pCMV-UBE3C-3×Myc-Neo plasmid

pCMV-UBE3C-3×Myc-Neo plasmid

PVT86670

pCMV-UBE3C-3×Myc-Neo plasmid Information

Plasmid name: pCMV-UBE3C(human)-3×Myc-Neo
Alias: NM_014671.3;HECTH2; RAUL
Gene lenght: 3249bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone: pCMV-MCS-3×Myc-Neo
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-UBE3C-3×Myc-Neo plasmid Description

pCMV-UBE3C-3×Myc-Neo This plasmid is suitable for overexpression in mammalian cells. The plasmid contains a CMV enhancer and a CMV promoter that can jointly initiate efficient expression of downstream genes, promoting the C-terminal fusion expression of a 3×Myc tag, which facilitates recognition and tracking of the Myc-tagged target protein by anti-Myc antibodies in experiments such as Western blotting, immunoprecipitation, or immunofluorescence, thereby studying the expression level, subcellular localization, and interactions with other proteins of the target protein. The plasmid carries a Neo/G418 resistance gene for selecting host cells expressing the target gene.

pCMV-UBE3C-3×Myc-Neo plasmid Sequence

1 GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA 61 TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG 121 TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC 181 CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA 241 CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT 301 ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC 361 CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT 421 ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA 481 CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT 541 CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG 601 CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTGGCTA GCGTTTAAAC TTAAGCTTGG 661 TACCGAGCTC GGATCCACTA GTCCAGTGTG GTGGAATTCG CCACCATGTT CAGCTTCGAA 721 GGCGACTTCA AGACGCGGCC CAAGGTGTCC CTTGGCGGCG CGAGCAGGAA GGAGGAAAAG 781 GCTTCTCTTT TACATCGTAC TCAGGAAGAA AGAAGAAAGA GAGAGGAAGA AAGGCGAAGG 841 TTGAAAAATG CAATAATTAT CCAGTCATTT ATTCGAGGCT ATAGAGACAG AAAACAGCAA 901 TATTCCATCC AAAGAAGTGC ATTTGATCGC TGTGCTACCT TGTCACAGTC CGGGGGCGCT 961 TTTCCCATTG CTAATGGCCC CAACCTTACC CTTTTGGTAA GGCAGCTTCT GTTTTTTTAC 1021 AAACAAAATG AAGACTCAAA ACGTTTGATA TGGCTGTATC AGAACTTAAT TAAACACAGC 1081 TCTCTGTTTG TCAAGCAGTT GGATGGATCT GAGAGACTTA CATGCTTATT TCAGATAAAA 1141 AGATTGATGA GCCTCTGTTG CAGGTTGCTG CAAAACTGTA ATGATGACAG TTTGAATGTT 1201 GCACTTCCAA TGAGAATGCT TGAAGTATTT TCGTCTGAGA ATACTTACTT GCCTGTTTTA 1261 CAAGATGCTA GCTATGTGGT GTCAGTGATT GAACAAATTT TGCACTACAT GATTCACAAT 1321 GGGTATTATA GGTCTCTATA TTTGTTGATT AACAGCAAGC TTCCATCAAG TATTGAATAT 1381 TCTGATTTAT CTCGAGTTCC TATAGCAAAA ATTTTGCTAG AGAATGTTCT AAAACCATTG 1441 CACTTTACTT ACAACTCCTG TCCGGAAGGT GCGAGGCAAC AAGTTTTTAC AGCCTTCACA 1501 GAGGAGTTTC TGGCAGCACC TTTTACAGAT CAGATTTTTC ATTTCATCAT TCCGGCGCTT 1561 GCAGATGCGC AGACCGTTTT CCCTTACGAG CCCTTTCTGA ATGCACTGTT GTTAATAGAG 1621 AGTAGATGTT CAAGAAAGAG TGGTGGAGCA CCCTGGCTTT TCTATTTCGT TTTAACTGTT 1681 GGCGAAAATT ATTTGGGGGC CCTCTCTGAG GAAGGGCTGC TGGTGTATTT GCGGGTGCTG 1741 CAGACCTTCC TCTCTCAGTT ACCAGTCTCT CCTGCCAGCG CGAGCTGTCA CGACTCAGCC 1801 AGTGACTCTG AGGAGGAGAG TGAAGAAGCC GACAAGCCCT CAAGCCCGGA GGATGGCAGA 1861 CTGTCAGTAT CATACATAAC AGAGGAATGC CTGAAGAAGC TGGACACAAA GCAGCAGACC 1921 AACACCCTGC TCAACCTGGT GTGGAGGGAC TCTGCGAGCG AGGAGGTCTT CACCACCATG 1981 GCCTCCGTCT GCCACACGCT GATGGTGCAG CACCGCATGA TGGTACCCAA AGTCAGGCTT 2041 CTCTACAGTT TAGCCTTTAA TGCCAGGTTT CTGAGACATC TTTGGTTTCT AATATCTTCC 2101 ATGTCAACAC GGATGATCAC AGGGTCTATG GTACCGTTGC TTCAGGTGAT ATCCAGGGGT 2161 TCTCCTATGT CTTTTGAAGA TTCTAGTCGA ATCATCCCAC TCTTTTATCT TTTTAGCTCC 2221 TTGTTTAGTC ATTCACTAAT TTCCATACAT GATAACGAAT TCTTCGGTGA TCCCATAGAA 2281 GTTGTAGGTC AAAGACAATC ATCAATGATG CCTTTTACTT TAGAAGAGCT GATAATGTTG 2341 TCTCGATGCC TTCGAGATGC ATGCCTGGGG ATCATCAAGT TGGCTTATCC AGAAACCAAG 2401 CCAGAAGTTC GAGAAGAATA TATTACAGCA TTTCAGAGTA TTGGAGTTAC TACTAGCTCT 2461 GAAATGCAAC AATGCATACA GATGGAACAG AAAAGATGGA TTCAGTTATT TAAGGTTATC 2521 ACCAATCTAG TGAAAATGTT GAAGTCCAGA GACACGAGGA GAAATTTTTG TCCTCCAAAC 2581 CACTGGCTGT CAGAACAAGA AGATATTAAA GCAGATAAGG TCACTCAGCT CTATGTGCCA 2641 GCATCCAGAC ATGTGTGGAG GTTCCGGCGG ATGGGGAGGA TAGGCCCGCT GCAGTCCACC 2701 CTGGACGTGG GTTTGGAGTC CCCGCCGCTG TCTGTGTCTG AGGAAAGACA GCTTGCTGTC 2761 CTGACAGAGT TGCCTTTTGT GGTTCCATTT GAGGAACGAG TAAAGATCTT TCAGAGGTTG 2821 ATTTATGCAG ATAAGCAAGA AGTTCAAGGA GATGGTCCAT TTCTGGATGG AATTAATGTC 2881 ACAATAAGAA GAAATTACAT TTATGAAGAT GCTTATGACA AACTTTCTCC AGAAAATGAG 2941 CCTGATTTGA AAAAGCGGAT CCGTGTGCAC TTGCTCAATG CCCATGGCCT GGATGAAGCT 3001 GGCATTGATG GTGGTGGTAT TTTCAGAGAG TTTTTAAATG AACTACTGAA GTCAGGATTT 3061 AACCCCAACC AGGGGTTCTT TAAGACTACT AATGAAGGGC TTCTGTACCC CAACCCGGCT 3121 GCTCAGATGC TTGTGGGAGA TTCTTTTGCC AGACATTACT ACTTCCTAGG CAGAATGCTT 3181 GGAAAGGCTC TCTATGAGAA CATGCTGGTG GAGCTGCCCT TTGCAGGCTT CTTTCTTTCC 3241 AAGTTGCTTG GAACCAGTGC CGACGTGGAC ATTCACCACC TCGCCTCCCT AGACCCTGAG 3301 GTGTATAAGA ATTTGCTCTT TCTGAAGAGC TACGAAGACG ATGTGGAGGA GCTTGGGCTG 3361 AACTTCACTG TGGTGAACAA TGACCTGGGA GAGGCGCAGG TAGTTGAACT AAAATTCGGT 3421 GGGAAAGACA TCCCTGTCAC CAGCGCCAAC CGGATTGCGT ACATCCACTT GGTGGCAGAC 3481 TACAGGCTGA ACAGGCAGAT CCGCCAGCAC TGCCTGGCTT TCCGCCAGGG CCTTGCCAAT 3541 GTCGTCAGCC TCGAGTGGCT CCGAATGTTT GATCAGCAAG AAATTCAGGT ATTAATTTCT 3601 GGTGCACAAG TTCCCATAAG CCTAGAGGAC CTAAAATCCT TTACAAACTA TTCAGGAGGC 3661 TATTCTGCAG ACCATCCTGT TATTAAGGTC TTCTGGAGAG TTGTGGAAGG GTTCACTGAT 3721 GAAGAAAAGC GCAAACTGCT GAAGTTTGTA ACAAGCTGCT CTCGACCCCC TCTCTTGGGG 3781 TTTAAGGAGT TGTATCCCGC ATTTTGTATT CACAACGGAG GCTCCGACCT TGAGCGGCTC 3841 CCCACAGCCA GCACCTGCAT GAACCTGCTG AAGCTCCCCG AGTTCTATGA CGAGACACTT 3901 TTGCGAAGTA AACTTCTCTA TGCGATTGAA TGTGCCGCTG GCTTTGAGCT GAGCCTCGAG 3961 GAACAAAAAC TAATATCAGA GGAAGATTTG GAACAGAAAC TAATCAGTGA AGAAGATTTA 4021 GAGCAAAAGT TAATTTCCGA GGAGGACTTA TAGTGAGGGC CCGATATCTC GACAATCAAC 4081 CTCTGGATTA CAAAATTTGT GAAAGATTGA CTGGTATTCT TAACTATGTT GCTCCTTTTA 4141 CGCTATGTGG ATACGCTGCT TTAATGCCTT TGTATCATGC TATTGCTTCC CGTATGGCTT 4201 TCATTTTCTC CTCCTTGTAT AAATCCTGGT TGCTGTCTCT TTATGAGGAG TTGTGGCCCG 4261 TTGTCAGGCA ACGTGGCGTG GTGTGCACTG TGTTTGCTGA CGCAACCCCC ACTGGTTGGG 4321 GCATTGCCAC CACCTGTCAG CTCCTTTCCG GGACTTTCGC TTTCCCCCTC CCTATTGCCA 4381 CGGCGGAACT CATCGCCGCC TGCCTTGCCC GCTGCTGGAC AGGGGCTCGG CTGTTGGGCA 4441 CTGACAATTC CGTGGTGTTG TCGGGGAAGC TGACGTCCTT TCCATGGCTG CTCGCCTGTG 4501 TTGCCACCTG GATTCTGCGC GGGACGTCCT TCTGCTACGT CCCTTCGGCC CTCAATCCAG 4561 CGGACCTTCC TTCCCGCGGC CTGCTGCCGG CTCTGCGGCC TCTTCCGCGT CTTCGCCTTC 4621 GCCCTCAGAC GAGTCGGATC TCCCTTTGGG CCGCCTCCCC GCCTGGAAAC GGGGGAGGCT 4681 AACTGAAACA CGGAAGGAGA CAATACCGGA AGGAACCCGC GCTATGACGG CAATAAAAAG 4741 ACAGAATAAA ACGCACGGGT GTTGGGTCGT TTGTTCATAA ACGCGGGGTT CGGTCCCAGG 4801 GCTGGCACTC TGTCGATACC CCACCGAGAC CCCATTGGGG CCAATACGCC CGCGTTTCTT 4861 CCTTTTCCCC ACCCCACCCC CCAAGTTCGG GTGAAGGCCC AGGGCTCGCA GCCAACGTCG 4921 GGGCGGCAGG CCCTGCCATA GCAGATCTGC GCAGCTGGGG CTCTAGGGGG TATCCCCACG 4981 CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA 5041 CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT 5101 TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGCATCCC TTTAGGGTTC CGATTTAGTG 5161 CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT 5221 CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC 5281 TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG 5341 GGATTTTGGG GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG 5401 CGAATTAATT CTGTGGAATG TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG GCTCCCCAGC 5461 AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCAGGTGTG GAAAGTCCCC 5521 AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCATAGT 5581 CCCGCCCCTA ACTCCGCCCA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC 5641 CCATGGCTGA CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCTG CCTCTGAGCT 5701 ATTCCAGAAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA AGCTCCCGGG 5761 AGCTTGTATA TCCATTTTCG GATCTGATCA AGAGACAGGA TGAGGATCGT TTCGCATGAT 5821 TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC TATTCGGCTA 5881 TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC TGTCAGCGCA 5941 GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG AACTGCAGGA 6001 CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG CTGTGCTCGA 6061 CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG GGCAGGATCT 6121 CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG CAATGCGGCG 6181 GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC ATCGCATCGA 6241 GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG ACGAAGAGCA 6301 TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGCGCATGC CCGACGGCGA 6361 GGATCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG AAAATGGCCG 6421 CTTTTCTGGA TTCATCGACT GTGGCCGGCT GGGTGTGGCG GACCGCTATC AGGACATAGC 6481 GTTGGCTACC CGTGATATTG CTGAAGAGCT TGGCGGCGAA TGGGCTGACC GCTTCCTCGT 6541 GCTTTACGGT ATCGCCGCTC CCGATTCGCA GCGCATCGCC TTCTATCGCC TTCTTGACGA 6601 GTTCTTCTGA GCGGGACTCT GGGGTTCGCG AAATGACCGA CCAAGCGACG CCCAACCTGC 6661 CATCACGAGA TTTCGATTCC ACCGCCGCCT TCTATGAAAG GTTGGGCTTC GGAATCGTTT 6721 TCCGGGACGC CGGCTGGATG ATCCTCCAGC GCGGGGATCT CATGCTGGAG TTCTTCGCCC 6781 ACCCCAACTT GTTTATTGCA GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT 6841 TCACAAATAA AGCATTTTTT TCACTGCATT CTAGTTGTGG TTTGTCCAAA CTCATCAATG 6901 TATCTTATCA TGTCTGTATA CCGTCGACCT CTAGCTAGAG CTTGGCGTAA TCATGGTCAT 6961 AGCTGTTTCC TGTGTGAAAT TGTTATCCGC TCACAATTCC ACACAACATA CGAGCCGGAA 7021 GCATAAAGTG TAAAGCCTGG GGTGCCTAAT GAGTGAGCTA ACTCACATTA ATTGCGTTGC 7081 GCTCACTGCC CGCTTTCCAG TCGGGAAACC TGTCGTGCCA GCTGCATTAA TGAATCGGCC 7141 AACGCGCGGG GAGAGGCGGT TTGCGTATTG GGCGCTCTTC CGCTTCCTCG CTCACTGACT 7201 CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC 7261 GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA 7321 AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG 7381 ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA 7441 GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC 7501 TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CAATGCTCAC 7561 GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC 7621 CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG 7681 TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT 7741 ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGGA 7801 CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT 7861 CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA 7921 TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG 7981 CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT 8041 TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT 8101 AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC 8161 TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG 8221 GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG 8281 ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT 8341 TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG 8401 TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT 8461 TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA 8521 TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG 8581 CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT 8641 CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA 8701 TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA 8761 GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT 8821 TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT 8881 CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA 8941 AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT 9001 GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA 9061 ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC GTCGACGGAT 9121 CGGGAGATCT CCCGATCCCC TATGGTCGAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA 9181 GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT GCGCGAGCAA 9241 AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC TGCTTAGG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.