pCMV-Rela-3×FLAG-Neo plasmid

pCMV-Rela-3×FLAG-Neo plasmid

PVT86676

pCMV-Rela-3×FLAG-Neo plasmid Information

Plasmid name: pCMV-Rela(mouse)-3×FLAG-Neo
Alias: NM_009045.5;p65; p65 NF-kappa B; p65 NFkB
Gene lenght: 1647bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone: pCMV-T7-MCS-3×FLAG-WPRE-Neo
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-Rela-3×FLAG-Neo plasmid Description

pCMV-Rela-3×FLAG-Neo This plasmid is suitable for overexpression in mammalian cells. The plasmid contains a CMV enhancer and CMV promoter that can jointly initiate the efficient expression of downstream genes, promoting the C-terminal fusion expression of a 3×FLAG tag, which facilitates detection, localization, and purification of the expressed fusion protein using FLAG antibodies. The WPRE protein enhances the processing and stability of the target gene after transcription, helping to increase the half-life and translation efficiency of the mRNA. The plasmid carries a Neo/G418 resistance gene for selecting host cells expressing the target gene.

pCMV-Rela-3×FLAG-Neo plasmid Sequence

1 GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA 61 TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG 121 TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC 181 CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA 241 CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT 301 ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC 361 CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT 421 ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA 481 CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT 541 CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG 601 CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTCTGGC TAACTAGAGA ACCCACTGCT 661 TACTGGCTTA TCGAAATTAA TACGACTCAC TATAGGGAGA CCCAAGCTGG CTAGCGTTTA 721 AACTTAAGCT TGGTACCGAG CTCGAATTCG CCACCATGGA CGATCTGTTT CCCCTCATCT 781 TTCCCTCAGA GCCAGCCCAG GCTTCTGGGC CTTATGTGGA GATCATCGAA CAGCCGAAGC 841 AACGGGGCAT GCGATTCCGC TATAAATGCG AGGGGCGCTC AGCGGGCAGT ATTCCTGGCG 901 AGAGAAGCAC AGATACCACC AAGACACACC CCACCATCAA GATCAATGGC TACACAGGAC 961 CAGGAACAGT TCGAATCTCC CTGGTCACCA AGGATCCACC TCACCGGCCT CATCCACATG 1021 AACTTGTGGG GAAGGACTGC CGGGATGGCT ACTATGAGGC TGACCTCTGC CCAGACCGCA 1081 GTATCCATAG CTTCCAGAAC CTGGGGATCC AGTGTGTGAA GAAGCGAGAC CTGGAGCAAG 1141 CCATTAGCCA GCGAATCCAG ACCAACAATA ACCCCTTTCA CGTTCCTATA GAGGAGCAGC 1201 GCGGGGACTA TGACTTGAAT GCAGTGCGCC TCTGCTTCCA GGTGACAGTG CGGGACCCAG 1261 CAGGCAGGCC CCTCCTCCTG ACCCCTGTCC TCTCACATCC GATTTTTGAT AACCGGGCCC 1321 CCAACACTGC CGAGCTCAAG ATCTGCCGAG TAAACCGGAA CTCTGGGAGC TGCCTCGGTG 1381 GGGATGAGAT CTTCTTGCTG TGCGACAAGG TGCAGAAAGA AGACATTGAG GTGTATTTCA 1441 CGGGACCAGG CTGGGAGGCA CGAGGCTCCT TTTCTCAAGC TGATGTGCAT CGGCAAGTGG 1501 CCATTGTGTT CCGGACTCCT CCGTACGCCG ACCCCAGCCT CCAGGCTCCT GTTCGAGTCT 1561 CCATGCAGCT ACGGCGGCCT TCTGATCGCG AGCTCAGTGA GCCCATGGAG TTCCAGTACT 1621 TGCCAGACAC AGATGATCGC CACCGGATTG AAGAGAAGCG CAAAAGGACC TATGAGACCT 1681 TCAAGAGTAT CATGAAGAAG AGTCCTTTCA ATGGACCAAC TGAACCCCGG CCTCCAACCC 1741 GGCGTATTGC TGTGCCTACC CGAAACTCAA CTTCTGTCCC CAAGCCAGCC CCGCAGCCCT 1801 ACACCTTCCC AGCATCCCTC AGCACCATCA ACTTTGATGA GTTTTCCCCC ATGCTGTTAC 1861 CATCAGGGCA GATCTCAAAC CAGGCCCTGG CCTTGGCACC GTCCTCTGCC CCAGTCCTTG 1921 CCCAGACCAT GGTCCCTTCC TCAGCCATGG TACCTCTGGC TCAGCCCCCA GCTCCTGCCC 1981 CAGTTCTAAC CCCGGGTCCT CCCCAGTCCC TGTCTGCACC TGTTCCAAAG AGCACCCAGG 2041 CTGGGGAAGG CACGCTGTCG GAAGCCCTGC TGCACCTGCA GTTTGATGCT GATGAAGACT 2101 TGGGGGCCTT GCTTGGCAAC AGCACAGACC CAGGAGTGTT CACAGACCTG GCATCTGTGG 2161 ACAACTCAGA GTTTCAGCAG CTCCTGAACC AGGGTGTGTC CATGTCTCAC TCCACAGCTG 2221 AGCCCATGCT GATGGAGTAC CCTGAAGCTA TAACTCGCCT GGTGACAGGG TCCCAGAGGC 2281 CCCCTGACCC AGCTCCCACA CCCCTGGGGA CCTCGGGGCT TCCCAATGGT CTCTCAGGAG 2341 ATGAAGACTT CTCCTCCATT GCGGACATGG ACTTCTCTGC TCTTTTGAGT CAGATCAGCT 2401 CCGGATCCGA CTACAAAGAC CATGACGGTG ATTATAAAGA TCATGACATC GACTACAAGG 2461 ATGACGATGA CAAGTAGTGA GGGCCCGATA TCTCGACAAT CAACCTCTGG ATTACAAAAT 2521 TTGTGAAAGA TTGACTGGTA TTCTTAACTA TGTTGCTCCT TTTACGCTAT GTGGATACGC 2581 TGCTTTAATG CCTTTGTATC ATGCTATTGC TTCCCGTATG GCTTTCATTT TCTCCTCCTT 2641 GTATAAATCC TGGTTGCTGT CTCTTTATGA GGAGTTGTGG CCCGTTGTCA GGCAACGTGG 2701 CGTGGTGTGC ACTGTGTTTG CTGACGCAAC CCCCACTGGT TGGGGCATTG CCACCACCTG 2761 TCAGCTCCTT TCCGGGACTT TCGCTTTCCC CCTCCCTATT GCCACGGCGG AACTCATCGC 2821 CGCCTGCCTT GCCCGCTGCT GGACAGGGGC TCGGCTGTTG GGCACTGACA ATTCCGTGGT 2881 GTTGTCGGGG AAGCTGACGT CCTTTCCATG GCTGCTCGCC TGTGTTGCCA CCTGGATTCT 2941 GCGCGGGACG TCCTTCTGCT ACGTCCCTTC GGCCCTCAAT CCAGCGGACC TTCCTTCCCG 3001 CGGCCTGCTG CCGGCTCTGC GGCCTCTTCC GCGTCTTCGC CTTCGCCCTC AGACGAGTCG 3061 GATCTCCCTT TGGGCCGCCT CCCCGCCTGG AAACGGGGGA GGCTAACTGA AACACGGAAG 3121 GAGACAATAC CGGAAGGAAC CCGCGCTATG ACGGCAATAA AAAGACAGAA TAAAACGCAC 3181 GGGTGTTGGG TCGTTTGTTC ATAAACGCGG GGTTCGGTCC CAGGGCTGGC ACTCTGTCGA 3241 TACCCCACCG AGACCCCATT GGGGCCAATA CGCCCGCGTT TCTTCCTTTT CCCCACCCCA 3301 CCCCCCAAGT TCGGGTGAAG GCCCAGGGCT CGCAGCCAAC GTCGGGGCGG CAGGCCCTGC 3361 CATAGCAGAT CTGCGCAGCT GGGGCTCTAG GGGGTATCCC CACGCGCCCT GTAGCGGCGC 3421 ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT 3481 AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG 3541 TCAAGCTCTA AATCGGGGCA TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA 3601 CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT 3661 TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG 3721 AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGGGGATTTC 3781 GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT AATTCTGTGG 3841 AATGTGTGTC AGTTAGGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA 3901 AGCATGCATC TCAATTAGTC AGCAACCAGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC 3961 AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA TAGTCCCGCC CCTAACTCCG 4021 CCCATCCCGC CCCTAACTCC GCCCAGTTCC GCCCATTCTC CGCCCCATGG CTGACTAATT 4081 TTTTTTATTT ATGCAGAGGC CGAGGCCGCC TCTGCCTCTG AGCTATTCCA GAAGTAGTGA 4141 GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC AAAAAGCTCC CGGGAGCTTG TATATCCATT 4201 TTCGGATCTG ATCAAGAGAC AGGATGAGGA TCGTTTCGCA TGATTGAACA AGATGGATTG 4261 CACGCAGGTT CTCCGGCCGC TTGGGTGGAG AGGCTATTCG GCTATGACTG GGCACAACAG 4321 ACAATCGGCT GCTCTGATGC CGCCGTGTTC CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT 4381 TTTGTCAAGA CCGACCTGTC CGGTGCCCTG AATGAACTGC AGGACGAGGC AGCGCGGCTA 4441 TCGTGGCTGG CCACGACGGG CGTTCCTTGC GCAGCTGTGC TCGACGTTGT CACTGAAGCG 4501 GGAAGGGACT GGCTGCTATT GGGCGAAGTG CCGGGGCAGG ATCTCCTGTC ATCTCACCTT 4561 GCTCCTGCCG AGAAAGTATC CATCATGGCT GATGCAATGC GGCGGCTGCA TACGCTTGAT 4621 CCGGCTACCT GCCCATTCGA CCACCAAGCG AAACATCGCA TCGAGCGAGC ACGTACTCGG 4681 ATGGAAGCCG GTCTTGTCGA TCAGGATGAT CTGGACGAAG AGCATCAGGG GCTCGCGCCA 4741 GCCGAACTGT TCGCCAGGCT CAAGGCGCGC ATGCCCGACG GCGAGGATCT CGTCGTGACC 4801 CATGGCGATG CCTGCTTGCC GAATATCATG GTGGAAAATG GCCGCTTTTC TGGATTCATC 4861 GACTGTGGCC GGCTGGGTGT GGCGGACCGC TATCAGGACA TAGCGTTGGC TACCCGTGAT 4921 ATTGCTGAAG AGCTTGGCGG CGAATGGGCT GACCGCTTCC TCGTGCTTTA CGGTATCGCC 4981 GCTCCCGATT CGCAGCGCAT CGCCTTCTAT CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA 5041 CTCTGGGGTT CGCGAAATGA CCGACCAAGC GACGCCCAAC CTGCCATCAC GAGATTTCGA 5101 TTCCACCGCC GCCTTCTATG AAAGGTTGGG CTTCGGAATC GTTTTCCGGG ACGCCGGCTG 5161 GATGATCCTC CAGCGCGGGG ATCTCATGCT GGAGTTCTTC GCCCACCCCA ACTTGTTTAT 5221 TGCAGCTTAT AATGGTTACA AATAAAGCAA TAGCATCACA AATTTCACAA ATAAAGCATT 5281 TTTTTCACTG CATTCTAGTT GTGGTTTGTC CAAACTCATC AATGTATCTT ATCATGTCTG 5341 TATACCGTCG ACCTCTAGCT AGAGCTTGGC GTAATCATGG TCATAGCTGT TTCCTGTGTG 5401 AAATTGTTAT CCGCTCACAA TTCCACACAA CATACGAGCC GGAAGCATAA AGTGTAAAGC 5461 CTGGGGTGCC TAATGAGTGA GCTAACTCAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT 5521 CCAGTCGGGA AACCTGTCGT GCCAGCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG 5581 CGGTTTGCGT ATTGGGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT 5641 TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 5701 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 5761 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 5821 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 5881 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 5941 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCAATGC TCACGCTGTA GGTATCTCAG 6001 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 6061 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 6121 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 6181 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGGACAGTAT TTGGTATCTG 6241 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 6301 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 6361 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 6421 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 6481 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG 6541 TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT 6601 AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC 6661 CAGTGCTGCA ATGATACCGC GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA 6721 CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA 6781 GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA 6841 CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT 6901 CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC 6961 GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT 7021 CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC 7081 TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG 7141 CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT 7201 CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC 7261 CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG 7321 CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC 7381 ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG 7441 TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT 7501 TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGTCGAC GGATCGGGAG ATCTCCCGAT 7561 CCCCTATGGT CGACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATCTG 7621 CTCCCTGCTT GTGTGTTGGA GGTCGCTGAG TAGTGCGCGA GCAAAATTTA AGCTACAACA 7681 AGGCAAGGCT TGACCGACAA TTGCATGAAG AATCTGCTTA GG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.