pAR18_StrepI_mFASm_H8_pET22b p

pAR18_StrepI_mFASm_H8_pET22b plasmid

PVT85757

pAR18_StrepI_mFASm_H8_pET22b plasmid Information

Plasmid name: pAR18_StrepI_mFASm_H8_pET22b
Alias: Plasmid#122847
Gene lenght: 7647bp
Host: E.coli
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling:
Promoter: T7
Replicon: pBR322
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone: pET22b
Forward primer: T7
Reverse primer:
Copy number: High
Induction: IPTG Induction

pAR18_StrepI_mFASm_H8_pET22b plasmid Description

pAR18_StrepI_mFASm_H8_pET22b It is a prokaryotic induction-type expression plasmid. The plasmid has a T7-lacO promoter system, under the induction of IPTG or lactose and its analogs, T7 RNA polymerase initiates the transcription and translation of the mouse FASN fatty acid synthase gene. This plasmid carries a Twin-Strep tag at the N-terminus of the FASN gene and an 8×His tag at the C-terminus of the FASN gene, which facilitates subsequent detection, purification, and localization studies of the expressed protein.

pAR18_StrepI_mFASm_H8_pET22b plasmid Sequence

1 TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC 61 GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT 121 GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC 181 AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT 241 CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGGAAT TGTGAGCGGA TAACAATTCC 301 CCTCTAGAAA TAATTTTGTT TAACTTTAAG AAGGAGATAT ACATATGAGC GCTTGGAGCC 361 ATCCACAATT TGAGAAGGGT GGAGGTTCTG GCGGTGGATC GGGAGGTTCA GCGTGGAGCC 421 ACCCGCAGTT CGAAAAAGGC GCCGGATCCG AGGAGGTGGT GATAGCCGGT ATGTCGGGGA 481 AGTTGCCCGA GTCAGAGAAC CTACAGGAGT TCTGGGCCAA CCTCATTGGT GGTGTGGACA 541 TGGTCACAGA TGATGACAGG AGATGGAAGG CTGGGCTCTA TGGATTACCC AAGCGGTCTG 601 GAAAGCTGAA GGATCTCTCC AAGTTCGACG CCTCCTTTTT TGGGGTCCAC CCCAAGCAGG 661 CACACACAAT GGACCCCCAG CTTCGGCTGC TGTTGGAAGT CAGCTATGAA GCAATTGTGG 721 ATGGAGGTAT CAACCCAGCC TCACTCCGAG GAACGAACAC TGGCGTCTGG GTGGGTGTGA 781 GTGGTTCAGA GGCATCCGAG GCCCTTAGCA GAGATCCCGA GACGCTTCTG GGCTACAGCA 841 TGGTGGGCTG CCAGCGTGCA ATGATGGCCA ACCGGCTCTC TTTCTTCTTC GACTTCAAAG 901 GACCAAGCAT TGCCCTGGAC ACAGCCTGCT CCTCCAGCTT GCTGGCACTA CAGAATGCCT 961 ACCAGGCCAT CCGTAGTGGG GAATGCCCCG CGGCCCTTGT GGGTGGGATC AACCTGCTCC 1021 TGAAGCCGAA CACCTCTGTG CAGTTCATGA AGCTGGGCAT GCTCAGCCCG GACGGCACCT 1081 GCAGATCCTT TGATGATTCA GGGAGTGGAT ATTGTCGCTC TGAGGCTGTT GTAGCAGTTC 1141 TGCTGACTAA GAAGTCCCTG GCTCGGCGGG TCTATGCCAC GATTCTGAAT GCCGGCACCA 1201 ATACAGATGG CAGCAAGGAG CAAGGTGTAA CATTCCCCTC TGGAGAAGTC CAAGAACAAC 1261 TCATCTGCTC TCTGTATCAG CCAGCTGGTC TGGCCCCGGA GTCGCTTGAG TATATTGAAG 1321 CCCATGGCAC GGGCACCAAG GTGGGTGACC CCCAGGAACT GAATGGCATT ACTCGGTCCC 1381 TGTGCGCCTT CCGCCAGGCC CCTCTGTTAA TTGGCTCCAC CAAATCCAAC ATGGGACACC 1441 CTGAGCCTGC CTCTGGGCTT GCAGCCCTGA CCAAGGTGCT GTTATCCCTG GAGCATGGGG 1501 TCTGGGCCCC TAACCTGCAC TTCCACAACC CCAACCCTGA GATCCCAGCA CTTCTTGATG 1561 GGCGGCTGCA GGTGGTCGAT AGGCCCCTGC CTGTTCGTGG TGGCAACGTG GGCATCAACT 1621 CATTTGGCTT CGGAGGCTCC AATGTTCATG TCATCCTCCA GCCCAACACA CGGCAGGCCC 1681 CTGCGCCCAC TGCACACGCT GCCCTTCCCC ATTTGCTGCA CGCCAGTGGA CGCACCTTAG 1741 AGGCAGTGCA GGACCTGCTG GAACAGGGCC GCCAGCACAG CCAGGACCTG GCCTTTGTGA 1801 GCATGCTCAA TGACATTGCG GCAACCCCTA CAGCAGCCAT GCCCTTCAGG GGTTACACTG 1861 TGCTAGGTGT TGAGGGCCGT GTCCAAGAAG TGCAGCAAGT GTCCACCAAC AAGCGCCCAC 1921 TCTGGTTCAT CTGCTCAGGG ATGGGCACGC AGTGGCGCGG GATGGGGCTG AGCCTCATGC 1981 GCCTGGACAG CTTCCGTGAG TCTATCCTGC GCTCCGATGA GGCTGTGAAG CCGTTGGGAG 2041 TGAAAGTGTC AGATCTGCTG TTGAGCACAG ATGAGCGCAC CTTTGATGAC ATCGTGCATG 2101 CCTTTGTGAG CCTCACTGCC ATCCAGATTG CCCTCATCGA CCTACTGACT TCTGTGGGAC 2161 TGAAACCTGA CGGCATCATT GGGCACTCCT TGGGAGAGGT TGCCTGTGGC TATGCAGATG 2221 GCTGTCTCTC CCAGAGAGAG GCTGTGCTTG CAGCTTACTG GCGAGGCCAG TGCATCAAAG 2281 ATGCCCACCT CCCGCCTGGA TCCATGGCAG CTGTTGGTTT GTCCTGGGAG GAATGTAAAC 2341 AGCGCTGCCC CGCTGGCGTG GTGCCTGCCT GCCACAACTC TGAGGACACC GTGACCATCT 2401 CTGGACCTCA GGCTGCAGTG AATGAATTTG TGGAGCAGCT AAAGCAAGAA GGTGTGTTTG 2461 CCAAGGAGGT ACGAACAGGA GGCCTGGCTT TCCACTCCTA CTTCATGGAA GGAATTGCCC 2521 CCACATTGCT GCAGGCTCTC AAGAAGGTGA TCCGGGAACC ACGGCCGCGC TCGGCTCGAT 2581 GGCTCAGCAC CTCTATCCCT GAGGCCCAGT GGCAGAGCAG CCTGGCCCGC ACATCTTCTG 2641 CCGAGTACAA TGTCAACAAC CTGGTGAGCC CTGTGCTCTT CCAGGAAGCA CTGTGGCACA 2701 TCCCTGAGCA TGCCGTGGTG CTGGAGATTG CGCCCCACGC ACTGTTGCAG GCTGTCCTGA 2761 AGCGAGGCGT GAAGTCCAGC TGCACCATCA TTCCCTTGAT GAAGAGGGAT CATAAAGATA 2821 ACTTGGAGTT CTTTCTCACC AACCTTGGCA AGGTGCACCT CACAGGCATC AATGTCAACC 2881 CTAACGCCTT GTTCCCACCT GTGGAGTTCC CGGCTCCCCG AGGGACTCCT CTCATCTCCC 2941 CTCACATCAA GTGGGACCAC AGTCAGACTT GGGATGTCCC GGTTGCTGAG GACTTCCCAA 3001 ACGGCTCCAG CTCCTCCTCT GCTACAGTCT ACAGCATCGA CGCCAGTCCT GAGTCGCCCG 3061 ACCACTACCT GGTAGACCAC TGCATTGACG GCCGGGTCAT CTTCCCTGGC ACTGGCTACC 3121 TGTGCCTGGT GTGGAAGACA CTGGCTCGCA GCCTGGGCTT GTCCCTAGAA GAGACCCCTG 3181 TGGTATTTGA GAATGTGTCG TTTCATCAGG CCACTATACT ACCCAAGACA GGAACCGTGG 3241 CGCTGGAGGT GAGGCTGCTA GAGGCCTCCC ATGCCTTTGA GGTGTCTGAC ACTGGCAATC 3301 TGATTGTGAG CGGAAAAGTG TACCTGTGGG AAGACCCGAA CTCCAAGTTA TTCGACCACC 3361 CAGAAGTCCC AACACCCCCT GAGTCTGCAT CGGTCTCCCG CCTGACCCAG GGAGAAGTAT 3421 ACAAGGAGCT GCGGCTGCGT GGCTATGATT ATGGCCCTCA GTTCCAGGGC ATCTGTGAGG 3481 CCACCCTTGA AGGTGAACAA GGCAAGCTGC TCTGGAAAGA TAACTGGGTG ACCTTCATGG 3541 ACACAATGCT GCAGGTATCC ATTCTGGGTT CTAGCCAGCA GAGTCTACAG CTACCTACCC 3601 GTGTGACCGC CATCTATATC GACCCTGCCA CCCACCGTCA GAAGGTGTAC AGGCTGAAGG 3661 AGGACACTCA AGTGGCTGAT GTGACAACGA GCCGCTGTCT GGGCATAACG GTCTCTGGTG 3721 GTATCCACAT CTCAAGACTA CAGACGACAG CAACCTCACG GCGGCAGCAA GAACAGCTGG 3781 TCCCCACCTT GGAAAAGTTC GTTTTCACAC CGCACATGGA GGCTGAGTGC CTGTCTGAGA 3841 GCACTGCCCT GCAGAAGGAG CTGCAACTGT GCAAGGGTCT GGCACGGGCT CTGCAGACCA 3901 AGGCCACCCA GCAAGGGCTG AAGGCGGCAA TGCTTGGGCA AGAGGACCCT CCACAGCACG 3961 GGCTGCCTCG ACTCCTGGCA GCTGCTTGCC AGTTGCAGCT CAACGGGAAC CTGCAGCTGG 4021 AGCTGGGAGA AGCGCTGGCT CAAGAGAGGC TCCTGCTGCC AGAAGACCCT CTGATCAGTG 4081 GCCTCCTCAA CTCCCAGGCC CTCAAGGCCT GCGTAGACAC AGCCCTGGAG AACTTGTCTA 4141 CTCTCAAGAT GAAGGTGGCA GAGGTGCTGG CTGGAGAAGG CCACTTGTAT TCCCGAATCC 4201 CGGCACTGCT CAACACCCAG CCCATGCTAC AACTGGAATA CACAGCCACC GACCGGCACC 4261 CCCAGGCCCT GAAGGATGTT CAGACCAAAC TGCAGCAGCA TGATGTGGCG CAGGGCCAGT 4321 GGAACCCTTC CGACCCTGCG CCCAGCAGCC TGGGTGCCCT TGACCTTCTG GTGTGCAACT 4381 GTGCATTAGC CACCCTGGGG GATCCAGCCT TGGCCCTGGA CAACATGGTA GCTGCCCTCA 4441 AGGAAGGTGG TTTCCTGCTA GTGCACACAG TGCTCAAAGG ACATGCCCTT GGGGAGACCC 4501 TGGCCTGCCT ACCCTCTGAG GTGCAGCCTG CGCCCAGCCT CCTAAGCCAG GAGGAGTGGG 4561 AGAGCCTGTT CTCGAGGAAG GCACTACACC TGGTGGGCCT TAAAAGGTCC TTCTACGGTA 4621 CTGCGCTGTT CCTGTGCCGG CGAGCCATCC CACAGGAGAA ACCTATCTTC CTGTCTGTGG 4681 AGGATACCAG CTTCCAGTGG GTGGACTCTC TGAAGAGCAC TCTGGCCACG TCCTCCTCCC 4741 AGCCTGTGTG GCTAACGGCC ATGGACTGCC CCACCTCGGG TGTGGTGGGT TTGGTGAATT 4801 GTCTCCGAAA AGAGCCGGGT GGACACCGGA TTCGGTGTAT CCTGCTGTCC AACCTCAGCA 4861 ACACATCTCA CGCCCCCAAG TTGGACCCTG GCTCTCCAGA GCTACAGCAG GTGCTAAAGC 4921 ATGACCTCGT GATGAACGTG TACCGGGACG GGGCCTGGGG TGCCTTCCGT CACTTCCAGT 4981 TAGAGCAGGA CAAGCCCAAG GAGCAGACAG CGCATGCCTT TGTAAACGTC CTCACCCGAG 5041 GGGACCTCGC CTCCATCCGC TGGGTCTCCT CCCCCCTGAA GCACACGCAG CCCTCGAGCT 5101 CAGGAGCACA GCTCTGCACT GTCTACTACG CCTCACTGAA CTTCCGAGAC ATCATGCTGG 5161 CCACGGGCAA GCTGTCCCCT GATGCCATTC CAGGTAAATG GGCCAGCCGA GACTGCATGC 5221 TCGGCATGGA GTTCTCAGGC CGGGATAGGT GTGGCCGGCG TGTGATGGGG CTGGTTCCTG 5281 CAGAAGGCCT GGCCACCTCA GTCCTGCTAT CATCTGACTT CCTCTGGGAT GTACCCTCCA 5341 GCTGGACCCT GGAGGAGGCG GCCTCTGTGC CCGTCGTCTA TACCACTGCT TACTACTCGT 5401 TAGTGGTTCG CGGGCGCATC CAGCGTGGGG AGACCGTGCT CATCCACTCA GGTTCAGGTG 5461 GTGTGGGCCA AGCGGCCATT TCCATTGCCC TCAGTCTGGG CTGCCGCGTC TTCACCACTG 5521 TGGGCTCTGC AGAGAAGCGA GCATACCTCC AGGCCAGGTT CCCTCAGCTT GATGACACCA 5581 GCTTTGCCAA CTCGAGGGAC ACATCATTTG AGCAGCACGT GTTACTGCAC ACAGGTGGCA 5641 AAGGGGTCGA CCTGGTCCTC AACTCACTGG CAGAAGAGAA GCTGCAGGCC AGTGTGCGGT 5701 GCTTGGCTCA GCATGGTCGC TTCTTAGAGA TTGGCAAATT TGATCTTTCT AACAACCACC 5761 CTCTGGGCAT GGCTATCTTC TTGAAGAACG TCACTTTCCA TGGGATCCTG CTGGACGCCC 5821 TTTTTGAGGA GGCCAATGAC AGCTGGCGGG AGGTGGCGGC ACTCCTGAAG GCTGGCATTC 5881 GTGATGGAGT CGTGAAGCCC CTCAAGTGCA CAGTGTTTCC CAAGGCCCAG GTGGAAGATG 5941 CCTTCCGCTA CATGGCTCAG GGGAAACACA TTGGCAAAGT CCTTGTCCAG GTACGGGAGG 6001 AGGAGCCTGA GGCTGTGCTG CCAGGGGCTC AGCCCACCCT GATTTCTGCC ATCTCCAAGA 6061 CCTTCTGCCC AGCCCATAAG AGTTACATCA TCACTGGTGG CCTAGGTGGC TTTGGCCTGG 6121 AGCTGGCCCG GTGGCTCGTG CTTCGCGGAG CCCAGAGGCT TGTGCTGACT TCCCGATCTG 6181 GAATCCGCAC CGGCTACCAA GCCAAGCACA TTCGGGAGTG GAGACGCCAG GGCATCCAAG 6241 TGCTCGTGTC AACAAGCAAC GTGAGCTCAC TGGAGGGGGC CCGTGCTCTC ATCGCCGAAG 6301 CCACAAAGCT GGGGCCCGTT GGGGGTGTCT TCAACCTGGC CATGGTTTTG AGGGATGCCA 6361 TGCTGGAGAA CCAGACCCCA GAGCTCTTCC AGGATGTCAA CAAGCCCAAA TACAATGGCA 6421 CCCTGAACCT TGACAGGGCA ACCCGGGAAG CCTGCCCTGA GCTGGACTAC TTTGTGGCCT 6481 TCTCCTCTGT AAGCTGCGGG CGTGGTAATG CTGGCCAAAC TAACTACGGC TTCGCCAACT 6541 CTACCATGGA GCGTATATGT GAACAGCGCA GGCACGATGG CCTCCCAGGC CTTGCCGTGC 6601 AGTGGGGTGC CATTGGTGAC GTGGGCATTG TCCTGGAAGC GATGGGCACC AATGACACAG 6661 TCATCGGAGG TACGCTGCCT CAGCGCATCT CCTCCTGCAT GGAGGTACTG GACCTCTTCC 6721 TGAATCAGCC CCACGCAGTC CTGAGCAGCT TTGTGCTGGC AGAGAAGAAA GCTGTGGCCC 6781 ATGGGGACGG GGACACCCAG AGGGATCTGG TGAAAGCTGT AGCACACATC CTAGGCATCC 6841 GAGACCTCGC AGGTATTAAC CTGGACAGCA CGCTGGCAGA CCTCGGCCTG GACTCGCTCA 6901 TGGGTGTGGA AGTTCGTCAG ATCCTGGAAC GAGAACACGA TCTGGTGCTG CCCATGCGTG 6961 AGGTGCGGCA GCTCACGCTG CGGAAACTTC AGGAAATGTC CTCCAAGACT GACTCGGCTA 7021 CTGACACGAC AGCCCCCAAG TCCAGGAGTG ACACGTCTCT GAAGCAGAAC CAACTGAACC 7081 TGAGCACACT GCTGGTGAAC CCTGAGGGTC CTACCCTAAC CCAGCTCAAC TCGGTGCAGA 7141 GCTCTGAGCG GCCTCTGTTC CTTGTGCACC CCATTGAGGG TTCCACCACC GTGTTCCACA 7201 GTCTGGCTGC CAAGCTCAGT GTGCCCACCT ACGGCCTGCA GTGCACCCAA GCTGCCCCCC 7261 TGGATAGCAT TCCGAACCTG GCTGCCTACT ACATAGATTG CATCAAGCAA GTGCAGCCTG 7321 AGGGACCCTA CCGCATAGCT GGGTACTCAT TTGGAGCCTG TGTAGCCTTC GAGATGTGCT 7381 CCCAGCTGCA GGCCCAGCAG GGCCCAGCCC CGACCCACAA CAACCTCTTC CTGTTTGACG 7441 GCTCACACAC CTACGTGTTG GCCTACACCC AGAGCTACCG GGCAAAGATG ACCCCAGGCT 7501 GTGAAGCCGA GGCCGAGGCT GAGGCCTTAT GCTTCTTCAT AAAGCAGTTT CTTGATGTGG 7561 AACACAGCAA GGTGCTGGAG GCCCTGCTGC CACTGAAGAG CCTGGAAGAT CGGGTGGCTG 7621 CCTCCGTGGA CCTTATCACT AAGAGTCACC ACAGCCTGGA CCGCCGAGAG CTGAGCTTTG 7681 CTGCCGTGTC CTTCTACCAC AAGCTCCGGG CAGCTGATCA GTATAAGCCC AAGGCCAAGT 7741 ACCATGGCAA CGTGACACTG CTGCGTGCCA AGACAGGCGG CACCTATGGC GAGGACTTGG 7801 GTGCTGACTA CAACCTCTCC CAGGTGTGTG ACGGGAAGGT GTCTGTGCAC ATCATTGAGG 7861 GTGACCACCG CACACTGCTG GAGGGCAGTG GCCTGGAATC CATCATCAAC ATCATCCATA 7921 GCTCCCTGGC TGAGCCACGA GTGAGTGTAC GGGAGGGCCT CGAGCATCAT CACCACCACC 7981 ACCACCACTG AGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA 8041 CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT 8101 TGCTGAAAGG AGGAACTATA TCCGGATTGG CGAATGGGAC GCGCCCTGTA GCGGCGCATT 8161 AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC 8221 GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA 8281 AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC 8341 CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT 8401 TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC 8461 AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC 8521 CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTTA ACAAAATATT 8581 AACGCTTACA ATTTAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA 8641 TTTTTCTAAA TACATTCAAA TATGTATTCG CTCATGAGAC AATAACCCTG ATAAATGCTT 8701 CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC 8761 TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA 8821 GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT 8881 AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT 8941 CTGCTATGTG GCGCGGTATT ATCCCGTATT GACGCCGGGC AAGAGCAACT CGGTCGCCGC 9001 ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG 9061 GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG 9121 GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC 9181 ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA 9241 AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA 9301 ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT 9361 AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA 9421 TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG 9481 CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT 9541 AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT 9601 TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG 9661 AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA 9721 GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA 9781 ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA 9841 GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT 9901 GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA 9961 TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT 10021 ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG 10081 GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG 10141 CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA 10201 AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT 10261 CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG 10321 TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC 10381 TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC 10441 CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC 10501 GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG 10561 TGCGGTATTT CACACCGCAA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT 10621 TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC 10681 GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA 10741 AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG 10801 CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC 10861 CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT 10921 AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG 10981 GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG 11041 GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT 11101 ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC 11161 AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT 11221 GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT 11281 TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG 11341 TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA 11401 CGACAGGAGC ACGATCATGC GCACCCGTGG GGCCGCCATG CCGGCGATAA TGGCCTGCTT 11461 CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT 11521 TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC 11581 GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT 11641 CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA 11701 GGCTCTCAAG GGCATCGGTC GAGATCCCGG TGCCTAATGA GTGAGCTAAC TTACATTAAT 11761 TGCGTTGCGC TCACTGCCCG CTTTCCAGTC GGGAAACCTG TCGTGCCAGC TGCATTAATG 11821 AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCCAGGGTG GTTTTTCTTT 11881 TCACCAGTGA GACGGGCAAC AGCTGATTGC CCTTCACCGC CTGGCCCTGA GAGAGTTGCA 11941 GCAAGCGGTC CACGCTGGTT TGCCCCAGCA GGCGAAAATC CTGTTTGATG GTGGTTAACG 12001 GCGGGATATA ACATGAGCTG TCTTCGGTAT CGTCGTATCC CACTACCGAG ATATCCGCAC 12061 CAACGCGCAG CCCGGACTCG GTAATGGCGC GCATTGCGCC CAGCGCCATC TGATCGTTGG 12121 CAACCAGCAT CGCAGTGGGA ACGATGCCCT CATTCAGCAT TTGCATGGTT TGTTGAAAAC 12181 CGGACATGGC ACTCCAGTCG CCTTCCCGTT CCGCTATCGG CTGAATTTGA TTGCGAGTGA 12241 GATATTTATG CCAGCCAGCC AGACGCAGAC GCGCCGAGAC AGAACTTAAT GGGCCCGCTA 12301 ACAGCGCGAT TTGCTGGTGA CCCAATGCGA CCAGATGCTC CACGCCCAGT CGCGTACCGT 12361 CTTCATGGGA GAAAATAATA CTGTTGATGG GTGTCTGGTC AGAGACATCA AGAAATAACG 12421 CCGGAACATT AGTGCAGGCA GCTTCCACAG CAATGGCATC CTGGTCATCC AGCGGATAGT 12481 TAATGATCAG CCCACTGACG CGTTGCGCGA GAAGATTGTG CACCGCCGCT TTACAGGCTT 12541 CGACGCCGCT TCGTTCTACC ATCGACACCA CCACGCTGGC ACCCAGTTGA TCGGCGCGAG 12601 ATTTAATCGC CGCGACAATT TGCGACGGCG CGTGCAGGGC CAGACTGGAG GTGGCAACGC 12661 CAATCAGCAA CGACTGTTTG CCCGCCAGTT GTTGTGCCAC GCGGTTGGGA ATGTAATTCA 12721 GCTCCGCCAT CGCCGCTTCC ACTTTTTCCC GCGTTTTCGC AGAAACGTGG CTGGCCTGGT 12781 TCACCACGCG GGAAACGGTC TGATAAGAGA CACCGGCATA CTCTGCGACA TCGTATAACG 12841 TTACTGGTTT CACATTCACC ACCCTGAATT GACTCTCTTC CGGGCGCTAT CATGCCATAC 12901 CGCGAAAGGT TTTGCGCCAT TCGATGGTGT CCGGGATCTC GACGCTCTCC CTTATGCGAC 12961 TCCTGCATTA GGAAGCAGCC CAG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.