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文献和实验Amino-allyl Reverse Transcription
min. 1x dNTPS: 500uM each dATP, dCTP, dGTP, 200uM aadUTP, 300uM dTTP 50x recipe: FOR 2:3 10uL each 100mM dATP,dGTP,dCTP, 4uL 100mM aa-dUTP*, 6uL 100 mMdTTP * 5-(3-Aminoallyl)-2'-deoxyuridine 5'-triphosphate
Amino-allyl Reverse Transcription
Amino-allyl Reverse Transcription (this protocol was adapted by Joe DeRisi from one developed at Rosetta Inpharmatics) Resuspend the contents of one pack of the monofunctional NHS-ester Cy3 or Cy5 dye (mono-functional Cy3 or Cy
Preparation of probes for in situ hybridization
. The polylinker often contains GC rich stretches that induce cross-hybridization with ribosomal RNA (Witkiewicz H, Bolander ME, Edwards DR, 1993, Biotechniques vol 14, pp 458-463). III. Chemical conjugation of the amino-modified nucleic acids with activated
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