCTTGGC TGCTCGCCTG TGTTGCCACC 6361 TGGATTCTGC GCGGGACGTC CTTCTGCTAC GTCCCTTCGG CCCTCAATCC AGCGGACCTT 6421 CCTTCCCGCG GCCTGCTGCC GGCTCTGCGG CCTCTTCCGC GTCTTCGCCT TCGCCCTCAG 6481 ACGAGTCGGA TCTCCCTTTG GGCCGCCTCC CCGCctggta cctttaagac caatgactta 6541 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat 6601 tcactcccaa cgaaaataag atctgctttt tgcttgtact gggtctctct ggttagacca 6661 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag 6721 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag 6781 atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct 6841 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt 6901 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc 6961 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt 7021 ctggctctag ctatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc 7081 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag 7141 aagtagtgag gaggcttttt tggaggccta gacttttgca gagacggccc aaattcgtaa 7201 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata 7261 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta 7321 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa 7381 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg 7441 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag 7501 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa 7561 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc 7621 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca 7681 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg 7741 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct 7801 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt 7861 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag 7921 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc 7981 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac 8041 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga 8101 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc 8161 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg 8221 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca 8281 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt 8341 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca 8401 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg 8461 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca 8521 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt 8581 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt 8641 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca 8701 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca 8761 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga 8821 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact 8881 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga 8941 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg 9001 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc 9061 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga 9121 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat 9181 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt 9241 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt 9301 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac 9361 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc 9421 tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag 9481 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca 9541 gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg 9601 agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc 9661 aggcgccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct 9721 tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg 9781 ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgccaagct g

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.