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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
Thalidomide 4'-ether-alkylC2-amine
- 库存:
999
- 供应商:
上海麦克林生化科技股份有限公司
- CAS号:
390367-50-7
- 规格:
100mg/250mg
| 规格: | 100mg | 产品价格: | ¥425.0 |
|---|---|---|---|
| 规格: | 250mg | 产品价格: | ¥790.0 |
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文献和实验The Thalidomide Disaster, Lessons from the Past
It is close to 60 years since thalidomide was created by the German company, Chemie-Gr�nenthal, and launched as “Contergan.” This was soon to be followed in England by the launch of “Distaval.” Of all the drugs developed in the intervening
Bases Buffer is Buffer C (as per Schroter)(as per SRF)pg.27 20mM Hepes pH 7.9 0.1% NP40 0.2mM EDTA 20% glycerol Lysis Buffer: 0.75M KCl Need buffers containing 0,0.1M,0.6M & 1.0M KCL in addition to 0.3M,1.5M & 2.0 used in SRF purification pg
分钟,再在95℃下孵育10分钟。 (10)测序反应体系 (a)1μl PCR product (b) 0.5μl BigDyeTM V1 (c) 0.75μl 5x dilution buffer (d) 0.25μl biotinylated M13 primer (10 pmoles/μl) (e) 2.5μl UHP H2O. 94℃ 2分钟,再(94℃ 30秒,50℃ 15秒,60℃ 4分钟)x 30个循环。然后按DYNAPURETM Dye Terminator
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