pMMLV-ER-mRuby3-KDEL-BSD plasm

pMMLV-ER-mRuby3-KDEL-BSD plasmid

PVT86112

pMMLV-ER-mRuby3-KDEL-BSD plasmid Information

Plasmid name: pMMLV-ER-mRuby3-KDEL-BSD
Alias:
Gene lenght:
Host: Mammalian cells,Retrovirus
Use(s): Protein Expression,autophagy
Fragment type: ORF
Species: Empty Vector
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Red
Promoter: MMLV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 30°C
Vector backbone: pMMLV-mRuby3-LC3B(rat)-BSD
Forward primer: pBABE-5
Reverse primer:
Copy number: High
Induction:

pMMLV-ER-mRuby3-KDEL-BSD plasmid Description

pMMLV-ER-mRuby3-KDEL-BSD This is a retroviral vector for gene expression in mammalian cells. The MMLV promoter carried by the plasmid can initiate the transcription of downstream human genes. The plasmid carries the mRuby3 red fluorescent protein, making it easy to visually detect the expression and localization of the target gene. The ER localization signal and KDEL sequence in the plasmid help localize the expressed protein to the endoplasmic reticulum (ER) and maintain protein stability in the ER through the KDEL-mediated recycling pathway. Furthermore, the plasmid carries LTR elements for retroviral packaging, facilitating the stable integration of the gene into host cells. The plasmid also carries a blast resistance gene for screening host cells expressing the target gene.

pMMLV-ER-mRuby3-KDEL-BSD plasmid Sequence

1 AATGAAAGAC CCCACCTGTA GGTTTGGCAA GCTAGCTTAA GTAACGCCAT TTTGCAAGGC 61 ATGGAAAAAT ACATAACTGA GAATAGAGAA GTTCAGATCA AGGTCAGGAA CAGATGGAAC 121 AGCTGAATAT GGGCCAAACA GGATATCTGT GGTAAGCAGT TCCTGCCCCG GCTCAGGGCC 181 AAGAACAGAT GGAACAGCTG AATATGGGCC AAACAGGATA TCTGTGGTAA GCAGTTCCTG 241 CCCCGGCTCA GGGCCAAGAA CAGATGGTCC CCAGATGCGG TCCAGCCCTC AGCAGTTTCT 301 AGAGAACCAT CAGATGTTTC CAGGGTGCCC CAAGGACCTG AAATGACCCT GTGCCTTATT 361 TGAACTAACC AATCAGTTCG CTTCTCGCTT CTGTTCGCGC GCTTCTGCTC CCCGAGCTCA 421 ATAAAAGAGC CCACAACCCC TCACTCGGCG CGCCAGTCCT CCGATTGACT GAGTCGCCCG 481 GGTACCCGTG TATCCAATAA ACCCTCTTGC AGTTGCATCC GACTTGTGGT CTCGCTGTTC 541 CTTGGGAGGG TCTCCTCTGA GTGATTGACT ACCCGTCAGC GGGGGTCTTT CATTTGGGGG 601 CTCGTCCGGG ATCGGGAGAC CCCTGCCCAG GGACCACCGA CCCACCACCG GGAGGTAAGC 661 TGGCCAGCAA CTTATCTGTG TCTGTCCGAT TGTCTAGTGT CTATGACTGA TTTTATGCGC 721 CTGCGTCGGT ACTAGTTAGC TAACTAGCTC TGTATCTGGC GGACCCGTGG TGGAACTGAC 781 GAGTTCGGAA CACCCGGCCG CAACCCTGGG AGACGTCCCA GGGACTTCGG GGGCCGTTTT 841 TGTGGCCCGA CCTGAGTCCA AAAATCCCGA TCGTTTTGGA CTCTTTGGTG CACCCCCCTT 901 AGAGGAGGGA TATGTGGTTC TGGTAGGAGA CGAGAACCTA AAACAGTTCC CGCCTCCGTC 961 TGAATTTTTG CTTTCGGTTT GGGACCGAAG CCGCGCCGCG CGTCTTGTCT GCTGCAGCAT 1021 CGTTCTGTGT TGTCTCTGTC TGACTGTGTT TCTGTATTTG TCTGAAAATA TGGGCCCGGG 1081 CCAGACTGTT ACCACTCCCT TAAGTTTGAC CTTAGGTCAC TGGAAAGATG TCGAGCGGAT 1141 CGCTCACAAC CAGTCGGTAG ATGTCAAGAA GAGACGTTGG GTTACCTTCT GCTCTGCAGA 1201 ATGGCCAACC TTTAACGTCG GATGGCCGCG AGACGGCACC TTTAACCGAG ACCTCATCAC 1261 CCAGGTTAAG ATCAAGGTCT TTTCACCTGG CCCGCATGGA CACCCAGACC AGGTCCCCTA 1321 CATCGTGACC TGGGAAGCCT TGGCTTTTGA CCCCCCTCCC TGGGTCAAGC CCTTTGTACA 1381 CCCTAAGCCT CCGCCTCCTC TTCCTCCATC CGCCCCGTCT CTCCCCCTTG AACCTCCTCG 1441 TTCGACCCCG CCTCGATCCT CCCTTTATCC AGCCCTCACT CCTTCTCTAG GCGCCCCCAT 1501 ATGGCCATAT GAGATCTTAT ATGGGGCACC CCCGCCCCTT GTAAACTTCC CTGACCCTGA 1561 CATGACAAGA GTTACTAACA GCCCCTCTCT CCAAGCTCAC TTACAGGCTC TCTACTTAGT 1621 CCAGCACGAA GTCTGGAGAC CTCTGGCGGC AGCCTACCAA GAACAACTGG ACCGACCGGT 1681 GGTACCTCAC CCTTACCGAG TCGGCGACAC AGTGTGGGTC CGCCGACACC AGACTAAGAA 1741 CCTAGAACCT CGCTGGAAAG GACCTTACAC AGTCCTGCTG ACCACCCCCA CCGCCCTCAA 1801 AGTAGACGGC ATCGCAGCTT GGATACACGC CGCCCACGTG AAGGCTGCCG ACCCCGGGGG 1861 TGGACCATCC TCTAGACTGC CGGATCTCCC GCCACCATGC TGCTATCCGT GCCGTTGCTG 1921 CTCGGCCTCC TCGGCCTGGC CGTCGCCGAC CGGTCGCACA CCATGGTGTC TAAGGGCGAA 1981 GAGCTGATCA AGGAAAATAT GCGTATGAAG GTGGTCATGG AAGGTTCGGT CAACGGCCAC 2041 CAATTCAAAT GCACAGGTGA AGGAGAAGGC AGGCCGTACG AGGGAGTGCA AACCATGAGG 2101 ATCAAAGTCA TCGAGGGAGG ACCCCTGCCA TTTGCCTTTG ACATTCTTGC CACGTCGTTC 2161 ATGTATGGCA GCCGTACTTT TATCAAGTAC CCGGCAGACA TTCCTGATTT CTTTAAACAG 2221 TCCTTTCCTG AGGGTTTTAC TTGGGAAAGA GTTACGAGAT ACGAAGATGG TGGAGTCGTC 2281 ACCGTCACGC AGGACACCAG CCTTGAGGAT GGCGAACTCG TTTACAACGT CAAAGTCAGA 2341 GGGGTAAACT TTCCCTCCAA TGGTCCCGTG ATGCAGAAGA AGACCAAGGG TTGGGAGCCT 2401 AATACAGAGA TGATGTATCC AGCAGATGGT GGTCTGAGGG GATACACTGA TATTGCACTG 2461 AAAGTTGATG GTGGTGGCCA TCTGCATTGC AATTTCGTAA CAACTTACAG GTCAAAAAAG 2521 ACCGTCGGGA ACATCAAGAT GCCCGGTGTC CATGCCGTTG ATCACCGCCT GGAAAGGATA 2581 GAGGAAAGTG ACAATGAAAC GTACGTAGTA CAACGCGAAG TCGCAGTTGC CAAGTACTCC 2641 AATCTTGGTG GTGGTATGGA TGAACTTTAT AAGAGATCGT ACAAGAAGGA CGAGCTGTGA 2701 GGATCCCAGT GTGGTGGTAC GGGAATTCCT GCAGGCCTCG AGGGCCGGCG CGCCGCGGCC 2761 GCTACGTAAA TTCCGCCCCC CCCCCCCCTC CCCCCCCCCT AACGTTACTG GCCGAAGCCG 2821 CTTGGAATAA GGCCGGTGTG CGTTTGTCTA TATGTTATTT TCCACCATAT TGCCGTCTTT 2881 TGGCAATGTG AGGGCCCGGA AACCTGGCCC TGTCTTCTTG ACGAGCATTC CTAGGGGTCT 2941 TTCCCCTCTC GCCAAAGGAA TGCAAGGTCT GTTGAATGTC GTGAAGGAAG CAGTTCCTCT 3001 GGAAGCTTCT TGAAGACAAA CAACGTCTGT AGCGACCCTT TGCAGGCAGC GGAACCCCCC 3061 ACCTGGCGAC AGGTGCCTCT GCGGCCAAAA GCCACGTGTA TAAGATACAC CTGCAAAGGC 3121 GGCACAACCC CAGTGCCACG TTGTGAGTTG GATAGTTGTG GAAAGAGTCA AATGGCTCTC 3181 CTCAAGCGTA TTCAACAAGG GGCTGAAGGA TGCCCAGAAG GTACCCCATT GTATGGGATC 3241 TGATCTGGGG CCTCGGTGCA CATGCTTTAC ATGTGTTTAG TCGAGGTTAA AAAAACGTCT 3301 AGGCCCCCCG AACCACGGGG ACGTGGTTTT CCTTTGAAAA ACACGATGAT AATATGGCCA 3361 CAACCATGGC CAAGCCTTTG TCTCAAGAAG AATCCACCCT CATTGAAAGA GCAACGGCTA 3421 CAATCAACAG CATCCCCATC TCTGAAGACT ACAGCGTCGC CAGCGCAGCT CTCTCTAGCG 3481 ACGGCCGCAT CTTCACTGGT GTCAATGTAT ATCATTTTAC TGGGGGACCT TGTGCAGAAC 3541 TCGTGGTGCT GGGCACTGCT GCTGCTGCGG CAGCTGGCAA CCTGACTTGT ATCGTCGCGA 3601 TCGGAAATGA GAACAGGGGC ATCTTGAGCC CCTGCGGACG GTGCCGACAG GTGCTTCTCG 3661 ATCTGCATCC TGGGATCAAA GCCATAGTGA AGGACAGTGA TGGACAGCCG ACGGCAGTTG 3721 GGATTCGTGA ATTGCTGCCC TCTGGTTATG TGTGGGAGGG CTAACGCCCG CCCCACGACC 3781 CGCAGCGCCC GACCGAAAGG AGCGCACGAC CCCATGGCTC GACGATAAAA TAAAAGATTT 3841 TATTTAGTCT CCAGAAAAAG GGGGGAATGA AAGACCCCAC CTGTAGGTTT GGCAAGCTAG 3901 CTTAAGTAAC GCCATTTTGC AAGGCATGGA AAAATACATA ACTGAGAATA GAGAAGTTCA 3961 GATCAAGGTC AGGAACAGAT GGAACAGCTG AATATGGGCC AAACAGGATA TCTGTGGTAA 4021 GCAGTTCCTG CCCCGGCTCA GGGCCAAGAA CAGATGGAAC AGCTGAATAT GGGCCAAACA 4081 GGATATCTGT GGTAAGCAGT TCCTGCCCCG GCTCAGGGCC AAGAACAGAT GGTCCCCAGA 4141 TGCGGTCCAG CCCTCAGCAG TTTCTAGAGA ACCATCAGAT GTTTCCAGGG TGCCCCAAGG 4201 ACCTGAAATG ACCCTGTGCC TTATTTGAAC TAACCAATCA GTTCGCTTCT CGCTTCTGTT 4261 CGCGCGCTTC TGCTCCCCGA GCTCAATAAA AGAGCCCACA ACCCCTCACT CGGGGCGCCA 4321 GTCCTCCGAT TGACTGAGTC GCCCGGGTAC CCGTGTATCC AATAAACCCT CTTGCAGTTG 4381 CATCCGACTT GTGGTCTCGC TGTTCCTTGG GAGGGTCTCC TCTGAGTGAT TGACTACCCG 4441 TCAGCGGGGG TCTTTCACAT GCAGCATGTA TCAAAATTAA TTTGGTTTTT TTTCTTAAGT 4501 ATTTACATTA AATGGCCATA GTTGCATTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT 4561 TTGCGTATTG GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG 4621 CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG 4681 GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG 4741 GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA 4801 CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT 4861 GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC 4921 TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG 4981 GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC 5041 TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA 5101 CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG 5161 TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT 5221 CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC 5281 ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA 5341 TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA 5401 CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTGCGG 5461 CCGGCCGCAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT 5521 TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC 5581 TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA 5641 TGATACCGCG AGACCCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG 5701 GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT 5761 GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA 5821 TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT 5881 CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT 5941 TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG 6001 CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG 6061 AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG 6121 CGTCAACACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA 6181 AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT 6241 AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT 6301 GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT 6361 GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA 6421 TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT 6481 TTCCCCGAAA AGTGCCACCT GACGTCTAAG AAACCATTAT TATCATGACA TTAACCTATA 6541 AAAATAGGCG TATCACGAGG CCCTTTCGTC TTCACTCGAG GAGCTTGGCC CATTGCATAC 6601 GTTGTATCCA TATCATAATA TGTACATTTA TATTGGCTCA TGTCCAACAT TACCGCCATG 6661 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 6721 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 6781 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 6841 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 6901 TCAAGTGTAT CATATGCCAA GTACGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 6961 CTGGCATTAT GCCCAGTACA TGACCTTATG GGACTTTCCT ACTTGGCAGT ACATCTACGT 7021 ATTAGTCATC GCTATTACCA TGCTAGAGAA CCATCAGATG TTTCCAGGGT GCCCCAAGGA 7081 CCTGAAATGA CCCTGTGCCT TATTTGAACT AACCAATCAG TTCGCTTCTC GCTTCTGTTC 7141 GCGCGCTTCT GCTCCCCGAG CTCAATAAAA GAGCCCACAA CCCCTCACTC GGCGCGCCAG 7201 TCCTCCGATT GACTGAGTCG CCCGGGTACC CGTGTATCCA ATAAACCCTC TTGCAGTTGC 7261 ATCCGACTTG TGG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.