pTac-GST-NUP98 plasmid

pTac-GST-NUP98 plasmid

PVT86084

pTac-GST-NUP98 plasmid Information

Plasmid name: pTac-GST-NUP98(human-toxic-opt)
Alias: NM_016320.5;ADIR2; NUP196; NUP96; Nup98-96
Gene lenght: 5403bp
Host: E.coli
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling:
Promoter: Tac
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone: pTac-GST-NUP98(human)
Forward primer: pGEX-5'
Reverse primer:
Copy number: Low
Induction: IPTG Induction

pTac-GST-NUP98 plasmid Description

pTac-GST-NUP98 This is a prokaryotic induction-type low-copy expression plasmid. The plasmid contains a Tac promoter that can initiate the transcription of the human NUP98 gene after codon optimization, promoting the N-terminal fusion expression of the NUP98 gene with a GST tag, which facilitates protein expression and purification. The plasmid carries LacI, LacO, and the Tac promoter, and can be induced by IPTG to regulate the expression level of the target gene.

pTac-GST-NUP98 plasmid Sequence

1 ACGTTATCGA CTGCACGGTG CACCAATGCT TCTGGCGTCA GGCAGCCATC GGAAGCTGTG 61 GTATGGCTGT GCAGGTCGTA AATCACTGCA TAATTCGTGT CGCTCAAGGC GCACTCCCGT 121 TCTGGATAAT GTTTTTTGCG CCGACATCAT AACGGTTCTG GCAAATATTC TGAAATGAGC 181 TGTTGACAAT TAATCATCGG CTCGTATAAT GTGTGGAATT GTGAGCGGAT AACAATTTCA 241 CACAGGAAAC AGTATTCATG TCCCCTATAC TAGGTTATTG GAAAATTAAG GGCCTTGTGC 301 AACCCACTCG ACTTCTTTTG GAATATCTTG AAGAAAAATA TGAAGAGCAT TTGTATGAGC 361 GCGATGAAGG TGATAAATGG CGAAACAAAA AGTTTGAATT GGGTTTGGAG TTTCCCAATC 421 TTCCTTATTA TATTGATGGT GATGTTAAAT TAACACAGTC TATGGCCATC ATACGTTATA 481 TAGCTGACAA GCACAACATG TTGGGTGGTT GTCCAAAAGA GCGTGCAGAG ATTTCAATGC 541 TTGAAGGAGC GGTTTTGGAT ATTAGATACG GTGTTTCGAG AATTGCATAT AGTAAAGACT 601 TTGAAACTCT CAAAGTTGAT TTTCTTAGCA AGCTACCTGA AATGCTGAAA ATGTTCGAAG 661 ATCGTTTATG TCATAAAACA TATTTAAATG GTGATCATGT AACCCATCCT GACTTCATGT 721 TGTATGACGC TCTTGATGTT GTTTTATACA TGGACCCAAT GTGCCTGGAT GCGTTCCCAA 781 AATTAGTTTG TTTTAAAAAA CGTATTGAAG CTATCCCACA AATTGATAAG TACTTGAAAT 841 CCAGCAAGTA TATAGCATGG CCTTTGCAGG GCTGGCAAGC CACGTTTGGT GGTGGCGACC 901 ATCCTCCAAA ATCGGATCTG GTTCCGCGTG GATCCCCGGA ATTCATGTTT AACAAATCCT 961 TCGGAACTCC ATTCGGCGGC GGCACAGGAG GTTTCGGAAC GACCAGCACA TTTGGACAGA 1021 ACACAGGCTT CGGAACCACA AGCGGCGGCG CATTTGGCAC CAGCGCATTC GGGTCAAGTA 1081 ATAACACAGG AGGGTTGTTT GGCAACAGTC AGACCAAGCC GGGAGGACTT TTTGGCACAA 1141 GTAGCTTCTC ACAGCCTGCC ACCAGTACTA GCACAGGTTT TGGCTTCGGG ACATCAACTG 1201 GGACAGCGAA CACACTGTTT GGGACCGCAT CCACCGGCAC AAGTCTCTTC AGCTCCCAGA 1261 ACAACGCCTT CGCCCAGAAC AAACCCACCG GCTTCGGCAA TTTCGGGACA AGCACGAGCT 1321 CAGGCGGACT CTTTGGGACT ACAAATACAA CCTCTAATCC CTTCGGGTCT ACAAGTGGCA 1381 GTTTGTTTGG CCCTAGCAGT TTCACCGCCG CTCCGACCGG AACGACTATC AAATTTAATC 1441 CCCCAACCGG GACCGATACA ATGGTAAAGG CGGGCGTGAG TACTAATATA TCTACGAAGC 1501 ACCAGTGCAT CACAGCCATG AAAGAGTACG AGAGCAAGAG TCTGGAGGAA CTCAGACTCG 1561 AGGACTATCA GGCCAATCGC AAAGGGCCCC AGAATCAGGT GGGAGCGGGG ACTACGACAG 1621 GCTTGTTCGG ATCATCCCCC GCTACTAGTT CAGCTACAGG CCTGTTCTCT TCCAGCACCA 1681 CAAATAGTGG ATTCGCCTAT GGCCAGAACA AAACGGCGTT TGGCACTTCA ACCACGGGGT 1741 TCGGAACAAA CCCAGGGGGT CTTTTTGGCC AGCAGAATCA GCAGACAACC AGTCTTTTCT 1801 CTAAGCCCTT CGGACAGGCT ACCACAACAC AGAATACCGG CTTCAGTTTC GGCAACACCA 1861 GCACAATCGG ACAACCCTCA ACCAACACGA TGGGACTGTT CGGTGTCACT CAGGCTTCTC 1921 AGCCTGGAGG ACTCTTTGGA ACAGCTACAA ATACTTCTAC TGGGACCGCT TTCGGTACCG 1981 GGACCGGGTT GTTCGGGCAA ACCAACACCG GATTTGGTGC CGTAGGCAGC ACTTTGTTTG 2041 GCAACAACAA GCTTACTACA TTTGGAAGCA GCACAACCAG TGCACCTTCA TTTGGTACAA 2101 CCAGTGGCGG GCTCTTTGGT TTTGGCACAA ATACCAGTGG GAATAGTATT TTTGGAAGTA 2161 AACCAGCACC TGGGACTCTT GGAACTGGGC TTGGTGCAGG ATTTGGAACA GCTCTTGGTG 2221 CTGGACAGGC ATCTTTGTTT GGGAACAACC AACCTAAGAT TGGAGGGCCT CTTGGTACAG 2281 GAGCCTTTGG GGCCCCTGGA TTTAATACTA CGACAGCCAC TTTGGGCTTT GGAGCCCCCC 2341 AGGCCCCAGT AGCTTTGACA GATCCAAATG CTTCTGCTGC CCAGCAGGCT GTTCTCCAGC 2401 AGCACATCAA TAGTCTAACA TACTCACCTT TTGGAGACTC TCCTCTCTTC CGGAATCCGA 2461 TGTCAGACCC TAAGAAGAAG GAAGAGAGAT TGAAACCAAC AAATCCAGCA GCCCAGAAGG 2521 CTCTTACTAC ACCTACTCAT TATAAACTGA CACCCCGCCC TGCCACTAGA GTCCGGCCAA 2581 AGGCTTTACA AACAACAGGC ACAGCCAAGT CACATCTCTT TGATGGGCTG GATGACGATG 2641 AACCATCCCT AGCCAATGGA GCATTCATGC CCAAGAAGAG CATTAAGAAG TTGGTTTTGA 2701 AGAACCTTAA TAATAGCAAT CTCTTTTCTC CTGTTAATCG TGATTCAGAA AATCTAGCTT 2761 CACCATCTGA ATATCCAGAA AATGGAGAGA GATTTAGTTT CCTAAGCAAA CCTGTTGATG 2821 AGAATCACCA GCAGGATGGA GATGAAGATT CCCTTGTTTC ACATTTTTAT ACTAACCCTA 2881 TTGCCAAACC TATTCCTCAA ACCCCAGAAA GTGCTGGAAA TAAACACAGC AACAGCAACA 2941 GTGTGGATGA TACCATTGTT GCATTAAACA TGCGTGCTGC TTTGCGAAAT GGGCTGGAAG 3001 GAAGCAGTGA AGAAACGTCT TTTCATGATG AGTCACTTCA GGATGACCGA GAAGAAATAG 3061 AAAATAATTC TTACCATATG CACCCAGCAG GTATTATTCT CACTAAGGTT GGTTACTATA 3121 CTATTCCATC TATGGATGAC CTTGCTAAAA TTACCAATGA AAAAGGAGAG TGCATTGTCT 3181 CTGATTTCAC TATTGGTCGG AAAGGTTATG GTTCAATCTA TTTTGAAGGA GATGTGAATT 3241 TGACAAATCT AAATTTGGAT GATATTGTGC ATATCCGGAG GAAAGAAGTA GTTGTCTACT 3301 TAGATGATAA CCAAAAACCA CCTGTGGGTG AAGGGCTAAA TAGGAAGGCT GAAGTTACAT 3361 TGGATGGAGT TTGGCCAACA GATAAAACAT CTCGTTGTTT AATAAAGAGC CCAGATCGCC 3421 TTGCTGATAT CAACTATGAA GGAAGATTGG AAGCAGTTTC AAGGAAACAG GGAGCTCAAT 3481 TCAAAGAATA CCGGCCTGAA ACTGGTTCTT GGGTGTTTAA GGTCTCCCAT TTTTCTAAGT 3541 ATGGCCTTCA GGATTCTGAT GAAGAGGAGG AGGAGCATCC GTCTAAAACT AGTACAAAGA 3601 AGTTGAAGAC TGCTCCTTTG CCTCCTGCAA GCCAGACTAC GCCCTTGCAG ATGGCTCTTA 3661 ATGGCAAACC TGCACCTCCA CCTCAGAGCC AGAGCCCAGA GGTGGAGCAG TTAGGGAGGG 3721 TTGTGGAACT GGACAGTGAC ATGGTAGATA TCACCCAGGA GCCAGTTTTG GATACCATGT 3781 TAGAAGAGAG CATGCCTGAG GATCAGGAAC CTGTGTCTGC CTCAACACAT ATTGCATCTT 3841 CACTGGGAAT TAATCCACAT GTCTTACAGA TCATGAAAGC ATCATTGCTT ACTGATGAAG 3901 AAGATGTAGA TATGGCACTG GATCAACGCT TCAGTCGCCT GCCTTCCAAA GCAGATACTT 3961 CTCAAGAAAT CTGTTCTCCC AGACTCCCCA TTTCAGCATC CCACTCGTCG AAAACTCGTT 4021 CACTAGTTGG TGGGTTACTA CAATCAAAAT TTACAAGTGG AGCTTTTCTT TCACCAAGTG 4081 TCTCTGTGCA AGAATGTCGT ACTCCCAGAG CAGCATCTTT AATGAATATC CCATCCACAT 4141 CCTCTTGGTC TGTCCCTCCA CCCCTGACTT CTGTGTTCAC AATGCCCAGC CCAGCCCCTG 4201 AGGTTCCGTT GAAAACAGTG GGTACACGTA GGCAACTAGG CCTAGTCCCT CGTGAAAAGT 4261 CTGTCACCTA TGGCAAGGGA AAACTCTTGA TGGACATGGC CCTATTCATG GGACGCTCAT 4321 TTCGTGTTGG TTGGGGCCCC AACTGGACTC TTGCTAATAG TGGAGAACAG CTGAATGGCT 4381 CTCATGAACT AGAAAATCAT CAGATTGCCG ATTCCATGGA GTTTGGATTC CTGCCCAATC 4441 CAGTAGCTGT TAAACCCCTA ACTGAGTCCC CATTCAAAGT TCACTTAGAA AAACTCAGTT 4501 TGAGACAAAG AAAGCCAGAT GAAGACATGA AATTATATCA GACACCTCTG GAGCTCAAAT 4561 TAAAACACAG CACTGTCCAT GTGGATGAAC TGTGTCCTCT CATTGTCCCC AATCTGGGAG 4621 TTGCTGTCAT TCATGACTAT GCAGATTGGG TTAAAGAAGC ATCAGGAGAC TTACCAGAAG 4681 CACAAATTGT GAAGCACTGG AGCCTGACAT GGACACTATG TGAAGCCCTA TGGGGCCACC 4741 TGAAGGAGCT TGACAGCCAG CTAAATGAAC CCCGTGAATA CATTCAAATT CTGGAGCGAA 4801 GAAGAGCTTT CTCCCGCTGG CTATCCTGTA CTGCCACACC TCAGATTGAA GAGGAAGTCT 4861 CCTTAACCCA AAAAAACAGC CCTGTGGAGG CTGTATTCAG CTACCTCACA GGCAAAAGGA 4921 TCAGTGAGGC CTGCTCTCTG GCCCAGCAGT CAGGGGATCA TCGTCTTGCT CTTCTTTTAT 4981 CTCAGTTTGT GGGTAGCCAG TCAGTCCGGG AGCTGCTCAC CATGCAGTTG GTGGACTGGC 5041 ATCAGCTCCA AGCAGACTCC TTCATCCAGG ATGAGAGACT GCGCATCTTT GCTCTGTTGG 5101 CTGGAAAACC GGTGTGGCAG CTCTCAGAAA AGAAGCAAAT AAACGTGTGC TCCCAGTTGG 5161 ATTGGAAACG CTCCCTGGCT ATCCATCTTT GGTATTTGCT TCCACCAACA GCCTCCATTT 5221 CTAGGGCGCT CAGCATGTAT GAAGAAGCAT TTCAGAATAC CTCTGACAGT GACAGATATG 5281 CCTGCTCCCC ACTTCCTTCG TATCTGGAGG GTTCTGGCTG TGTGATAGCG GAGGAGCAAA 5341 ACTCACAGAC ACCACTTCGA GATGTCTGCT TTCACCTTCT AAAACTCTAC AGTGACAGAC 5401 ATTATGATCT CAACCAGCTG CTGGAGCCTC GAAGCATAAC AGCAGATCCT TTGGACTACC 5461 GCCTAAGCTG GCACTTGTGG GAAGTGCTGA GGGCTCTTAA CTACACCCAT CTCTCAGCGC 5521 AGTGTGAAGG TGTGCTACAG GCCAGTTACG CTGGCCAGCT TGAAAGTGAG GGGCTCTGGG 5581 AGTGGGCCAT CTTTGTCCTC CTGCACATTG ACAACTCAGG CATACGTGAG AAGGCTGTTC 5641 GAGAGCTGCT TACCCGGCAC TGCCAGCTGT TGGAGACCCC TGAATCTTGG GCTAAAGAGA 5701 CTTTCCTTAC CCAGAAGCTC CGTGTACCTG CCAAATGGAT CCACGAGGCC AAAGCTGTGC 5761 GAGCACACAT GGAATCTGAC AAGCACTTAG AGGCCCTTTG CTTATTTAAG GCTGAGCACT 5821 GGAACCGCTG CCACAAGCTC ATCATCCGAC ACTTAGCTTC TGATGCCATC ATTAATGAGA 5881 ACTATGACTA CCTGAAGGGG TTCTTGGAAG ACCTGGCACC TCCAGAGCGC AGCAGCCTAA 5941 TTCAGGATTG GGAAACATCT GGGCTTGTTT ACCTGGACTA TATTAGAGTC ATTGAAATGC 6001 TCCGCCATAT ACAGCAGGTG GATTGCTCAG GTAATGACCT GGAGCAGTTA CACATCAAAG 6061 TGACTTCACT GTGCAGTCGG ATAGAGCAGA TTCAGTGTTA CAGTGCTAAA GATCGCCTGG 6121 CTCAGTCAGA CATGGCCAAA CGTGTAGCCA ACCTGCTGCG CGTGGTGCTG AGTCTGCATC 6181 ATCCTCCTGA TAGAACCTCC GACTCAACAC CAGACCCTCA GCGAGTCCCT TTGCGCCTCT 6241 TGGCTCCCCA CATTGGCCGG CTTCCCATGC CTGAGGACTA TGCCATGGAC GAACTGCGCA 6301 GCCTTACCCA GTCCTATCTG CGAGAACTGG CTGTTGGGAG CCTGTGACTC GAGCGGCCGC 6361 ATCGTGACTG ACTGACGATC TGCCTCGCGC GTTTCGGTGA TGACGGTGAA AACCTCTGAC 6421 ACATGCAGCT CCCGGAGACG GTCACAGCTT GTCTGTAAGC GGATGCCGGG AGCAGACAAG 6481 CCCGTCAGGG CGCGTCAGCG GGTGTTGGCG GGTGTCGGGG CGCAGCCATG ACCCAGTCAC 6541 GTAGCGATAG CGGAGTGTAT AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT 6601 TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA 6661 ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA 6721 TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC 6781 AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC 6841 ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT 6901 ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT 6961 TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG 7021 CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT 7081 CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG 7141 CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA 7201 AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG 7261 AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA 7321 TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC 7381 AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC 7441 CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA 7501 TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA 7561 GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA 7621 AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC 7681 ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC 7741 CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT 7801 CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC 7861 CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT 7921 TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT 7981 TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG 8041 CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA 8101 AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA 8161 CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG 8221 GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG 8281 AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC 8341 TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA 8401 ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG 8461 CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC 8521 GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA 8581 TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATAAAT TCCGACACCA 8641 TCGAATGGTG CAAAACCTTT CGCGGTATGG CATGATAGCG CCCGGAAGAG AGTCAATTCA 8701 GGGTGGTGAA TGTGAAACCA GTAACGTTAT ACGATGTCGC AGAGTATGCC GGTGTCTCTT 8761 ATCAGACCGT TTCCCGCGTG GTGAACCAGG CCAGCCACGT TTCTGCGAAA ACGCGGGAAA 8821 AAGTGGAAGC GGCGATGGCG GAGCTGAATT ACATTCCCAA CCGCGTGGCA CAACAACTGG 8881 CGGGCAAACA GTCGTTGCTG ATTGGCGTTG CCACCTCCAG TCTGGCCCTG CACGCGCCGT 8941 CGCAAATTGT CGCGGCGATT AAATCTCGCG CCGATCAACT GGGTGCCAGC GTGGTGGTGT 9001 CGATGGTAGA ACGAAGCGGC GTCGAAGCCT GTAAAGCGGC GGTGCACAAT CTTCTCGCGC 9061 AACGCGTCAG TGGGCTGATC ATTAACTATC CGCTGGATGA CCAGGATGCC ATTGCTGTGG 9121 AAGCTGCCTG CACTAATGTT CCGGCGTTAT TTCTTGATGT CTCTGACCAG ACACCCATCA 9181 ACAGTATTAT TTTCTCCCAT GAAGACGGTA CGCGACTGGG CGTGGAGCAT CTGGTCGCAT 9241 TGGGTCACCA GCAAATCGCG CTGTTAGCGG GCCCATTAAG TTCTGTCTCG GCGCGTCTGC 9301 GTCTGGCTGG CTGGCATAAA TATCTCACTC GCAATCAAAT TCAGCCGATA GCGGAACGGG 9361 AAGGCGACTG GAGTGCCATG TCCGGTTTTC AACAAACCAT GCAAATGCTG AATGAGGGCA 9421 TCGTTCCCAC TGCGATGCTG GTTGCCAACG ATCAGATGGC GCTGGGCGCA ATGCGCGCCA 9481 TTACCGAGTC CGGGCTGCGC GTTGGTGCGG ATATCTCGGT AGTGGGATAC GACGATACCG 9541 AAGACAGCTC ATGTTATATC CCGCCGTTAA CCACCATCAA ACAGGATTTT CGCCTGCTGG 9601 GGCAAACCAG CGTGGACCGC TTGCTGCAAC TCTCTCAGGG CCAGGCGGTG AAGGGCAATC 9661 AGCTGTTGCC CGTCTCACTG GTGAAAAGAA AAACCACCCT GGCGCCCAAT ACGCAAACCG 9721 CCTCTCCCCG CGCGTTGGCC GATTCATTAA TGCAGCTGGC ACGACAGGTT TCCCGACTGG 9781 AAAGCGGGCA GTGAGCGCAA CGCAATTAAT GTGAGTTAGC TCACTCATTA GGCACCCCAG 9841 GCTTTACACT TTATGCTTCC GGCTCGTATG TTGTGTGGAA TTGTGAGCGG ATAACAATTT 9901 CACACAGGAA ACAGCTATGA CCATGATTAC GGATTCACTG GCCGTCGTTT TACAACGTCG 9961 TGACTGGGAA AACCCTGGCG TTACCCAACT TAATCGCCTT GCAGCACATC CCCCTTTCGC 10021 CAGCTGGCGT AATAGCGAAG AGGCCCGCAC CGATCGCCCT TCCCAACAGT TGCGCAGCCT 10081 GAATGGCGAA TGGCGCTTTG CCTGGTTTCC GGCACCAGAA GCGGTGCCGG AAAGCTGGCT 10141 GGAGTGCGAT CTTCCTGAGG CCGATACTGT CGTCGTCCCC TCAAACTGGC AGATGCACGG 10201 TTACGATGCG CCCATCTACA CCAACGTAAC CTATCCCATT ACGGTCAATC CGCCGTTTGT 10261 TCCCACGGAG AATCCGACGG GTTGTTACTC GCTCACATTT AATGTTGATG AAAGCTGGCT 10321 ACAGGAAGGC CAGACGCGAA TTATTTTTGA TGGCGTTGGA ATT

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.