pMMLV-PRKN-3×FLAG-BSD plasmid

pMMLV-PRKN-3×FLAG-BSD plasmid

PVT86105

pMMLV-PRKN-3×FLAG-BSD plasmid Information

Plasmid name: pMMLV-PRKN(human)-3×FLAG-BSD
Alias: NM_004562.3;AR-JP; LPRS2; PARK2; PDJ
Gene lenght: 1395bp
Host: Mammalian cells,Retrovirus
Use(s): Protein Expression,autophagy
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Blast
Fluorescent labeling:
Promoter: MMLV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone: pMMLV-mRuby3-LC3B(rat)-BSD
Forward primer: pBABE-5
Reverse primer:
Copy number: High
Induction:

pMMLV-PRKN-3×FLAG-BSD plasmid Description

pMMLV-PRKN-3×FLAG-BSD This is a low-copy plasmid suitable for overexpression in mammalian cells. The plasmid contains an MMLV promoter that can initiate the transcription of the human PRKN gene, promoting the C-terminal fusion expression of the PRKN gene with a 3×FLAG tag, facilitating detection, localization, and purification of the expressed fusion protein using FLAG antibodies. Additionally, the plasmid carries LTR elements for retroviral packaging, facilitating stable integration of the gene into host cells. The plasmid carries a blast resistance gene for selecting host cells that express the target gene.

pMMLV-PRKN-3×FLAG-BSD plasmid Sequence

1 AATGAAAGAC CCCACCTGTA GGTTTGGCAA GCTAGCTTAA GTAACGCCAT TTTGCAAGGC 61 ATGGAAAAAT ACATAACTGA GAATAGAGAA GTTCAGATCA AGGTCAGGAA CAGATGGAAC 121 AGCTGAATAT GGGCCAAACA GGATATCTGT GGTAAGCAGT TCCTGCCCCG GCTCAGGGCC 181 AAGAACAGAT GGAACAGCTG AATATGGGCC AAACAGGATA TCTGTGGTAA GCAGTTCCTG 241 CCCCGGCTCA GGGCCAAGAA CAGATGGTCC CCAGATGCGG TCCAGCCCTC AGCAGTTTCT 301 AGAGAACCAT CAGATGTTTC CAGGGTGCCC CAAGGACCTG AAATGACCCT GTGCCTTATT 361 TGAACTAACC AATCAGTTCG CTTCTCGCTT CTGTTCGCGC GCTTCTGCTC CCCGAGCTCA 421 ATAAAAGAGC CCACAACCCC TCACTCGGCG CGCCAGTCCT CCGATTGACT GAGTCGCCCG 481 GGTACCCGTG TATCCAATAA ACCCTCTTGC AGTTGCATCC GACTTGTGGT CTCGCTGTTC 541 CTTGGGAGGG TCTCCTCTGA GTGATTGACT ACCCGTCAGC GGGGGTCTTT CATTTGGGGG 601 CTCGTCCGGG ATCGGGAGAC CCCTGCCCAG GGACCACCGA CCCACCACCG GGAGGTAAGC 661 TGGCCAGCAA CTTATCTGTG TCTGTCCGAT TGTCTAGTGT CTATGACTGA TTTTATGCGC 721 CTGCGTCGGT ACTAGTTAGC TAACTAGCTC TGTATCTGGC GGACCCGTGG TGGAACTGAC 781 GAGTTCGGAA CACCCGGCCG CAACCCTGGG AGACGTCCCA GGGACTTCGG GGGCCGTTTT 841 TGTGGCCCGA CCTGAGTCCA AAAATCCCGA TCGTTTTGGA CTCTTTGGTG CACCCCCCTT 901 AGAGGAGGGA TATGTGGTTC TGGTAGGAGA CGAGAACCTA AAACAGTTCC CGCCTCCGTC 961 TGAATTTTTG CTTTCGGTTT GGGACCGAAG CCGCGCCGCG CGTCTTGTCT GCTGCAGCAT 1021 CGTTCTGTGT TGTCTCTGTC TGACTGTGTT TCTGTATTTG TCTGAAAATA TGGGCCCGGG 1081 CCAGACTGTT ACCACTCCCT TAAGTTTGAC CTTAGGTCAC TGGAAAGATG TCGAGCGGAT 1141 CGCTCACAAC CAGTCGGTAG ATGTCAAGAA GAGACGTTGG GTTACCTTCT GCTCTGCAGA 1201 ATGGCCAACC TTTAACGTCG GATGGCCGCG AGACGGCACC TTTAACCGAG ACCTCATCAC 1261 CCAGGTTAAG ATCAAGGTCT TTTCACCTGG CCCGCATGGA CACCCAGACC AGGTCCCCTA 1321 CATCGTGACC TGGGAAGCCT TGGCTTTTGA CCCCCCTCCC TGGGTCAAGC CCTTTGTACA 1381 CCCTAAGCCT CCGCCTCCTC TTCCTCCATC CGCCCCGTCT CTCCCCCTTG AACCTCCTCG 1441 TTCGACCCCG CCTCGATCCT CCCTTTATCC AGCCCTCACT CCTTCTCTAG GCGCCCCCAT 1501 ATGGCCATAT GAGATCTTAT ATGGGGCACC CCCGCCCCTT GTAAACTTCC CTGACCCTGA 1561 CATGACAAGA GTTACTAACA GCCCCTCTCT CCAAGCTCAC TTACAGGCTC TCTACTTAGT 1621 CCAGCACGAA GTCTGGAGAC CTCTGGCGGC AGCCTACCAA GAACAACTGG ACCGACCGGT 1681 GGTACCTCAC CCTTACCGAG TCGGCGACAC AGTGTGGGTC CGCCGACACC AGACTAAGAA 1741 CCTAGAACCT CGCTGGAAAG GACCTTACAC AGTCCTGCTG ACCACCCCCA CCGCCCTCAA 1801 AGTAGACGGC ATCGCAGCTT GGATACACGC CGCCCACGTG AAGGCTGCCG ACCCCGGGGG 1861 TGGACCATCC TCTAGACTGC CGGATCTCCC GCCACCATGA TAGTGTTTGT CAGGTTCAAC 1921 TCCAGCCATG GTTTCCCAGT GGAGGTCGAT TCTGACACCA GCATCTTCCA GCTCAAGGAG 1981 GTGGTTGCTA AGCGACAGGG GGTTCCGGCT GACCAGTTGC GTGTGATTTT CGCAGGGAAG 2041 GAGCTGAGGA ATGACTGGAC TGTGCAGAAT TGTGACCTGG ATCAGCAGAG CATTGTTCAC 2101 ATTGTGCAGA GACCGTGGAG AAAAGGTCAA GAAATGAATG CAACTGGAGG CGACGACCCC 2161 AGAAACGCGG CGGGAGGCTG TGAGCGGGAG CCCCAGAGCT TGACTCGGGT GGACCTCAGC 2221 AGCTCAGTCC TCCCAGGAGA CTCTGTGGGG CTGGCTGTCA TTCTGCACAC TGACAGCAGG 2281 AAGGACTCAC CACCAGCTGG AAGTCCAGCA GGTAGATCAA TCTACAACAG CTTTTATGTG 2341 TATTGCAAAG GCCCCTGTCA AAGAGTGCAG CCGGGAAAAC TCAGGGTACA GTGCAGCACC 2401 TGCAGGCAGG CAACGCTCAC CTTGACCCAG GGTCCATCTT GCTGGGATGA TGTTTTAATT 2461 CCAAACCGGA TGAGTGGTGA ATGCCAATCC CCACACTGCC CTGGGACTAG TGCAGAATTT 2521 TTCTTTAAAT GTGGAGCACA CCCCACCTCT GACAAGGAAA CATCAGTAGC TTTGCACCTG 2581 ATCGCAACAA ATAGTCGGAA CATCACTTGC ATTACGTGCA CAGACGTCAG GAGCCCCGTC 2641 CTGGTTTTCC AGTGCAACTC CCGCCACGTG ATTTGCTTAG ACTGTTTCCA CTTATACTGT 2701 GTGACAAGAC TCAATGATCG GCAGTTTGTT CACGACCCTC AACTTGGCTA CTCCCTGCCT 2761 TGTGTGGCTG GCTGTCCCAA CTCCTTGATT AAAGAGCTCC ATCACTTCAG GATTCTGGGA 2821 GAAGAGCAGT ACAACCGGTA CCAGCAGTAT GGTGCAGAGG AGTGTGTCCT GCAGATGGGG 2881 GGCGTGTTAT GCCCCCGCCC TGGCTGTGGA GCGGGGCTGC TGCCGGAGCC TGACCAGAGG 2941 AAAGTCACCT GCGAAGGGGG CAATGGCCTG GGCTGTGGGT TTGCCTTCTG CCGGGAATGT 3001 AAAGAAGCGT ACCATGAAGG GGAGTGCAGT GCCGTATTTG AAGCCTCAGG AACAACTACT 3061 CAGGCCTACA GAGTCGATGA AAGAGCCGCC GAGCAGGCTC GTTGGGAAGC AGCCTCCAAA 3121 GAAACCATCA AGAAAACCAC CAAGCCCTGT CCCCGCTGCC ATGTACCAGT GGAAAAAAAT 3181 GGAGGCTGCA TGCACATGAA GTGTCCGCAG CCCCAGTGCA GGCTCGAGTG GTGCTGGAAC 3241 TGTGGCTGCG AGTGGAACCG CGTCTGCATG GGGGACCACT GGTTCGACGT GCTCGAGGAC 3301 TACAAAGACC ATGACGGTGA TTATAAAGAT CATGACATCG ACTACAAGGA TGACGATGAC 3361 AAGTAGGATC CCAGTGTGGT GGTACGGGAA TTCCTGCAGG CCTCGAGGGC CGGCGCGCCG 3421 CGGCCGCTAC GTAAATTCCG CCCCCCCCCC CCCTCCCCCC CCCCTAACGT TACTGGCCGA 3481 AGCCGCTTGG AATAAGGCCG GTGTGCGTTT GTCTATATGT TATTTTCCAC CATATTGCCG 3541 TCTTTTGGCA ATGTGAGGGC CCGGAAACCT GGCCCTGTCT TCTTGACGAG CATTCCTAGG 3601 GGTCTTTCCC CTCTCGCCAA AGGAATGCAA GGTCTGTTGA ATGTCGTGAA GGAAGCAGTT 3661 CCTCTGGAAG CTTCTTGAAG ACAAACAACG TCTGTAGCGA CCCTTTGCAG GCAGCGGAAC 3721 CCCCCACCTG GCGACAGGTG CCTCTGCGGC CAAAAGCCAC GTGTATAAGA TACACCTGCA 3781 AAGGCGGCAC AACCCCAGTG CCACGTTGTG AGTTGGATAG TTGTGGAAAG AGTCAAATGG 3841 CTCTCCTCAA GCGTATTCAA CAAGGGGCTG AAGGATGCCC AGAAGGTACC CCATTGTATG 3901 GGATCTGATC TGGGGCCTCG GTGCACATGC TTTACATGTG TTTAGTCGAG GTTAAAAAAA 3961 CGTCTAGGCC CCCCGAACCA CGGGGACGTG GTTTTCCTTT GAAAAACACG ATGATAATAT 4021 GGCCACAACC ATGGCCAAGC CTTTGTCTCA AGAAGAATCC ACCCTCATTG AAAGAGCAAC 4081 GGCTACAATC AACAGCATCC CCATCTCTGA AGACTACAGC GTCGCCAGCG CAGCTCTCTC 4141 TAGCGACGGC CGCATCTTCA CTGGTGTCAA TGTATATCAT TTTACTGGGG GACCTTGTGC 4201 AGAACTCGTG GTGCTGGGCA CTGCTGCTGC TGCGGCAGCT GGCAACCTGA CTTGTATCGT 4261 CGCGATCGGA AATGAGAACA GGGGCATCTT GAGCCCCTGC GGACGGTGCC GACAGGTGCT 4321 TCTCGATCTG CATCCTGGGA TCAAAGCCAT AGTGAAGGAC AGTGATGGAC AGCCGACGGC 4381 AGTTGGGATT CGTGAATTGC TGCCCTCTGG TTATGTGTGG GAGGGCTAAC GCCCGCCCCA 4441 CGACCCGCAG CGCCCGACCG AAAGGAGCGC ACGACCCCAT GGCTCGACGA TAAAATAAAA 4501 GATTTTATTT AGTCTCCAGA AAAAGGGGGG AATGAAAGAC CCCACCTGTA GGTTTGGCAA 4561 GCTAGCTTAA GTAACGCCAT TTTGCAAGGC ATGGAAAAAT ACATAACTGA GAATAGAGAA 4621 GTTCAGATCA AGGTCAGGAA CAGATGGAAC AGCTGAATAT GGGCCAAACA GGATATCTGT 4681 GGTAAGCAGT TCCTGCCCCG GCTCAGGGCC AAGAACAGAT GGAACAGCTG AATATGGGCC 4741 AAACAGGATA TCTGTGGTAA GCAGTTCCTG CCCCGGCTCA GGGCCAAGAA CAGATGGTCC 4801 CCAGATGCGG TCCAGCCCTC AGCAGTTTCT AGAGAACCAT CAGATGTTTC CAGGGTGCCC 4861 CAAGGACCTG AAATGACCCT GTGCCTTATT TGAACTAACC AATCAGTTCG CTTCTCGCTT 4921 CTGTTCGCGC GCTTCTGCTC CCCGAGCTCA ATAAAAGAGC CCACAACCCC TCACTCGGGG 4981 CGCCAGTCCT CCGATTGACT GAGTCGCCCG GGTACCCGTG TATCCAATAA ACCCTCTTGC 5041 AGTTGCATCC GACTTGTGGT CTCGCTGTTC CTTGGGAGGG TCTCCTCTGA GTGATTGACT 5101 ACCCGTCAGC GGGGGTCTTT CACATGCAGC ATGTATCAAA ATTAATTTGG TTTTTTTTCT 5161 TAAGTATTTA CATTAAATGG CCATAGTTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG 5221 GCGGTTTGCG TATTGGGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG 5281 TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT 5341 CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA 5401 AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA 5461 ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC 5521 CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT 5581 CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA 5641 GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG 5701 ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT 5761 CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA 5821 CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT 5881 GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC 5941 AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA 6001 AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA 6061 ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT 6121 TGCGGCCGGC CGCAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA 6181 ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC 6241 CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC 6301 TGCAATGATA CCGCGAGACC CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC 6361 AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT 6421 TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT 6481 TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC 6541 CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG 6601 CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT 6661 TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC 6721 TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG 6781 CCCGGCGTCA ACACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT 6841 TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC 6901 GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC 6961 TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA 7021 ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG 7081 TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG 7141 CACATTTCCC CGAAAAGTGC CACCTGACGT CTAAGAAACC ATTATTATCA TGACATTAAC 7201 CTATAAAAAT AGGCGTATCA CGAGGCCCTT TCGTCTTCAC TCGAGGAGCT TGGCCCATTG 7261 CATACGTTGT ATCCATATCA TAATATGTAC ATTTATATTG GCTCATGTCC AACATTACCG 7321 CCATGTTGAC ATTGATTATT GACTAGTTAT TAATAGTAAT CAATTACGGG GTCATTAGTT 7381 CATAGCCCAT ATATGGAGTT CCGCGTTACA TAACTTACGG TAAATGGCCC GCCTGGCTGA 7441 CCGCCCAACG ACCCCCGCCC ATTGACGTCA ATAATGACGT ATGTTCCCAT AGTAACGCCA 7501 ATAGGGACTT TCCATTGACG TCAATGGGTG GAGTATTTAC GGTAAACTGC CCACTTGGCA 7561 GTACATCAAG TGTATCATAT GCCAAGTACG CCCCCTATTG ACGTCAATGA CGGTAAATGG 7621 CCCGCCTGGC ATTATGCCCA GTACATGACC TTATGGGACT TTCCTACTTG GCAGTACATC 7681 TACGTATTAG TCATCGCTAT TACCATGCTA GAGAACCATC AGATGTTTCC AGGGTGCCCC 7741 AAGGACCTGA AATGACCCTG TGCCTTATTT GAACTAACCA ATCAGTTCGC TTCTCGCTTC 7801 TGTTCGCGCG CTTCTGCTCC CCGAGCTCAA TAAAAGAGCC CACAACCCCT CACTCGGCGC 7861 GCCAGTCCTC CGATTGACTG AGTCGCCCGG GTACCCGTGT ATCCAATAAA CCCTCTTGCA 7921 GTTGCATCCG ACTTGTGG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.