pCMV-NFATC2-3×Myc-Neo plasmid

pCMV-NFATC2-3×Myc-Neo plasmid

PVT86192

pCMV-NFATC2-3×Myc-Neo plasmid Information

Plasmid name: pCMV-NFATC2(human)-3×Myc-Neo
Alias: NM_012340.5;JCOSL; NFAT1; NFATP;
Gene lenght: 2763bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone: pCMV-MCS-3×Myc-Neo
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-NFATC2-3×Myc-Neo plasmid Description

pCMV-NFATC2-3×Myc-Neo This plasmid is suitable for overexpression in mammalian cells. The plasmid contains a CMV enhancer and CMV promoter that can jointly initiate the efficient expression of the downstream human NFATC2 gene, promoting the C-terminal fusion expression of a 3×Myc tag for easy identification and tracking of the Myc-tagged target protein in experiments such as Western blotting, immunoprecipitation, or immunofluorescence, thereby studying the expression level, subcellular localization, and interactions with other proteins of the target protein. The plasmid carries a Neo/G418 resistance gene for selecting host cells that express the target gene.

pCMV-NFATC2-3×Myc-Neo plasmid Sequence

1 GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA 61 TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG 121 TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC 181 CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA 241 CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT 301 ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC 361 CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT 421 ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA 481 CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT 541 CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG 601 CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTGGCTA GCGTTTAAAC TTAAGCTTGG 661 TACCGAGCTC GGATCCACTA GTCCAGTGTG GTGGAATTCG CCACCATGAA CGCCCCCGAG 721 CGGCAGCCCC AACCCGACGG CGGGGACGCC CCAGGCCACG AGCCTGGGGG CAGCCCCCAA 781 GACGAGCTTG ACTTCTCCAT CCTCTTCGAC TATGAGTATT TGAATCCGAA CGAAGAAGAG 841 CCGAATGCAC ATAAGGTCGC CAGCCCACCC TCCGGACCCG CATACCCCGA TGATGTCCTG 901 GACTATGGCC TCAAGCCATA CAGCCCCCTT GCTAGTCTCT CTGGCGAGCC CCCCGGCCGA 961 TTCGGAGAGC CGGATAGGGT AGGGCCGCAG AAGTTTCTGA GCGCGGCCAA GCCAGCAGGG 1021 GCCTCGGGCC TGAGCCCTCG GATCGAGATC ACTCCGTCCC ACGAACTGAT CCAGGCAGTG 1081 GGGCCCCTCC GCATGAGAGA CGCGGGCCTC CTGGTGGAGC AGCCGCCCCT GGCCGGGGTG 1141 GCCGCCAGCC CGAGGTTCAC CCTGCCCGTG CCCGGCTTCG AGGGCTACCG CGAGCCGCTT 1201 TGCTTGAGCC CCGCTAGCAG CGGCTCCTCT GCCAGCTTCA TTTCTGACAC CTTCTCCCCC 1261 TACACCTCGC CCTGCGTCTC GCCCAATAAC GGCGGGCCCG ACGACCTGTG TCCGCAGTTT 1321 CAAAACATCC CTGCTCATTA TTCCCCCAGA ACCTCGCCAA TAATGTCACC TCGAACCAGC 1381 CTCGCCGAGG ACAGCTGCCT GGGCCGCCAC TCGCCCGTGC CCCGTCCGGC CTCCCGCTCC 1441 TCATCGCCTG GTGCCAAGCG GAGGCATTCG TGCGCCGAGG CCTTGGTTGC CCTGCCGCCC 1501 GGAGCCTCAC CCCAGCGCTC CCGGAGCCCC TCGCCGCAGC CCTCATCTCA CGTGGCACCC 1561 CAGGACCACG GCTCCCCGGC TGGGTACCCC CCTGTGGCTG GCTCTGCCGT GATCATGGAT 1621 GCCCTGAACA GCCTCGCCAC GGACTCGCCT TGTGGGATCC CCCCCAAGAT GTGGAAGACC 1681 AGCCCTGACC CCTCGCCGGT GTCTGCCGCC CCATCCAAGG CCGGCCTGCC TCGCCACATC 1741 TACCCGGCCG TGGAGTTCCT GGGGCCCTGC GAGCAGGGCG AGAGGAGAAA CTCGGCTCCA 1801 GAATCCATCC TGCTGGTTCC GCCCACTTGG CCCAAGCCGC TGGTGCCTGC CATTCCCATC 1861 TGCAGCATCC CAGTGACTGC ATCCCTCCCT CCACTTGAGT GGCCGCTGTC CAGTCAGTCA 1921 GGCTCTTACG AGCTGCGGAT CGAGGTGCAG CCCAAGCCAC ATCACCGGGC CCACTATGAG 1981 ACAGAAGGCA GCCGAGGGGC TGTCAAAGCT CCAACTGGAG GCCACCCTGT GGTTCAGCTC 2041 CATGGCTACA TGGAAAACAA GCCTCTGGGA CTTCAGATCT TCATTGGGAC AGCTGATGAG 2101 CGGATCCTTA AGCCGCACGC CTTCTACCAG GTGCACCGAA TCACGGGGAA AACTGTCACC 2161 ACCACCAGCT ATGAGAAGAT AGTGGGCAAC ACCAAAGTCC TGGAGATACC CTTGGAGCCC 2221 AAAAACAACA TGAGGGCAAC CATCGACTGT GCGGGGATCT TGAAGCTTAG AAACGCCGAC 2281 ATTGAGCTGC GGAAAGGCGA GACGGACATT GGAAGAAAGA ACACGCGGGT GAGACTGGTT 2341 TTCCGAGTTC ACATCCCAGA GTCCAGTGGC AGAATCGTCT CTTTACAGAC TGCATCTAAC 2401 CCCATCGAGT GCTCCCAGCG ATCTGCTCAC GAGCTGCCCA TGGTTGAAAG ACAAGACACA 2461 GACAGCTGCC TGGTCTATGG CGGCCAGCAA ATGATCCTCA CGGGGCAGAA CTTTACATCC 2521 GAGTCCAAAG TTGTGTTTAC TGAGAAGACC ACAGATGGAC AGCAAATTTG GGAGATGGAA 2581 GCCACGGTGG ATAAGGACAA GAGCCAGCCC AACATGCTTT TTGTTGAGAT CCCTGAATAT 2641 CGGAACAAGC ATATCCGCAC ACCTGTAAAA GTGAACTTCT ACGTCATCAA TGGGAAGAGA 2701 AAACGAAGTC AGCCTCAGCA CTTTACCTAC CACCCAGTCC CAGCCATCAA GACGGAGCCC 2761 ACGGATGAAT ATGACCCCAC TCTGATCTGC AGCCCCACCC ATGGAGGCCT GGGGAGCCAG 2821 CCTTACTACC CCCAGCACCC GATGGTGGCC GAGTCCCCCT CCTGCCTCGT GGCCACCATG 2881 GCTCCCTGCC AGCAGTTCCG CACGGGGCTC TCATCCCCTG ACGCCCGCTA CCAGCAACAG 2941 AACCCAGCGG CCGTACTCTA CCAGCGGAGC AAGAGCCTGA GCCCCAGCCT GCTGGGCTAT 3001 CAGCAGCCGG CCCTCATGGC CGCCCCGCTG TCCCTTGCGG ACGCTCACCG CTCTGTGCTG 3061 GTGCACGCCG GCTCCCAGGG CCAGAGCTCA GCCCTGCTCC ACCCCTCTCC GACCAACCAG 3121 CAGGCCTCGC CTGTGATCCA CTACTCACCC ACCAACCAGC AGCTGCGCTG CGGAAGCCAC 3181 CAGGAGTTCC AGCACATCAT GTACTGCGAG AATTTCGCAC CAGGCACCAC CAGACCTGGC 3241 CCGCCCCCGG TCAGTCAAGG TCAGAGGCTG AGCCCGGGTT CCTACCCCAC AGTCATTCAG 3301 CAGCAGAATG CCACGAGCCA AAGAGCCGCC AAAAACGGAC CCCCGGTCAG TGACCAAAAG 3361 GAAGTATTAC CTGCGGGGGT GACCATTAAA CAGGAGCAGA ACTTGGACCA GACCTACTTG 3421 GATGATGAGC TGATAGACAC ACACCTTAGC TGGATACAAA ACATATTACT CGAGGAACAA 3481 AAACTAATAT CAGAGGAAGA TTTGGAACAG AAACTAATCA GTGAAGAAGA TTTAGAGCAA 3541 AAGTTAATTT CCGAGGAGGA CTTATAGTGA GGGCCCGATA TCTCGACAAT CAACCTCTGG 3601 ATTACAAAAT TTGTGAAAGA TTGACTGGTA TTCTTAACTA TGTTGCTCCT TTTACGCTAT 3661 GTGGATACGC TGCTTTAATG CCTTTGTATC ATGCTATTGC TTCCCGTATG GCTTTCATTT 3721 TCTCCTCCTT GTATAAATCC TGGTTGCTGT CTCTTTATGA GGAGTTGTGG CCCGTTGTCA 3781 GGCAACGTGG CGTGGTGTGC ACTGTGTTTG CTGACGCAAC CCCCACTGGT TGGGGCATTG 3841 CCACCACCTG TCAGCTCCTT TCCGGGACTT TCGCTTTCCC CCTCCCTATT GCCACGGCGG 3901 AACTCATCGC CGCCTGCCTT GCCCGCTGCT GGACAGGGGC TCGGCTGTTG GGCACTGACA 3961 ATTCCGTGGT GTTGTCGGGG AAGCTGACGT CCTTTCCATG GCTGCTCGCC TGTGTTGCCA 4021 CCTGGATTCT GCGCGGGACG TCCTTCTGCT ACGTCCCTTC GGCCCTCAAT CCAGCGGACC 4081 TTCCTTCCCG CGGCCTGCTG CCGGCTCTGC GGCCTCTTCC GCGTCTTCGC CTTCGCCCTC 4141 AGACGAGTCG GATCTCCCTT TGGGCCGCCT CCCCGCCTGG AAACGGGGGA GGCTAACTGA 4201 AACACGGAAG GAGACAATAC CGGAAGGAAC CCGCGCTATG ACGGCAATAA AAAGACAGAA 4261 TAAAACGCAC GGGTGTTGGG TCGTTTGTTC ATAAACGCGG GGTTCGGTCC CAGGGCTGGC 4321 ACTCTGTCGA TACCCCACCG AGACCCCATT GGGGCCAATA CGCCCGCGTT TCTTCCTTTT 4381 CCCCACCCCA CCCCCCAAGT TCGGGTGAAG GCCCAGGGCT CGCAGCCAAC GTCGGGGCGG 4441 CAGGCCCTGC CATAGCAGAT CTGCGCAGCT GGGGCTCTAG GGGGTATCCC CACGCGCCCT 4501 GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG 4561 CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG 4621 GCTTTCCCCG TCAAGCTCTA AATCGGGGCA TCCCTTTAGG GTTCCGATTT AGTGCTTTAC 4681 GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT 4741 GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT 4801 TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT 4861 TGGGGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT 4921 AATTCTGTGG AATGTGTGTC AGTTAGGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG 4981 AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCAGG TGTGGAAAGT CCCCAGGCTC 5041 CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA TAGTCCCGCC 5101 CCTAACTCCG CCCATCCCGC CCCTAACTCC GCCCAGTTCC GCCCATTCTC CGCCCCATGG 5161 CTGACTAATT TTTTTTATTT ATGCAGAGGC CGAGGCCGCC TCTGCCTCTG AGCTATTCCA 5221 GAAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC AAAAAGCTCC CGGGAGCTTG 5281 TATATCCATT TTCGGATCTG ATCAAGAGAC AGGATGAGGA TCGTTTCGCA TGATTGAACA 5341 AGATGGATTG CACGCAGGTT CTCCGGCCGC TTGGGTGGAG AGGCTATTCG GCTATGACTG 5401 GGCACAACAG ACAATCGGCT GCTCTGATGC CGCCGTGTTC CGGCTGTCAG CGCAGGGGCG 5461 CCCGGTTCTT TTTGTCAAGA CCGACCTGTC CGGTGCCCTG AATGAACTGC AGGACGAGGC 5521 AGCGCGGCTA TCGTGGCTGG CCACGACGGG CGTTCCTTGC GCAGCTGTGC TCGACGTTGT 5581 CACTGAAGCG GGAAGGGACT GGCTGCTATT GGGCGAAGTG CCGGGGCAGG ATCTCCTGTC 5641 ATCTCACCTT GCTCCTGCCG AGAAAGTATC CATCATGGCT GATGCAATGC GGCGGCTGCA 5701 TACGCTTGAT CCGGCTACCT GCCCATTCGA CCACCAAGCG AAACATCGCA TCGAGCGAGC 5761 ACGTACTCGG ATGGAAGCCG GTCTTGTCGA TCAGGATGAT CTGGACGAAG AGCATCAGGG 5821 GCTCGCGCCA GCCGAACTGT TCGCCAGGCT CAAGGCGCGC ATGCCCGACG GCGAGGATCT 5881 CGTCGTGACC CATGGCGATG CCTGCTTGCC GAATATCATG GTGGAAAATG GCCGCTTTTC 5941 TGGATTCATC GACTGTGGCC GGCTGGGTGT GGCGGACCGC TATCAGGACA TAGCGTTGGC 6001 TACCCGTGAT ATTGCTGAAG AGCTTGGCGG CGAATGGGCT GACCGCTTCC TCGTGCTTTA 6061 CGGTATCGCC GCTCCCGATT CGCAGCGCAT CGCCTTCTAT CGCCTTCTTG ACGAGTTCTT 6121 CTGAGCGGGA CTCTGGGGTT CGCGAAATGA CCGACCAAGC GACGCCCAAC CTGCCATCAC 6181 GAGATTTCGA TTCCACCGCC GCCTTCTATG AAAGGTTGGG CTTCGGAATC GTTTTCCGGG 6241 ACGCCGGCTG GATGATCCTC CAGCGCGGGG ATCTCATGCT GGAGTTCTTC GCCCACCCCA 6301 ACTTGTTTAT TGCAGCTTAT AATGGTTACA AATAAAGCAA TAGCATCACA AATTTCACAA 6361 ATAAAGCATT TTTTTCACTG CATTCTAGTT GTGGTTTGTC CAAACTCATC AATGTATCTT 6421 ATCATGTCTG TATACCGTCG ACCTCTAGCT AGAGCTTGGC GTAATCATGG TCATAGCTGT 6481 TTCCTGTGTG AAATTGTTAT CCGCTCACAA TTCCACACAA CATACGAGCC GGAAGCATAA 6541 AGTGTAAAGC CTGGGGTGCC TAATGAGTGA GCTAACTCAC ATTAATTGCG TTGCGCTCAC 6601 TGCCCGCTTT CCAGTCGGGA AACCTGTCGT GCCAGCTGCA TTAATGAATC GGCCAACGCG 6661 CGGGGAGAGG CGGTTTGCGT ATTGGGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC 6721 GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT 6781 CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA 6841 GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC 6901 ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC 6961 AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG 7021 GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCAATGC TCACGCTGTA 7081 GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG 7141 TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC 7201 ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG 7261 GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGGACAGTAT 7321 TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT 7381 CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC 7441 GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT 7501 GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT 7561 AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT 7621 GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC 7681 GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC 7741 CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGACCCACG CTCACCGGCT CCAGATTTAT 7801 CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG 7861 CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA 7921 GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA 7981 TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT 8041 GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG 8101 TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA 8161 GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC 8221 GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT 8281 TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC 8341 TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA 8401 CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA 8461 TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA 8521 TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC 8581 AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGTCGAC GGATCGGGAG 8641 ATCTCCCGAT CCCCTATGGT CGACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG 8701 CCAGTATCTG CTCCCTGCTT GTGTGTTGGA GGTCGCTGAG TAGTGCGCGA GCAAAATTTA 8761 AGCTACAACA AGGCAAGGCT TGACCGACAA TTGCATGAAG AATCTGCTTA GG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.