pCMV-MCOLN3-GCaMP6f-HA-Neo pla

pCMV-MCOLN3-GCaMP6f-HA-Neo plasmid

PVT86689

pCMV-MCOLN3-GCaMP6f-HA-Neo plasmid Information

Plasmid name: pCMV-MCOLN3(human)-GCaMP6f-HA-Neo
Alias: NM_018298.11;TRP-ML3; TRPML3;
Gene lenght: 1659bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone: pCDNA3.1-HA-C
Forward primer:
Reverse primer:
Copy number: High
Induction:

pCMV-MCOLN3-GCaMP6f-HA-Neo plasmid Description

pCMV-MCOLN3-GCaMP6f-HA-Neo This plasmid is suitable for overexpression in mammalian cells. The plasmid itself.

pCMV-MCOLN3-GCaMP6f-HA-Neo plasmid Sequence

1 GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATCTG CTCCCTGCTT 61 GTGTGTTGGA GGTCGCTGAG TAGTGCGCGA GCAAAATTTA AGCTACAACA AGGCAAGGCT 121 TGACCGACAA TTGCATGAAG AATCTGCTTA GGGTTAGGCG TTTTGCGCTG CTTCGCGATG 181 TACGGGCCAG ATATACGCGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA 241 CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG 301 GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC 361 CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA 421 CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA 481 ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA 541 CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT 601 ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG 661 ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA 721 ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA 781 GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT AATACGACTC 841 ACTATAGGGA GACCCAAGCT GGCTAGCGTT TAAACTTAAG CTTGGTACCG AGCTCGGATC 901 CGCCACCATG GCAGATCCTG AGGTAGTTGT GAGTAGCTGC AGCTCTCATG AAGAGGAAAA 961 TCGCTGCAAT TTTAACCAGC AAACATCTCC ATCTGAGGAG CTTCTATTAG AAGACCAGAT 1021 GAGGCGAAAA CTCAAATTTT TTTTCATGAA TCCCTGTGAG AAGTTCTGGG CTCGAGGTAG 1081 AAAACCATGG AAACTTGCCA TACAAATTCT AAAAATTGCA ATGGTGACTA TCCAGCTGGT 1141 CTTATTTGGG CTAAGTAACC AGATGGTGGT AGCTTTCAAG GAAGAGAATA CTATAGCATT 1201 CAAACACCTT TTCCTAAAAG GATATATGGA CCGAATGGAT GACACATATG CAGTGTACAC 1261 ACAAAGTGAC GTGTATGATC AGTTAATCTT CGCAGTAAAC CAGTACTTGC AGCTATACAA 1321 TGTCTCCGTT GGGAATCATG CTTATGAGAA CAAAGGTACC AAGCAATCTG CTATGGCAAT 1381 CTGTCAGCAC TTCTACAAGC GAGGAAACAT CTACCCTGGA AATGATACCT TTGACATCGA 1441 TCCAGAAATT GAAACTGAGT GTTTCTTTGT GGAGCCAGAT GAACCTTTTC ACATTGGGAC 1501 ACCAGCAGAA AATAAACTGA ACTTAACACT GGACTTCCAC AGACTCCTAA CAGTGGAGCT 1561 TCAGTTTAAA CTGAAAGCCA TTAATCTGCA GACAGTTCGT CATCAAGAAC TCCCTGACTG 1621 TTATGACTTT ACTCTGACTA TAACATTTGA CAACAAGGCC CATAGTGGAA GAATTAAAAT 1681 AAGTTTAGAT AATGACATTT CCATCAGAGA ATGTAAAGAC TGGCATGTAT CTGGATCAAT 1741 TCAGAAGAAC ACTCATTACA TGATGATCTT TGATGCCTTT GTCATTCTGA CTTGCTTGGT 1801 TTCATTAATC CTCTGCATTA GATCTGTGAT TAGAGGACTT CAGCTTCAGC AGGAGTTTGT 1861 CAATTTTTTC CTCCTCCATT ATAAGAAGGA AGTTTCTGTT TCTGATCAAA TGGAATTTGT 1921 CAATGGATGG TACATTATGA TTATTATTAG TGACATATTG ACAATCATTG GATCAATTCT 1981 AAAAATGGAA ATCCAAGCTA AGAGTCTAAC TAGTTATGAT GTCTGTAGCA TACTTCTTGG 2041 GACTTCTACC ATGCTCGTGT GGCTTGGAGT CATCCGATAC CTCGGTTTCT TTGCAAAGTA 2101 CAACCTCCTC ATTTTGACCC TTCAGGCAGC GCTGCCCAAT GTCATCAGGT TCTGCTGCTG 2161 TGCAGCTATG ATTTACTTAG GTTACTGCTT CTGTGGATGG ATCGTGCTGG GGCCTTACCA 2221 TGACAAGTTT CGTTCTCTGA ACATGGTCTC TGAGTGCCTT TTCTCTCTGA TAAATGGAGA 2281 TGATATGTTT GCCACGTTTG CAAAAATGCA GCAAAAAAGT TACTTAGTCT GGCTGTTTAG 2341 TAGAATTTAC CTCTACTCAT TCATCAGCCT CTTTATATAT ATGATTTTAA GTCTTTTCAT 2401 TGCACTGATC ACTGATACAT ACGAAACAAT TAAGCAATAC CAACAAGATG GCTTCCCAGA 2461 GACTGAACTT CGTACATTTA TATCAGAATG CAAAGATCTA CCCAACTCTG GAAAATACAG 2521 ATTAGAAGAT GACCCTCCAG TATCTTTATT CTGCTGTTGT AAAAAGAAGC TTGGCGGTGG 2581 AGGCAGTGGT GGAGGCGGTA GCATGGGTTC TCATCATCAT CATCATCATG GTATGGCTAG 2641 CATGACTGGT GGACAGCAAA TGGGTCGGGA TCTGTACGAC GATGACGATA AGGATCTCGC 2701 CACCATGGTC GACTCATCAC GTCGTAAGTG GAATAAGACA GGTCACGCAG TCAGAGCTAT 2761 AGGTCGGCTG AGCTCACTCG AGAACGTCTA TATCAAGGCC GACAAGCAGA AGAACGGCAT 2821 CAAGGCGAAC TTCAAGATCC GCCACAACAT CGAGGACGGC GGCGTGCAGC TCGCCTACCA 2881 CTACCAGCAG AACACCCCCA TCGGCGACGG CCCCGTGCTG CTGCCCGACA ACCACTACCT 2941 GAGCGTGCAG TCCAAACTTT CGAAAGACCC CAACGAGAAG CGCGATCACA TGGTCCTGCT 3001 GGAGTTCGTG ACCGCCGCCG GGATCACTCT CGGCATGGAC GAGCTGTACA AGGGCGGTAC 3061 CGGAGGGAGC ATGGTGAGCA AGGGCGAGGA GCTGTTCACC GGGGTGGTGC CCATCCTGGT 3121 CGAGCTGGAC GGCGACGTAA ACGGCCACAA GTTCAGCGTG TCCGGCGAGG GTGAGGGCGA 3181 TGCCACCTAC GGCAAGCTGA CCCTGAAGTT CATCTGCACC ACCGGCAAGC TGCCCGTGCC 3241 CTGGCCCACC CTCGTGACCA CCCTGACCTA CGGCGTGCAG TGCTTCAGCC GCTACCCCGA 3301 CCACATGAAG CAGCACGACT TCTTCAAGTC CGCCATGCCC GAAGGCTACA TCCAGGAGCG 3361 CACCATCTTC TTCAAGGACG ACGGCAACTA CAAGACCCGC GCCGAGGTGA AGTTCGAGGG 3421 CGACACCCTG GTGAACCGCA TCGAGCTGAA GGGCATCGAC TTCAAGGAGG ACGGCAACAT 3481 CCTGGGGCAC AAGCTGGAGT ACAACCTGCC GGACCAACTG ACTGAAGAGC AGATCGCAGA 3541 ATTTAAAGAG GAATTCTCCC TATTTGACAA GGACGGGGAT GGGACAATAA CAACCAAGGA 3601 GCTGGGGACG GTGATGCGGT CTCTGGGGCA GAACCCCACA GAAGCAGAGC TGCAGGACAT 3661 GATCAATGAA GTAGATGCCG ACGGTGACGG CACAATCGAC TTCCCTGAGT TCCTGACAAT 3721 GATGGCAAGA AAAATGAAAT ACAGGGACAC GGAAGAAGAA ATTAGAGAAG CGTTCGGTGT 3781 GTTTGATAAG GATGGCAATG GCTACATCAG TGCAGCAGAG CTTCGCCACG TGATGACAAA 3841 CCTTGGAGAG AAGTTAACAG ATGAAGAGGT TGATGAAATG ATCAGGGAAG CAGACATCGA 3901 TGGGGATGGT CAGGTAAACT ACGAAGAGTT TGTACAAATG ATGACAGCGA AGCTCGAGTA 3961 CCCATACGAC GTACCAGATT ACGCTTAGTC TAGAGGGCCC GTTTAAACCC GCTGATCAGC 4021 CTCGACTGTG CCTTCTAGTT GCCAGCCATC TGTTGTTTGC CCCTCCCCCG TGCCTTCCTT 4081 GACCCTGGAA GGTGCCACTC CCACTGTCCT TTCCTAATAA AATGAGGAAA TTGCATCGCA 4141 TTGTCTGAGT AGGTGTCATT CTATTCTGGG GGGTGGGGTG GGGCAGGACA GCAAGGGGGA 4201 GGATTGGGAA GACAATAGCA GGCATGCTGG GGATGCGGTG GGCTCTATGG CTTCTGAGGC 4261 GGAAAGAACC AGCTGGGGCT CTAGGGGGTA TCCCCACGCG CCCTGTAGCG GCGCATTAAG 4321 CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC 4381 CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC 4441 TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA 4501 AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG 4561 CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC 4621 ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA 4681 TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTAATTCT GTGGAATGTG 4741 TGTCAGTTAG GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG 4801 CATCTCAATT AGTCAGCAAC CAGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT 4861 ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCATAGTCC CGCCCCTAAC TCCGCCCATC 4921 CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT TCTCCGCCCC ATGGCTGACT AATTTTTTTT 4981 ATTTATGCAG AGGCCGAGGC CGCCTCTGCC TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC 5041 TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG CTCCCGGGAG CTTGTATATC CATTTTCGGA 5101 TCTGATCAAG AGACAGGATG AGGATCGTTT CGCATGATTG AACAAGATGG ATTGCACGCA 5161 GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA TTCGGCTATG ACTGGGCACA ACAGACAATC 5221 GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC 5281 AAGACCGACC TGTCCGGTGC CCTGAATGAA CTGCAGGACG AGGCAGCGCG GCTATCGTGG 5341 CTGGCCACGA CGGGCGTTCC TTGCGCAGCT GTGCTCGACG TTGTCACTGA AGCGGGAAGG 5401 GACTGGCTGC TATTGGGCGA AGTGCCGGGG CAGGATCTCC TGTCATCTCA CCTTGCTCCT 5461 GCCGAGAAAG TATCCATCAT GGCTGATGCA ATGCGGCGGC TGCATACGCT TGATCCGGCT 5521 ACCTGCCCAT TCGACCACCA AGCGAAACAT CGCATCGAGC GAGCACGTAC TCGGATGGAA 5581 GCCGGTCTTG TCGATCAGGA TGATCTGGAC GAAGAGCATC AGGGGCTCGC GCCAGCCGAA 5641 CTGTTCGCCA GGCTCAAGGC GCGCATGCCC GACGGCGAGG ATCTCGTCGT GACCCATGGC 5701 GATGCCTGCT TGCCGAATAT CATGGTGGAA AATGGCCGCT TTTCTGGATT CATCGACTGT 5761 GGCCGGCTGG GTGTGGCGGA CCGCTATCAG GACATAGCGT TGGCTACCCG TGATATTGCT 5821 GAAGAGCTTG GCGGCGAATG GGCTGACCGC TTCCTCGTGC TTTACGGTAT CGCCGCTCCC 5881 GATTCGCAGC GCATCGCCTT CTATCGCCTT CTTGACGAGT TCTTCTGAGC GGGACTCTGG 5941 GGTTCGAAAT GACCGACCAA GCGACGCCCA ACCTGCCATC ACGAGATTTC GATTCCACCG 6001 CCGCCTTCTA TGAAAGGTTG GGCTTCGGAA TCGTTTTCCG GGACGCCGGC TGGATGATCC 6061 TCCAGCGCGG GGATCTCATG CTGGAGTTCT TCGCCCACCC CAACTTGTTT ATTGCAGCTT 6121 ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC 6181 TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC TTATCATGTC TGTATACCGT 6241 CGACCTCTAG CTAGAGCTTG GCGTAATCAT GGTCATAGCT GTTTCCTGTG TGAAATTGTT 6301 ATCCGCTCAC AATTCCACAC AACATACGAG CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG 6361 CCTAATGAGT GAGCTAACTC ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG 6421 GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC 6481 GTATTGGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC 6541 GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA 6601 ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG 6661 CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT 6721 CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA 6781 GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC 6841 TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT 6901 AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG 6961 CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG 7021 CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT 7081 TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC 7141 TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG 7201 CTGGTAGCGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG 7261 AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG 7321 GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT 7381 GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT 7441 TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC 7501 TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA 7561 TGATACCGCG AGACCCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG 7621 GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT 7681 GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA 7741 TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT 7801 CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT 7861 TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG 7921 CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG 7981 AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG 8041 CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA 8101 AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT 8161 AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT 8221 GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT 8281 GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA 8341 TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT 8401 TTCCCCGAAA AGTGCCACCT GACGTCGACG GATCGGGAGA TCTCCCGATC CCCTATGGT

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.