pCMV-PSMD2-3×FLAG-P2A-mCherry-

pCMV-PSMD2-3×FLAG-P2A-mCherry-Neo plasmid

PVT86150

pCMV-PSMD2-3×FLAG-P2A-mCherry-Neo plasmid Information

Plasmid name: pCMV-PSMD2(human)-3×FLAG-P2A-mCherry-Neo
Alias: NM_002808.5;P97; RPN1; S2; TRAP2
Gene lenght: 2724bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling: Red
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone: pCMV-MCS-P2A-mCherry-Neo
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-PSMD2-3×FLAG-P2A-mCherry-Neo plasmid Description

pCMV-PSMD2-3×FLAG-P2A-mCherry-Neo This plasmid is suitable for overexpression in mammalian cells. The CMV enhancer and CMV promoter carried by the plasmid can jointly initiate the efficient expression of the downstream human PSMD2 gene, promoting the N-terminal fusion expression of the PSMD2 gene with a 3×FLAG tag, which facilitates the detection, localization, and purification of the expressed fusion protein using FLAG antibodies. The C-terminal fusion with mCherry red fluorescent protein allows for intuitive observation of the expression location and level of the target gene. The plasmid carries a Neo/G418 resistance gene for selecting host cells that express the target gene.

pCMV-PSMD2-3×FLAG-P2A-mCherry-Neo plasmid Sequence

1 GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA 61 TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG 121 TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC 181 CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA 241 CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT 301 ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC 361 CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT 421 ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA 481 CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT 541 CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG 601 CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCGTTTAG TGAACCGTCA GATCGCCTGG 661 AGACGCCATC CACGCTGTTT TGACCTCCAT AGAAGACACC GGGACCGATC CAGCCTCCGG 721 ACTCTAGAGC TAGCGTTTAA ACTTAAGCTT GGTACCGAGC TCGGATCCGC CACCATGGAG 781 GAGGGAGGCC GGGACAAGGC GCCGGTGCAG CCCCAGCAGT CTCCAGCGGC GGCCCCCGGC 841 GGCACGGACG AGAAGCCGAG CGGCAAGGAG CGGCGGGATG CCGGGGACAA GGACAAAGAA 901 CAGGAGCTGT CTGAAGAGGA TAAACAGCTT CAAGATGAAC TGGAGATGCT CGTGGAACGA 961 CTAGGGGAGA AGGATACATC CCTGTATCGA CCAGCGCTGG AGGAATTGCG AAGGCAGATT 1021 CGTTCTTCTA CAACTTCCAT GACTTCAGTG CCCAAGCCTC TCAAATTTCT GCGTCCACAC 1081 TATGGCAAAC TGAAGGAAAT CTATGAGAAC ATGGCCCCTG GGGAGAATAA GCGTTTTGCT 1141 GCTGACATCA TCTCCGTTTT GGCCATGACC ATGAGTGGGG AGCGTGAGTG CCTCAAGTAT 1201 CGGCTAGTGG GCTCCCAGGA GGAATTGGCA TCATGGGGTC ATGAGTATGT CAGGCATCTG 1261 GCAGGAGAAG TGGCTAAGGA GTGGCAGGAG CTGGATGACG CAGAGAAGGT CCAGCGGGAG 1321 CCTCTGCTCA CTCTGGTGAA GGAAATCGTC CCCTATAACA TGGCCCACAA TGCAGAGCAT 1381 GAGGCTTGCG ACCTGCTTAT GGAAATTGAG CAGGTGGACA TGCTGGAGAA GGACATTGAT 1441 GAAAATGCAT ATGCAAAGGT CTGCCTTTAT CTCACCAGTT GTGTGAATTA CGTGCCTGAG 1501 CCTGAGAACT CAGCCCTACT GCGTTGTGCC CTGGGTGTGT TCCGAAAGTT TAGCCGCTTC 1561 CCTGAAGCTC TGAGATTGGC ATTGATGCTC AATGACATGG AGTTGGTAGA AGACATCTTC 1621 ACCTCCTGCA AGGATGTGGT AGTACAGAAA CAGATGGCAT TCATGCTAGG CCGGCATGGG 1681 GTGTTCCTGG AGCTGAGTGA AGATGTCGAG GAGTATGAGG ACCTGACAGA GATCATGTCC 1741 AATGTACAGC TCAACAGCAA CTTCTTGGCC TTAGCTCGGG AGCTGGACAT CATGGAGCCC 1801 AAGGTGCCTG ATGACATCTA CAAAACCCAC CTAGAGAACA ACAGGTTTGG GGGCAGTGGC 1861 TCTCAGGTGG ACTCTGCCCG CATGAACCTG GCCTCCTCTT TTGTGAATGG CTTTGTGAAT 1921 GCAGCTTTTG GCCAAGACAA GCTGCTAACA GATGATGGCA ACAAATGGCT TTACAAGAAC 1981 AAGGACCACG GAATGTTGAG TGCAGCTGCA TCTCTTGGGA TGATTCTGCT GTGGGATGTG 2041 GATGGTGGCC TCACCCAGAT TGACAAGTAC CTGTACTCCT CTGAGGACTA CATTAAGTCA 2101 GGAGCTCTTC TTGCCTGTGG CATAGTGAAC TCTGGGGTCC GGAATGAGTG TGACCCTGCT 2161 CTGGCACTGC TCTCAGACTA TGTTCTCCAC AACAGCAACA CCATGAGACT TGGTTCCATC 2221 TTTGGGCTAG GCTTGGCTTA TGCTGGCTCA AATCGTGAAG ATGTCCTAAC ACTGCTGCTG 2281 CCTGTGATGG GAGATTCAAA GTCCAGCATG GAGGTGGCAG GTGTCACAGC TTTAGCCTGT 2341 GGAATGATAG CAGTAGGGTC CTGCAATGGA GATGTAACTT CCACTATCCT TCAGACCATC 2401 ATGGAGAAGT CAGAGACTGA GCTCAAGGAT ACTTATGCTC GTTGGCTTCC TCTTGGACTG 2461 GGTCTCAACC ACCTGGGGAA GGGTGAGGCC ATCGAGGCAA TCCTGGCTGC ACTGGAGGTT 2521 GTGTCAGAGC CATTCCGCAG TTTTGCCAAC ACACTGGTGG ATGTGTGTGC ATATGCAGGC 2581 TCTGGGAATG TGCTGAAGGT GCAGCAGCTG CTCCACATTT GTAGCGAACA CTTTGACTCC 2641 AAAGAGAAGG AGGAAGACAA AGACAAGAAG GAAAAGAAAG ACAAGGACAA GAAGGAAGCC 2701 CCTGCTGACA TGGGAGCACA TCAGGGAGTG GCTGTTCTGG GGATTGCCCT TATTGCTATG 2761 GGGGAGGAGA TTGGTGCAGA GATGGCATTA CGAACCTTTG GCCACTTGCT GAGATATGGG 2821 GAGCCTACAC TCCGGAGGGC TGTACCTTTA GCACTGGCCC TCATCTCTGT TTCAAATCCA 2881 CGACTCAACA TCCTGGATAC CCTAAGCAAA TTCTCTCATG ATGCTGATCC AGAAGTTTCC 2941 TATAACTCCA TTTTTGCCAT GGGCATGGTG GGCAGTGGTA CCAATAATGC CCGTCTGGCT 3001 GCAATGCTGC GCCAGTTAGC TCAATATCAT GCCAAGGACC CAAACAACCT CTTCATGGTG 3061 CGCTTGGCAC AGGGCCTGAC ACATTTAGGG AAGGGCACCC TTACCCTCTG CCCCTACCAC 3121 AGCGACCGGC AGCTTATGAG CCAGGTGGCC GTGGCTGGAC TGCTCACTGT GCTTGTCTCT 3181 TTCCTGGATG TTCGAAACAT TATTCTAGGC AAATCACACT ATGTATTGTA TGGGCTGGTG 3241 GCTGCCATGC AGCCCCGAAT GCTGGTTACG TTTGATGAGG AGCTGCGGCC ATTGCCAGTG 3301 TCTGTCCGTG TGGGCCAGGC AGTGGATGTG GTGGGCCAGG CTGGCAAGCC GAAGACTATC 3361 ACAGGGTTCC AGACGCATAC AACCCCAGTG TTGTTGGCCC ACGGGGAACG GGCAGAATTG 3421 GCCACTGAGG AGTTTCTTCC TGTTACCCCC ATTCTGGAAG GTTTTGTTAT CCTTCGGAAG 3481 AACCCCAATT ATGATCTCGA CTACAAAGAC CATGACGGTG ATTATAAAGA TCATGACATC 3541 GACTACAAGG ATGACGATGA CAAGGAATTC GGAAGCGGAG CTACTAACTT CAGCCTGCTG 3601 AAGCAGGCTG GCGACGTGGA GGAGAACCCT GGACCTATGG TGAGCAAGGG CGAGGAGGAT 3661 AACATGGCCA TCATCAAGGA GTTCATGCGC TTCAAGGTGC ACATGGAGGG CTCCGTGAAC 3721 GGCCACGAGT TCGAGATCGA GGGCGAGGGC GAGGGCCGCC CCTACGAGGG CACCCAGACC 3781 GCCAAGCTGA AGGTGACCAA GGGTGGCCCC CTGCCCTTCG CCTGGGACAT CCTGTCCCCT 3841 CAGTTCATGT ACGGCTCCAA GGCCTACGTG AAGCACCCCG CCGACATCCC CGACTACTTG 3901 AAGCTGTCCT TCCCCGAGGG CTTCAAGTGG GAGCGCGTGA TGAACTTCGA GGACGGCGGC 3961 GTGGTGACCG TGACCCAGGA CTCCTCCCTG CAGGACGGCG AGTTCATCTA CAAGGTGAAG 4021 CTGCGCGGCA CCAACTTCCC CTCCGACGGC CCCGTAATGC AGAAGAAGAC CATGGGCTGG 4081 GAGGCCTCCT CCGAGCGGAT GTACCCCGAG GACGGCGCCC TGAAGGGCGA GATCAAGCAG 4141 AGGCTGAAGC TGAAGGACGG CGGCCACTAC GACGCTGAGG TCAAGACCAC CTACAAGGCC 4201 AAGAAGCCCG TGCAGCTGCC CGGCGCCTAC AACGTCAACA TCAAGTTGGA CATCACCTCC 4261 CACAACGAGG ACTACACCAT CGTGGAACAG TACGAACGCG CCGAGGGCCG CCACTCCACC 4321 GGCGGCATGG ACGAGCTGTA CAAGTAACTC GAGTCTAGAG GGCCCCTTAA GGGTTCGATC 4381 CCTACCGGTT AGTAATGAGT TTGATATCTC GACAATCAAC CTCTGGATTA CAAAATTTGT 4441 GAAAGATTGA CTGGTATTCT TAACTATGTT GCTCCTTTTA CGCTATGTGG ATACGCTGCT 4501 TTAATGCCTT TGTATCATGC TATTGCTTCC CGTATGGCTT TCATTTTCTC CTCCTTGTAT 4561 AAATCCTGGT TGCTGTCTCT TTATGAGGAG TTGTGGCCCG TTGTCAGGCA ACGTGGCGTG 4621 GTGTGCACTG TGTTTGCTGA CGCAACCCCC ACTGGTTGGG GCATTGCCAC CACCTGTCAG 4681 CTCCTTTCCG GGACTTTCGC TTTCCCCCTC CCTATTGCCA CGGCGGAACT CATCGCCGCC 4741 TGCCTTGCCC GCTGCTGGAC AGGGGCTCGG CTGTTGGGCA CTGACAATTC CGTGGTGTTG 4801 TCGGGGAAGC TGACGTCCTT TCCATGGCTG CTCGCCTGTG TTGCCACCTG GATTCTGCGC 4861 GGGACGTCCT TCTGCTACGT CCCTTCGGCC CTCAATCCAG CGGACCTTCC TTCCCGCGGC 4921 CTGCTGCCGG CTCTGCGGCC TCTTCCGCGT CTTCGCCTTC GCCCTCAGAC GAGTCGGATC 4981 TCCCTTTGGG CCGCCTCCCC GCCTGGAAAC GGGGGAGGCT AACTGAAACA CGGAAGGAGA 5041 CAATACCGGA AGGAACCCGC GCTATGACGG CAATAAAAAG ACAGAATAAA ACGCACGGGT 5101 GTTGGGTCGT TTGTTCATAA ACGCGGGGTT CGGTCCCAGG GCTGGCACTC TGTCGATACC 5161 CCACCGAGAC CCCATTGGGG CCAATACGCC CGCGTTTCTT CCTTTTCCCC ACCCCACCCC 5221 CCAAGTTCGG GTGAAGGCCC AGGGCTCGCA GCCAACGTCG GGGCGGCAGG CCCTGCCATA 5281 GCAGATCTGC GCAGCTGGGG CTCTAGGGGG TATCCCCACG CGCCCTGTAG CGGCGCATTA 5341 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 5401 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 5461 GCTCTAAATC GGGGCATCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 5521 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 5581 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 5641 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGGG GATTTCGGCC 5701 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTAATT CTGTGGAATG 5761 TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA 5821 TGCATCTCAA TTAGTCAGCA ACCAGGTGTG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA 5881 GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCATAGT CCCGCCCCTA ACTCCGCCCA 5941 TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC CCATGGCTGA CTAATTTTTT 6001 TTATTTATGC AGAGGCCGAG GCCGCCTCTG CCTCTGAGCT ATTCCAGAAG TAGTGAGGAG 6061 GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA AGCTCCCGGG AGCTTGTATA TCCATTTTCG 6121 GATCTGATCA AGAGACAGGA TGAGGATCGT TTCGCATGAT TGAACAAGAT GGATTGCACG 6181 CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC TATTCGGCTA TGACTGGGCA CAACAGACAA 6241 TCGGCTGCTC TGATGCCGCC GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG 6301 TCAAGACCGA CCTGTCCGGT GCCCTGAATG AACTGCAGGA CGAGGCAGCG CGGCTATCGT 6361 GGCTGGCCAC GACGGGCGTT CCTTGCGCAG CTGTGCTCGA CGTTGTCACT GAAGCGGGAA 6421 GGGACTGGCT GCTATTGGGC GAAGTGCCGG GGCAGGATCT CCTGTCATCT CACCTTGCTC 6481 CTGCCGAGAA AGTATCCATC ATGGCTGATG CAATGCGGCG GCTGCATACG CTTGATCCGG 6541 CTACCTGCCC ATTCGACCAC CAAGCGAAAC ATCGCATCGA GCGAGCACGT ACTCGGATGG 6601 AAGCCGGTCT TGTCGATCAG GATGATCTGG ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG 6661 AACTGTTCGC CAGGCTCAAG GCGCGCATGC CCGACGGCGA GGATCTCGTC GTGACCCATG 6721 GCGATGCCTG CTTGCCGAAT ATCATGGTGG AAAATGGCCG CTTTTCTGGA TTCATCGACT 6781 GTGGCCGGCT GGGTGTGGCG GACCGCTATC AGGACATAGC GTTGGCTACC CGTGATATTG 6841 CTGAAGAGCT TGGCGGCGAA TGGGCTGACC GCTTCCTCGT GCTTTACGGT ATCGCCGCTC 6901 CCGATTCGCA GCGCATCGCC TTCTATCGCC TTCTTGACGA GTTCTTCTGA GCGGGACTCT 6961 GGGGTTCGCG AAATGACCGA CCAAGCGACG CCCAACCTGC CATCACGAGA TTTCGATTCC 7021 ACCGCCGCCT TCTATGAAAG GTTGGGCTTC GGAATCGTTT TCCGGGACGC CGGCTGGATG 7081 ATCCTCCAGC GCGGGGATCT CATGCTGGAG TTCTTCGCCC ACCCCAACTT GTTTATTGCA 7141 GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT TCACAAATAA AGCATTTTTT 7201 TCACTGCATT CTAGTTGTGG TTTGTCCAAA CTCATCAATG TATCTTATCA TGTCTGTATA 7261 CCGTCGACCT CTAGCTAGAG CTTGGCGTAA TCATGGTCAT AGCTGTTTCC TGTGTGAAAT 7321 TGTTATCCGC TCACAATTCC ACACAACATA CGAGCCGGAA GCATAAAGTG TAAAGCCTGG 7381 GGTGCCTAAT GAGTGAGCTA ACTCACATTA ATTGCGTTGC GCTCACTGCC CGCTTTCCAG 7441 TCGGGAAACC TGTCGTGCCA GCTGCATTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT 7501 TTGCGTATTG GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG 7561 CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG 7621 GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG 7681 GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA 7741 CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT 7801 GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC 7861 TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CAATGCTCAC GCTGTAGGTA TCTCAGTTCG 7921 GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC 7981 TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA 8041 CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG 8101 TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGGA CAGTATTTGG TATCTGCGCT 8161 CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC 8221 ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA 8281 TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA 8341 CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT 8401 TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC 8461 CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT 8521 GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT 8581 GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG 8641 CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT 8701 ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT 8761 GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC 8821 TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT 8881 AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG 8941 GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG 9001 ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT 9061 TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC 9121 ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT 9181 TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT 9241 TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG 9301 AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 9361 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 9421 CGCACATTTC CCCGAAAAGT GCCACCTGAC GTCGACGGAT CGGGAGATCT CCCGATCCCC 9481 TATGGTCGAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATCTGCTCC 9541 CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT GCGCGAGCAA AATTTAAGCT ACAACAAGGC 9601 AAGGCTTGAC CGACAATTGC ATGAAGAATC TGCTTAGG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.