重组食蟹猴IL-12 Heterodimer蛋白

Recombinant Cynomolgus IL-12 Heterodimer Protein（产品说明）
蛋白名称：重组食蟹猴IL-12 Heterodimer蛋白
蛋白构建：A DNA sequence encoding the Cynomolgus IL12A (A0A2K5V4L8)(Arg57-Ser253) was expressed, constructed the plasmid 1 ; A DNA sequence encoding the Cynomolgus IL12B Heterodimer (G7P6S2)(Ile23-Ser328) was expressed, constructed the plasmid 2. The two plasmids were co-expressed and the Cynomolgus IL-12 Heterodimer was purified.
表达宿主：HEK293 Cells
蛋白纯度：≥ 85 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC. ≥ 90 % as determined by SEC-MALS(Routinely tested).
蛋白活性：1.Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is 0.9ng/mL (Routinely tested). 2.Immobilized Recombinant Cynomolgus IL-12 Heterodimer Protein(Cat: CT177-CNAH-UE) at 1 μg/mL (100 μL/well) can bind Anti-IL-12 & IL-23 (Research Grade Ustekinu*mab Biosimilar), the EC50 is 1.1 ng/mL (Routinely tested).
蛋白内毒素：< 0.5 EU per mg protein.
预测N端：Arg 57 & Ile 23
蛋白分子量：The recombinant Cynomolgus IL-12 Heterodimer comprises 503 (197+306) amino acids and has a calculated molecular mass of 57.32 (22.55+34.77) kDa. As a result of glycosylation, the bands migrate approximately 37-45 kDa and 70~kDa in the SDS-PAGE under reducing conditions. The molecular weight of this protein is around 67.7 kDa verified by SEC-MALS(Routinely tested).
蛋白NP号：A0A2K5V4L8 & G7P6S2
蛋白氨基酸序列：Arg57-Ser253 & Ile23-Ser328