pLV3-CMV-SRCAP-3×FLAG-CopGFP-P

pLV3-CMV-SRCAP-3×FLAG-CopGFP-Puro plasmid

PVT86250

pLV3-CMV-SRCAP-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-SRCAP(human)-3×FLAG-CopGFP-Puro
Alias: NM_006662.3;DEHMBA; DOMO1; EAF1; FLHS; SWR1
Gene lenght: 9690bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone: pLV3-CMV-MCS-3×FLAG-CopGFP-Puro
Forward primer: CMV-F
Reverse primer:
Copy number: Low
Induction:

pLV3-CMV-SRCAP-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-SRCAP-3×FLAG-CopGFP-Puro This plasmid is suitable for overexpression in mammalian cells. The CMV strong promoter carried by the plasmid can initiate the transcription of the human SRCAP gene, promoting the C-terminal fusion expression of the SRCAP gene with a 3×FLAG tag, facilitating the detection, localization, and purification of the expressed fusion protein using FLAG antibodies. Additionally, the plasmid contains HIV-1 Ψ and 3'/5' LTR elements for lentivirus packaging, facilitating the stable integration of the gene into host cells. The CopGFP green fluorescent protein carried by the plasmid is used to observe the expression and localization of the target gene. The plasmid carries a Puro resistance gene for the selection of host cells expressing the target gene.

pLV3-CMV-SRCAP-3×FLAG-CopGFP-Puro plasmid Sequence

1 ACGCGTGTAG TCTTATGCAA TACTCTTGTA GTCTTGCAAC ATGGTAACGA TGAGTTAGCA 61 ACATGCCTTA CAAGGAGAGA AAAAGCACCG TGCATGCCGA TTGGTGGAAG TAAGGTGGTA 121 CGATCGTGCC TTATTAGGAA GGCAACAGAC GGGTCTGACA TGGATTGGAC GAACCACTGA 181 ATTGCCGCAT TGCAGAGATA TTGTATTTAA GTGCCTAGCT CGATACAATA AACGGGTCTC 241 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 301 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 361 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 421 CCCGAACAGG GACCTGAAAG CGAAAGGGAA ACCAGAGCTC TCTCGACGCA GGACTCGGCT 481 TGCTGAAGCG CGCACGGCAA GAGGCGAGGG GCGGCGACTG GTGAGTACGC CAAAAATTTT 541 GACTAGCGGA GGCTAGAAGG AGAGAGATGG GTGCGAGAGC GTCAGTATTA AGCGGGGGAG 601 AATTAGATCG CGATGGGAAA AAATTCGGTT AAGGCCAGGG GGAAAGAAAA AATATAAATT 661 AAAACATATA GTATGGGCAA GCAGGGAGCT AGAACGATTC GCAGTTAATC CTGGCCTGTT 721 AGAAACATCA GAAGGCTGTA GACAAATACT GGGACAGCTA CAACCATCCC TTCAGACAGG 781 ATCAGAAGAA CTTAGATCAT TATATAATAC AGTAGCAACC CTCTATTGTG TGCATCAAAG 841 GATAGAGATA AAAGACACCA AGGAAGCTTT AGACAAGATA GAGGAAGAGC AAAACAAAAG 901 TAAGACCACC GCACAGCAAG CGGCCACTGA TCTTCAGACC TGGAGGAGGA GATATGAGGG 961 ACAATTGGAG AAGTGAATTA TATAAATATA AAGTAGTAAA AATTGAACCA TTAGGAGTAG 1021 CACCCACCAA GGCAAAGAGA AGAGTGGTGC AGAGAGAAAA AAGAGCAGTG GGAATAGGAG 1081 CTTTGTTCCT TGGGTTCTTG GGAGCAGCAG GAAGCACTAT GGGCGCAGCC TCAATGACGC 1141 TGACGGTACA GGCCAGACAA TTATTGTCTG GTATAGTGCA GCAGCAGAAC AATTTGCTGA 1201 GGGCTATTGA GGCGCAACAG CATCTGTTGC AACTCACAGT CTGGGGCATC AAGCAGCTCC 1261 AGGCAAGAAT CCTGGCTGTG GAAAGATACC TAAAGGATCA ACAGCTCCTG GGGATTTGGG 1321 GTTGCTCTGG AAAACTCATT TGCACCACTG CTGTGCCTTG GAATGCTAGT TGGAGTAATA 1381 AATCTCTGGA ACAGATTGGA ATCACACGAC CTGGATGGAG TGGGACAGAG AAATTAACAA 1441 TTACACAAGC TTAATACACT CCTTAATTGA AGAATCGCAA AACCAGCAAG AAAAGAATGA 1501 ACAAGAATTA TTGGAATTAG ATAAATGGGC AAGTTTGTGG AATTGGTTTA ACATAACAAA 1561 TTGGCTGTGG TATATAAAAT TATTCATAAT GATAGTAGGA GGCTTGGTAG GTTTAAGAAT 1621 AGTTTTTGCT GTACTTTCTA TAGTGAATAG AGTTAGGCAG GGATATTCAC CATTATCGTT 1681 TCAGACCCAC CTCCCAACCC CGAGGGGACC CGACAGGCCC GAAGGAATAG AAGAAGAAGG 1741 TGGAGAGAGA GACAGAGACA GATCCATTCG ATTAGTGAAC GGATCTCGAC GGTATCGGTT 1801 AACTTTTAAA AGAAAAGGGG GGATTGGGGG GTACAGTGCA GGGGAAAGAA TAGTAGACAT 1861 AATAGCAACA GACATACAAA CTAAAGAATT ACAAAAACAA ATTACAAAAA TTCAAAATTT 1921 TATCGATACT AGTATTATGC CCAGTACATG ACCTTATGGG ACTTTCCTAC TTGGCAGTAC 1981 ATCTACGTAT TAGTCATCGC TATTACCATG GTGATGCGGT TTTGGCAGTA CATCAATGGG 2041 CGTGGATAGC GGTTTGACTC ACGGGGATTT CCAAGTCTCC ACCCCATTGA CGTCAATGGG 2101 AGTTTGTTTT GGCACCAAAA TCAACGGGAC TTTCCAAAAT GTCGTAACAA CTCCGCCCCA 2161 TTGACGCAAA TGGGCGGTAG GCGTGTACGG TGGGAGGTTT ATATAAGCAG AGCTCGTTTA 2221 GTGAACCGTC AGATCGCCTG GAGACGCCAT CCACGCTGTT TTGACCTCCA TAGAAGATTC 2281 TAGAGCTAGC CTGATACGAA CTCGGAATTC GCCACCATGC AGAGCAGCCC CTCCCCTGCT 2341 CACCCTCAGC TCCCAGTCCT ACAGACACAG ATGGTGTCGG ACGGCATGAC AGGCAGCAAT 2401 CCTGTGTCCC CTGCCTCATC CAGTTCCCCA GCCTCTAGTG GGGCAGGCGG CATCTCCCCG 2461 CAGCACATAG CTCAAGATTC CTCACTGGAT GGACCTCCAG GCCCCCCAGA TGGTGCCACA 2521 GTGCCCCTGG AGGGGTTCAG CTTATCCCAG GCTGCTGACC TGGCTAACAA GGGCCCGAAG 2581 TGGGAGAAGA GCCATGCCGA AATTGCAGAA CAGGCCAAGC ATGAGGCCGA GATCGAGACT 2641 CGGATTGCTG AGCTGCGGAA GGAGGGTTTC TGGTCACTGA AGAGGCTGCC TAAGGTGCCA 2701 GAGCCCCCTC GCCCCAAAGG TCACTGGGAC TATTTGTGCG AAGAGATGCA GTGGCTCTCT 2761 GCTGACTTTG CTCAGGAGCG CCGTTGGAAA CGGGGTGTGG CCCGGAAGGT GGTGCGCATG 2821 GTGATCCGGC ACCACGAGGA GCAGCGGCAG AAAGAGGAAC GGGCCCGGAG GGAGGAGCAG 2881 GCCAAGCTGC GTCGAATTGC TTCCACCATG GCCAAGGATG TCAGGCAGTT CTGGAGCAAT 2941 GTGGAGAAGG TGGTGCAATT CAAGCAACAG TCCCGGCTTG AGGAAAAGCG CAAAAAAGCC 3001 CTGGACCTGC ATTTGGACTT CATTGTGGGG CAAACTGAAA AGTACTCGGA CCTTCTGTCT 3061 CAGAGCCTCA ACCAGCCATT AACCTCCAGC AAAGCAGGCT CTTCCCCTTG CCTCGGCTCT 3121 TCCTCAGCTG CCTCCAGTCC TCCACCCCCT GCTTCTCGCC TGGATGATGA AGATGGGGAC 3181 TTTCAACCCC AAGAGGATGA GGAAGAGGAT GATGAGGAAA CGATTGAAGT TGAAGAACAA 3241 CAGGAAGGCA ATGATGCAGA GGCCCAGAGG CGTGAGATTG AGCTGCTTCG CCGTGAGGGA 3301 GAATTGCCAC TGGAAGAGCT GCTCCGTTCC CTTCCCCCTC AGCTGTTGGA AGGGCCTTCC 3361 AGCCCCTCTC AAACCCCCTC ATCTCATGAT AGTGACACCC GAGATGGGCC TGAAGAAGGT 3421 GCTGAAGAAG AGCCCCCTCA GGTGTTGGAG ATAAAGCCCC CACCCTCTGC TGTCACACAG 3481 CGCAACAAAC AGCCTTGGCA TCCAGATGAA GATGATGAAG AGTTTACTGC CAACGAAGAG 3541 GAAGCGGAGG ATGAAGAGGA TACTATAGCA GCTGAGGAAC AGTTGGAAGG GGAGGTGGAT 3601 CATGCCATGG AGCTGAGCGA GTTGGCTCGA GAAGGTGAGC TTTCCATGGA GGAGCTATTG 3661 CAGCAGTATG CAGGAGCCTA TGCCCCAGGC TCTGGGAGCA GTGAAGATGA GGATGAAGAT 3721 GAGGTTGATG CTAATAGCTC TGACTGTGAA CCAGAGGGGC CCGTGGAAGC GGAAGAGCCT 3781 CCTCAGGAGG ATAGTAGCAG TCAGTCAGAC TCTGTGGAGG ACCGGAGTGA GGATGAGGAA 3841 GATGAACATT CAGAGGAGGA AGAAACAAGT GGAAGTTCAG CATCAGAGGA ATCTGAGTCT 3901 GAAGAGTCTG AGGATGCCCA ATCACAGAGC CAAGCAGATG AAGAGGAGGA AGATGATGAT 3961 TTTGGGGTGG AGTACTTGCT TGCCAGGGAT GAAGAGCAGA GTGAGGCAGA TGCAGGCAGT 4021 GGGCCTCCTA CTCCAGGGCC CACTACTCTA GGTCCAAAGA AAGAAATTAC TGACATTGCT 4081 GCAGCAGCTG AAAGTCTCCA GCCCAAGGGT TACACGCTGG CCACGACCCA GGTAAAGACG 4141 CCCATTCCCC TGCTTCTGCG GGGCCAGCTC CGGGAGTACC AGCACATTGG GCTAGACTGG 4201 CTGGTTACCA TGTATGAGAA GAAGCTTAAT GGCATTCTTG CTGATGAGAT GGGGCTTGGG 4261 AAGACCATCC AGACCATCTC TCTGCTTGCC CACTTGGCTT GTGAGAAAGG TAACTGGGGT 4321 CCCCATTTAA TCATTGTTCC CACCAGCGTG ATGTTGAACT GGGAGATGGA GTTGAAACGG 4381 TGGTGCCCCA GCTTTAAAAT CCTCACTTAC TATGGAGCCC AGAAAGAGAG GAAGCTCAAG 4441 CGGCAGGGCT GGACCAAGCC CAATGCCTTT CATGTGTGTA TCACATCTTA CAAGCTGGTG 4501 CTGCAGGACC ACCAGGCCTT CCGTCGCAAG AACTGGCGCT ATCTCATTCT GGATGAGGCG 4561 CAGAACATCA AGAACTTCAA GTCACAGCGC TGGCAGTCAC TCCTCAACTT CAACAGCCAG 4621 AGACGCCTGC TCCTGACAGG AACTCCCTTG CAGAACAGCC TCATGGAGCT GTGGTCCTTG 4681 ATGCACTTTT TGATGCCCCA TGTCTTCCAG TCTCATCGCG AGTTCAAGGA GTGGTTCTCT 4741 AATCCCCTAA CTGGCATGAT TGAGGGCAGC CAAGAGTATA ATGAAGGTCT AGTCAAACGC 4801 CTCCACAAGG TTTTGAGGCC TTTTTTACTG CGCCGAGTTA AGGTGGATGT TGAGAAGCAG 4861 ATGCCCAAAA AGTACGAGCA TGTTATCCGC TGCAGGCTCT CCAAGCGTCA ACGCTGTCTC 4921 TATGATGACT TCATGGCACA GACCACAACT AAGGAGACAC TAGCCACAGG CCATTTCATG 4981 AGCGTCATCA ACATTTTGAT GCAGCTGAGA AAAGTTTGCA ATCATCCAAA TCTGTTCGAC 5041 CCTCGACCGG TTACCTCCCC TTTCATCACC CCAGGCATCT GCTTCAGCAC CGCCTCTCTG 5101 GTGCTAAGGG CCACGGATGT CCATCCCCTC CAGCGGATAG ACATGGGTCG ATTTGACCTT 5161 ATTGGCCTGG AAGGTCGTGT CTCTCGATAT GAGGCAGACA CATTTCTGCC CCGGCACCGC 5221 CTCTCTCGCC GGGTACTGTT AGAAGTGGCT ACTGCTCCTG ACCCCCCACC CCGGCCCAAG 5281 CCAGTCAAGA TGAAGGTCAA CAGGATGCTG CAGCCAGTAC CTAAGCAAGA AGGCCGGACA 5341 GTGGTGGTGG TGAACAACCC ACGGGCGCCC CTGGGCCCTG TCCCAGTTCG ACCTCCTCCA 5401 GGTCCTGAGC TCTCAGCCCA GCCCACCCCT GGCCCAGTCC CCCAAGTGCT GCCAGCATCA 5461 CTGATGGTTT CAGCCTCACC TGCCGGGCCC CCGCTTATTC CTGCATCTCG GCCTCCTGGC 5521 CCTGTCCTCT TGCCTCCACT GCAGCCCAAC AGTGGTTCTC TCCCCCAGGT GTTGCCATCC 5581 CCCCTGGGGG TCCTGAGTGG GACCTCACGG CCTCCCACGC CAACCTTGTC CCTAAAGCCA 5641 ACACCACCTG CCCCAGTTCG CCTGAGCCCA GCCCCACCTC CAGGCTCCTC TAGCCTGTTG 5701 AAGCCCCTGA CAGTGCCACC AGGCTACACC TTCCCTCCTG CTGCTGCCAC CACCACTTCT 5761 ACCACCACGG CAACTGCTAC CACCACAGCA GTGCCAGCTC CGACTCCTGC ACCACAGCGC 5821 CTCATTCTAT CTCCCGATAT GCAGGCTCGC CTGCCCTCAG GCGAAGTGGT CAGCATCGGG 5881 CAGTTAGCCT CACTGGCACA ACGTCCAGTG GCTAATGCAG GGGGAAGCAA ACCTCTCACC 5941 TTCCAAATCC AGGGCAACAA GCTGACTTTG ACTGGTGCCC AGGTGCGCCA GCTTGCTGTG 6001 GGGCAGCCCC GCCCGCTGCA AAGGAATGTG GTGCACCTCG TGTCAGCAGG GGGGCAGCAC 6061 CATCTCATCA GCCAGCCTGC CCATGTGGCC CTCATCCAGG CCGTGGCCCC GACCCCTGGC 6121 CCTACCCCTG TCTCTGTGCT GCCTTCTTCG ACCCCCAGCA CCACCCCTGC CCCTACTGGC 6181 CTCAGCCTTC CGCTTGCTGC TAACCAGGTG CCACCAACCA TGGTGAATAA TACAGGCGTG 6241 GTGAAGATTG TAGTGAGACA AGCCCCTCGG GATGGACTGA CTCCTGTTCC TCCATTGGCC 6301 CCAGCACCCC GGCCTCCGAG CTCTGGGCTT CCAGCTGTGT TGAATCCACG CCCCACGTTA 6361 ACCCCTGGCC GGCTACCCAC ACCTACTCTG GGTACTGCTC GAGCCCCCAT GCCCACACCC 6421 ACTCTGGTGA GGCCTCTTCT CAAGCTGGTC CACAGTCCTT CACCTGAAGT CAGTGCTTCA 6481 GCCCCCGGAG CTGCCCCCTT GACCATCTCT TCTCCTCTCC ACGTGCCATC CTCCCTCCCT 6541 GGGCCAGCCT CTTCTCCAAT GCCAATTCCC AACTCCTCTC CCCTTGCTAG TCCTGTGTCC 6601 TCTACAGTCT CAGTTCCATT GTCATCTTCA CTCCCCATCT CTGTCCCCAC CACACTTCCT 6661 GCCCCAGCCT CGGCTCCACT CACCATCCCC ATCTCAGCCC CCTTGACTGT TTCTGCTTCG 6721 GGCCCAGCTC TGTTGACCAG TGTGACTCCA CCATTGGCAC CTGTTGTCCC AGCGGCTCCT 6781 GGACCTCCCT CCTTGGCACC ATCTGGTGCT TCCCCGTCAG CATCAGCCTT GACTCTAGGT 6841 TTGGCCACAG CTCCATCCCT GTCTTCATCT CAGACACCTG GTCACCCTCT GTTGTTGGCT 6901 CCCACCTCTT CACATGTTCC AGGGTTGAAC TCAACCGTGG CCCCAGCATG CTCACCTGTC 6961 CTGGTGCCAG CTTCGGCTCT GGCCAGTCCT TTTCCGTCAG CACCAAATCC AGCTCCAGCT 7021 CAGGCTTCCC TTCTGGCTCC AGCATCTTCT GCATCTCAGG CTCTAGCCAC CCCTCTGGCT 7081 CCTATGGCGG CTCCACAGAC AGCAATTCTG GCTCCTTCTC CAGCTCCTCC TCTGGCTCCT 7141 CTTCCGGTCC TGGCACCATC GCCAGGTGCT GCTCCTGTCC TGGCTTCATC ACAGACTCCG 7201 GTTCCAGTTA TGGCTCCATC GTCTACTCCA GGAACCTCTT TAGCCTCAGC TTCACCGGTA 7261 CCAGCTCCAA CCCCTGTGTT GGCTCCATCA TCAACTCAAA CTATGCTACC AGCCCCGGTT 7321 CCGTCACCTC TCCCGAGCCC GGCTTCTACG CAGACACTGG CCCTAGCCCC AGCTTTAGCA 7381 CCCACTCTTG GAGGCTCATC TCCATCTCAG ACACTCTCTT TGGGAACGGG GAACCCCCAG 7441 GGACCCTTTC CAACTCAGAC ATTGTCATTA ACTCCAGCAT CATCCCTGGT ACCAACTCCA 7501 GCCCAGACAC TGTCTTTGGC ACCAGGACCA CCACTGGGTC CAACTCAGAC GCTGTCTCTG 7561 GCTCCAGCAC CCCCTCTGGC TCCAGCTTCT CCAGTGGGCC CAGCCCCAGC TCACACGCTG 7621 ACTTTGGCTC CAGCATCGTC ATCTGCTTCA CTCCTGGCCC CAGCTTCAGT GCAGACACTG 7681 ACCTTGAGCC CTGCCCCAGT TCCTACCCTG GGCCCGGCCG CAGCTCAGAC CTTGGCGCTG 7741 GCCCCAGCCT CCACACAGTC CCCAGCTTCC CAGGCATCTT CCCTTGTGGT TTCGGCATCT 7801 GGTGCCGCTC CCTTGCCTGT CACCATGGTA TCCCGGCTGC CTGTTTCCAA GGATGAGCCT 7861 GACACACTGA CATTGCGCTC TGGTCCCCCC AGCCCTCCCT CCACTGCTAC CTCGTTTGGT 7921 GGCCCCCGGC CTCGACGCCA GCCCCCCCCA CCACCTCGTT CCCCTTTTTA TCTGGACTCC 7981 CTGGAGGAAA AGCGGAAGCG GCAGCGGTCT GAACGCCTGG AACGGATTTT CCAACTTAGT 8041 GAGGCTCATG GGGCCCTGGC ACCTGTGTAT GGGACTGAAG TCCTGGATTT CTGTACCCTG 8101 CCCCAACCTG TTGCCAGCCC CATCGGCCCT CGTTCTCCTG GCCCCAGCCA CCCCACCTTT 8161 TGGACTTATA CCGAGGCTGC CCACCGGGCT GTACTGTTTC CCCAGCAGCG ACTAGACCAG 8221 CTGTCAGAAA TCATTGAGAG GTTCATCTTT GTCATGCCTC CTGTGGAGGC ACCTCCCCCT 8281 TCCCTGCATG CCTGCCACCC ACCTCCTTGG CTGGCCCCAC GTCAGGCAGC CTTCCAGGAG 8341 CAATTGGCCT CTGAGCTCTG GCCCCGGGCT CGTCCTTTGC ACCGTATTGT GTGTAACATG 8401 CGCACCCAGT TCCCTGACTT AAGACTCATC CAGTATGATT GCGGAAAGTT GCAGACGTTG 8461 GCAGTGCTGT TGCGGCAGCT CAAGGCAGAG GGCCACCGAG TGCTCATCTT CACCCAGATG 8521 ACCCGAATGC TGGATGTATT GGAGCAGTTT CTCACCTACC ATGGCCATCT CTACCTGCGC 8581 CTGGATGGAT CTACTAGAGT TGAACAGAGA CAGGCCTTGA TGGAACGGTT CAATGCAGAC 8641 AAACGCATAT TCTGCTTCAT CCTTTCAACT CGGAGTGGGG GTGTGGGCGT GAACCTGACA 8701 GGAGCAGACA CTGTTGTTTT TTATGACAGC GACTGGAATC CCACCATGGA TGCTCAGGCC 8761 CAGGACCGCT GTCACCGAAT TGGCCAGACC CGGGATGTCC ACATATATAG GCTTATCAGT 8821 GAACGGACAG TGGAGGAGAA CATCCTAAAA AAGGCAAATC AGAAGAGAAT GTTGGGGGAC 8881 ATGGCCATTG AGGGAGGCAA CTTCACCACA GCCTATTTCA AACAGCAGAC CATCCGAGAG 8941 CTGTTTGATA TGCCCCTGGA GGAACCTTCT AGCTCATCCG TGCCCTCTGC CCCTGAAGAG 9001 GAGGAAGAGA CTGTGGCCAG CAAGCAGACT CATATTCTGG AGCAGGCATT GTGTCGGGCA 9061 GAAGATGAAG AGGATATCCG TGCAGCCACC CAGGCCAAGG CTGAACAGGT GGCTGAGCTT 9121 GCAGAATTTA ATGAGAACGA TGGGTTTCCT GCTGGTGAGG GAGAGGAAGC TGGCCGGCCT 9181 GGGGCTGAGG ATGAGGAGAT GTCCCGGGCT GAGCAGGAAA TTGCTGCCCT CGTAGAACAG 9241 CTGACCCCCA TTGAGCGCTA TGCCATGAAA TTCCTGGAGG CCTCACTGGA GGAGGTGAGC 9301 CGAGAGGAGC TCAAACAGGC AGAAGAGCAA GTGGAAGCTG CCCGCAAAGA CCTGGACCAA 9361 GCCAAGGAGG AGGTGTTCCG CCTACCCCAA GAGGAGGAGG AGGGGCCGGG GGCTGGGGAT 9421 GAGAGTTCCT GTGGGACTGG TGGAGGCACC CACCGGCGCA GTAAAAAGGC CAAAGCCCCT 9481 GAGAGGCCGG GGACTCGTGT CAGTGAGCGT CTTCGTGGAG CCCGGGCTGA GACTCAAGGG 9541 GCAAACCACA CTCCTGTCAT ATCCGCCCAT CAAACTCGCA GCACCACCAC ACCACCCCGC 9601 TGCAGTCCTG CCAGGGAGCG AGTTCCCAGG CCAGCACCTA GGCCTCGACC CACTCCAGCT 9661 TCAGCTCCGG CTGCAATTCC TGCCCTTGTT CCTGTCCCAG TTTCTGCCCC AGTACCCATT 9721 TCAGCCCCAA ATCCAATAAC CATTCTCCCT GTCCATATCT TGCCTTCTCC TCCCCCTCCT 9781 TCACAGATTC CTCCTTGTTC TTCTCCTGCC TGCACCCCTC CTCCTGCCTG TACCCCTCCA 9841 CCAGCTCATA CACCGCCTCC AGCCCAAACC TGTCTTGTAA CTCCTTCCTC TCCTCTCTTG 9901 CTTGGTCCAC CTTCTGTGCC CATCTCTGCC TCAGTCACTA ATCTCCCCTT GGGCTTGAGG 9961 CCTGAGGCAG AGCTGTGTGC CCAGGCATTG GCATCTCCAG AGTCCCTGGA GCTGGCTTCT 10021 GTGGCCAGTT CAGAAACCTC CTCACTTTCT CTTGTGCCCC CTAAAGATCT GTTGCCAGTT 10081 GCTGTGGAGA TCCTGCCTGT GTCAGAGAAG AACCTTTCTC TCACCCCTTC TGCACCCAGC 10141 CTGACCTTGG AGGCTGGCAG CATCCCCAAT GGTCAAGAGC AGGAGGCACC AGATTCTGCT 10201 GAGGGGACCA CCCTTACAGT GCTGCCTGAA GGTGAGGAGT TGCCCCTGTG TGTGAGTGAG 10261 AGCAATGGCC TGGAGCTCCC ACCCTCAGCA GCATCTGATG AGCCACTTCA GGAGCCACTG 10321 GAGGCTGACA GGACCTCGGA AGAGCTGACA GAGGCCAAGA CCCCAACCTC CAGCCCAGAG 10381 AAGCCACAGG AACTCGTTAC AGCTGAGGTT GCAGCTCCAT CCACCTCATC TTCAGCCACT 10441 TCCTCGCCTG AGGGTCCTTC ACCTGCCCGA CCTCCTCGGC GTCGCACCAG TGCTGATGTG 10501 GAAATTAGGG GTCAAGGGAC TGGTCGGCCA GGACAACCAC CAGGCCCCAA AGTGCTTCGA 10561 AAGCTGCCAG GACGGCTGGT AACTGTGGTA GAGGAAAAGG AACTGGTGCG GCGGCGGCGG 10621 CAGCAGCGGG GAGCTGCCAG CACCCTAGTG CCTGGGGTCT CTGAGACTAG TGCCAGCCCG 10681 GGAAGCCCGT CTGTCCGCAG CATGTCAGGG CCAGAATCCT CCCCTCCCAT TGGTGGGCCC 10741 TGTGAAGCTG CTCCTTCATC CTCACTGCCC ACTCCACCCC AGCAGCCCTT CATTGCTCGC 10801 CGTCACATTG AGCTGGGGGT GACTGGTGGT GGCAGCCCCG AGAATGGAGA CGGAGCACTG 10861 CTCGCCATCA CCCCACCTGC TGTGAAACGT CGGAGGGGGA GGCCCCCCAA GAAGAACAGG 10921 TCTCCAGCAG ATGCTGGGAG AGGTGTGGAT GAGGCACCCT CATCCACCTT GAAGGGAAAA 10981 ACCAATGGGG CTGACCCAGT CCCTGGGCCT GAGACCCTAA TTGTTGCAGA TCCTGTCCTG 11041 GAACCACAGC TTATTCCTGG GCCCCAGCCT CTTGGACCCC AGCCAGTTCA CAGACCCAAT 11101 CCCCTCCTGT CACCTGTGGA GAAAAGAAGG CGAGGACGAC CCCCTAAAGC ACGAGATTTG 11161 CCCATCCCTG GGACCATTTC CTCTGCAGGG GATGGCAACT CCGAAAGTCG GACACAGCCA 11221 CCCCCACACC CATCACCCCT AACCCCACTC CCACCACTGC TAGTTTGTCC CACTGCTACT 11281 GTTGCCAACA CTGTCACCAC TGTCACCATT TCAACGTCCC CACCCAAACG GAAGAGGGGC 11341 CGACCTCCCA AGAATCCTCC ATCACCTCGG CCCAGCCAGC TCCCCGTCTT GGACCGTGAC 11401 AGCACTTCTG TTCTCGAGAG CTGTGGATTG GGGAGGCGAC GGCAACCCCA GGGCCAAGGG 11461 GAGAGTGAGG GTAGTTCCTC TGATGAGGAT GGAAGCCGCC CCCTCACCCG CCTGGCCCGC 11521 CTTCGGCTTG AAGCAGAAGG AATGCGAGGA CGGAAGAGTG GAGGGTCCAT GGTGGTGGCT 11581 GTAATTCAGG ATGACCTGGA CTTAGCAGAT AGCGGGCCAG GCGGGTTGGA ATTGACACCA 11641 CCTGTGGTCT CACTAACCCC AAAACTGCGC TCGACCCGGC TGCGTCCAGG GTCTCTAGTC 11701 CCCCCACTAG AGACTGAGAA GTTGCCTCGC AAACGAGCAG GGGCCCCAGT TGGTGGGAGT 11761 CCTGGGCTGG CAAAGCGGGG CCGCCTACAG CCCCCAAGTC CCCTGGGGCC TGAGGGTTCA 11821 GTAGAGGAGT CTGAGGCTGA AGCCTCAGGT GAGGAGGAGG AAGGGGATGG GACCCCACGC 11881 CGACGTCCTG GCCCCCGCCG GCTTGTTGGG ACCACCAACC AAGGGGACCA GCGCATCCTG 11941 CGCAGCAGCG CCCCTCCCTC CCTGGCTGGC CCTGCTGTTA GTCACAGAGG CCGCAAGGCC 12001 AAGACGGGAT CCGACTACAA AGACCATGAC GGTGATTATA AAGATCATGA CATCGACTAC 12061 AAGGATGACG ATGACAAGTA GCGGCCGCGA AGGATCTGCG ATCGCTCCGG TGCCCGTCAG 12121 TGGGCAGAGC GCACATCGCC CACAGTCCCC GAGAAGTTGG GGGGAGGGGT CGGCAATTGA 12181 ACGGGTGCCT AGAGAAGGTG GCGCGGGGTA AACTGGGAAA GTGATGTCGT GTACTGGCTC 12241 CGCCTTTTTC CCGAGGGTGG GGGAGAACCG TATATAAGTG CAGTAGTCGC CGTGAACGTT 12301 CTTTTTCGCA ACGGGTTTGC CGCCAGAACA CAGCTGAAGC TTCGAGGGGC TCGCATCTCT 12361 CCTTCACGCG CCCGCCGCCC TACCTGAGGC CGCCATCCAC GCCGGTTGAG TCGCGTTCTG 12421 CCGCCTCCCG CCTGTGGTGC CTCCTGAACT GCGTCCGCCG TCTAGGTAAG TTTAAAGCTC 12481 AGGTCGAGAC CGGGCCTTTG TCCGGCGCTC CCTTGGAGCC TACCTAGACT CAGCCGGCTC 12541 TCCACGCTTT GCCTGACCCT GCTTGCTCAA CTCTACGTCT TTGTTTCGTT TTCTGTTCTG 12601 CGCCGTTACA GATCCAAGCT GTGACCGGCG CCTACGCTAG ACGCCACCAT GGAGAGCGAC 12661 GAGAGCGGCC TGCCCGCCAT GGAGATCGAG TGCCGCATCA CCGGCACCCT GAACGGCGTG 12721 GAGTTCGAGC TGGTGGGCGG CGGAGAGGGC ACCCCCAAGC AGGGCCGCAT GACCAACAAG 12781 ATGAAGAGCA CCAAAGGCGC CCTGACCTTC AGCCCCTACC TGCTGAGCCA CGTGATGGGC 12841 TACGGCTTCT ACCACTTCGG CACCTACCCC AGCGGCTACG AGAACCCCTT CCTGCACGCC 12901 ATCAACAACG GCGGCTACAC CAACACCCGC ATCGAGAAGT ACGAGGACGG CGGCGTGCTG 12961 CACGTGAGCT TCAGCTACCG CTACGAGGCC GGCCGCGTGA TCGGCGACTT CAAGGTGGTG 13021 GGCACCGGCT TCCCCGAGGA CAGCGTGATC TTCACCGACA AGATCATCCG CAGCAACGCC 13081 ACCGTGGAGC ACCTGCACCC CATGGGCGAT AACGTGCTGG TGGGCAGCTT CGCCCGCACC 13141 TTCAGCCTGC GCGACGGCGG CTACTACAGC TTCGTGGTGG ACAGCCACAT GCACTTCAAG 13201 AGCGCCATCC ACCCCAGCAT CCTGCAGAAC GGGGGCCCCA TGTTCGCCTT CCGCCGCGTG 13261 GAGGAGCTGC ACAGCAACAC CGAGCTGGGC ATCGTGGAGT ACCAGCACGC CTTCAAGACC 13321 CCCATCGCCT TCGCCAGATC CCGCGCTCAG TCGTCCAATT CTGCCGTGGA CGGCACCGCC 13381 GGACCCGGCT CCACCGGATC TCGCGAGGGC AGAGGAAGTC TTCTAACATG CGGTGACGTG 13441 GAGGAGAATC CCGGCCCTAT GACCGAGTAC AAGCCCACGG TGCGCCTCGC CACCCGCGAC 13501 GACGTCCCCA GGGCCGTACG CACCCTCGCC GCCGCGTTCG CCGACTACCC CGCCACGCGC 13561 CACACCGTCG ATCCGGACCG CCACATCGAG CGGGTCACCG AGCTGCAAGA ACTCTTCCTC 13621 ACGCGCGTCG GGCTCGACAT CGGCAAGGTG TGGGTCGCGG ACGACGGCGC CGCGGTGGCG 13681 GTCTGGACCA CGCCGGAGAG CGTCGAAGCG GGGGCGGTGT TCGCCGAGAT CGGCCCGCGC 13741 ATGGCCGAGT TGAGCGGTTC CCGGCTGGCC GCGCAGCAAC AGATGGAAGG CCTCCTGGCG 13801 CCGCACCGGC CCAAGGAGCC CGCGTGGTTC CTGGCCACCG TCGGCGTCTC GCCCGACCAC 13861 CAGGGCAAGG GTCTGGGCAG CGCCGTCGTG CTCCCCGGAG TGGAGGCGGC CGAGCGCGCC 13921 GGGGTGCCCG CCTTCCTGGA GACCTCCGCG CCCCGCAACC TCCCCTTCTA CGAGCGGCTC 13981 GGCTTCACCG TCACCGCCGA CGTCGAGGTG CCCGAAGGAC CGCGCACCTG GTGCATGACC 14041 CGCAAGCCCG GTGCCTGAAA TCAACCTCTG GATTACAAAA TTTGTGAAAG ATTGACTGGT 14101 ATTCTTAACT ATGTTGCTCC TTTTACGCTA TGTGGATACG CTGCTTTAAT GCCTTTGTAT 14161 CATGCTATTG CTTCCCGTAT GGCTTTCATT TTCTCCTCCT TGTATAAATC CTGGTTGCTG 14221 TCTCTTTATG AGGAGTTGTG GCCCGTTGTC AGGCAACGTG GCGTGGTGTG CACTGTGTTT 14281 GCTGACGCAA CCCCCACTGG TTGGGGCATT GCCACCACCT GTCAGCTCCT TTCCGGGACT 14341 TTCGCTTTCC CCCTCCCTAT TGCCACGGCG GAACTCATCG CCGCCTGCCT TGCCCGCTGC 14401 TGGACAGGGG CTCGGCTGTT GGGCACTGAC AATTCCGTGG TGTTGTCGGG GAAATCATCG 14461 TCCTTTCCTT GGCTGCTCGC CTGTGTTGCC ACCTGGATTC TGCGCGGGAC GTCCTTCTGC 14521 TACGTCCCTT CGGCCCTCAA TCCAGCGGAC CTTCCTTCCC GCGGCCTGCT GCCGGCTCTG 14581 CGGCCTCTTC CGCGTCTTCG CCTTCGCCCT CAGACGAGTC GGATCTCCCT TTGGGCCGCC 14641 TCCCCGCCTG GTACCTTTAA GACCAATGAC TTACAAGGCA GCTGTAGATC TTAGCCACTT 14701 TTTAAAAGAA AAGGGGGGAC TGGAAGGGCT AATTCACTCC CAACGAAGAT AAGATCTGCT 14761 TTTTGCTTGT ACTGGGTCTC TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA 14821 ACTAGGGAAC CCACTGCTTA AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG 14881 TGCCCGTCTG TTGTGTGACT CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG 14941 GAAAATCTCT AGCAGTAGTA GTTCATGTCA TCTTATTATT CAGTATTTAT AACTTGCAAA 15001 GAAATGAATA TCAGAGAGTG AGAGGAACTT GTTTATTGCA GCTTATAATG GTTACAAATA 15061 AAGCAATAGC ATCACAAATT TCACAAATAA AGCATTTTTT TCACTGCATT CTAGTTGTGG 15121 TTTGTCCAAA CTCATCAATG TATCTTATCA TGTCTGGCTC TAGCTATCCC GCCCCTAACT 15181 CCGCCCATCC CGCCCCTAAC TCCGCCCAGT TCCGCCCATT CTCCGCCCCA TGGCTGACTA 15241 ATTTTTTTTA TTTATGCAGA GGCCGAGGCC GCCTCGGCCT CTGAGCTATT CCAGAAGTAG 15301 TGAGGAGGCT TTTTTGGAGG CCTAGACTTT TGCAGAGACC AAATTCGTAA TCATGTCATA 15361 GCTGTTTCCT GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAC GAGCCGGAAG 15421 CATAAAGTGT AAAGCCTGGG GTGCCTAATG AGTGAGCTAA CTCACATTAA TTGCGTTGCG 15481 CTCACTGCCC GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT GAATCGGCCA 15541 ACGCGCGGGG AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC TCACTGACTC 15601 GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 15661 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 15721 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 15781 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 15841 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 15901 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 15961 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 16021 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 16081 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 16141 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGGAC 16201 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 16261 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 16321 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 16381 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 16441 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 16501 AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT 16561 ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG 16621 CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA 16681 TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT 16741 ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT 16801 TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT 16861 TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT 16921 GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC 16981 CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC 17041 CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT 17101 GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG 17161 AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT 17221 ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC 17281 TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA 17341 GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG 17401 AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA 17461 TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG TCTAAGAAAC 17521 CATTATTATC ATGACATTAA CCTATAAAAA TAGGCGTATC ACGAGGCCCT TTCGTCTCGC 17581 GCGTTTCGGT GATGACGGTG AAAACCTCTG ACACATGCAG CTCCCGGAGA CGGTCACAGC 17641 TTGTCTGTAA GCGGATGCCG GGAGCAGACA AGCCCGTCAG GGCGCGTCAG CGGGTGTTGG 17701 CGGGTGTCGG GGCTGGCTTA ACTATGCGGC ATCAGAGCAG ATTGTACTGA GAGTGCACCA 17761 TATGCGGTGT GAAATACCGC ACAGATGCGT AAGGAGAAAA TACCGCATCA GGCGCCATTC 17821 GCCATTCAGG CTGCGCAACT GTTGGGAAGG GCGATCGGTG CGGGCCTCTT CGCTATTACG 17881 CCAGCTGGCG AAAGGGGGAT GTGCTGCAAG GCGATTAAGT TGGGTAACGC CAGGGTTTTC 17941 CCAGTCACGA CGTTGTAAAA CGACGGCCAG TGCCAAGCTG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.