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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃ to -80℃
- 保质期:
12个月
- 英文名:
Recombinant Mouse Tissue Factor Protein (His Tag)
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
1.00 mg/100.00 µg
| 规格: | 1.00 mg | 产品价格: | ¥42090.0 |
|---|---|---|---|
| 规格: | 100.00 µg | 产品价格: | ¥6470.0 |
蛋白名称:Mouse Tissue Factor Protein (His Tag), Endotoxin-Free
蛋白构建:A DNA sequence encoding the extracellular domain of mouse F3 (NP_034301.3) (Met 1-Glu 251) was expressed, with a C-terminal polyhistidine tag.
表达宿主:HEK293 Cells
蛋白纯度:≥ 90 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
蛋白内毒素:< 0.5 EU per mg protein.
预测N端:Ala 29
蛋白分子量:The recombinant Mouse F3 comprises 234 amino acids and has a predicted molecular mass of 26.8 kDa. As a result of glycosylation, the band migrates approximately 37-50 kDa in the SDS-PAGE under reducing conditions.
蛋白NP号:NP_034301.3
蛋白氨基酸序列:Met1-Glu251
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文献和实验His 标签蛋白纯化效果不理想,宝宝心里苦呀。今天,小编来跟大家一起找找原因。 纯化所得组分中没有收集到重组的His标签蛋白。该问题主要可以分为以下两个方面: 1、His标签蛋白没有结合到填料上就流穿了 原因一 超声的功率不对。超声功率过高,容易导致蛋白炭化;功率过低,蛋白释放不出来。 建议 改变超声功率,同时可以在超声前加入溶菌酶。 原因二 结合缓冲液条件不合适。 建议 检查结合缓冲液中是否有影响结合的因素,如:金属离子螯合剂、强还原剂、过高的咪唑浓度。同时,优化缓冲液的pH、盐
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