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- 文献和实验
- 技术资料
- 保存条件:
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
- 英文名:
Anti-GFP Antibody (3G86.32)
- 库存:
999
- 供应商:
abinscience
- 规格:
50ug/100ug/1mg
| 规格: | 50ug | 产品价格: | ¥1338.0 |
|---|---|---|---|
| 规格: | 100ug | 产品价格: | ¥2288.0 |
| 规格: | 1mg | 产品价格: | ¥9188.0 |

| Product name | Anti-GFP Antibody (3G86.32) |
|---|---|
| Catalog No. | ZP144083 |
| Form | Lyophilized |
| Storage buffer | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Purity | >90% as determined by SDS-PAGE. |
| Applications | ELISA |
| Endotoxin level | Please contact with the lab for this information. |
| Expression system | E. coli |
| Accession | \ |
| Protein length | \ |
| Nature | Recombinant |
| Predicted molecular weight | \ |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Alternative Names | \ |
| Species | \ |
| Note | For research use only. |
Abinscience, founded in 2023 and located in the innovation technology center in Strasbourg, France, is the core research reagent brand of ProteoGenix. Focusing on the development and production of life science research reagents, Abinscience takes "Empowering Bioscience Discovery" as its vision, and is committed to providing high-quality and innovative biological reagent products and technical solutions for global researchers.
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文献和实验Anti-GFP/BrdU In Situ Immunostaining
Materials 24 well dishes Millicell 0.4 µm inserts (Millipore PICM01250) - coated with fibronectin Culture medium PBS Fixative: 4% paraformaldehyde in PBS (pH 7.6) Rabbit anti-GFP (Molecular Probes, A-11122) FITC-goat anti-rabbit
Immunocytochemistry Protocol Using Anti-GFP Antibodies(使用抗GFP抗体的免疫细胞化学实验方案)
. 7. Wash the cells twice in PBS for 1 minute each with gentle agitation. 8. Prepare a 1:400 dilution of anti-GFP chicken antibody in PBS to obtain a final antibody concentration of 5.0 µg/mL. 9. Remove the PBS
【共享】如何检测GFP、GFP、YFP、EYFP、CFP抗体?
对照,2道为1:1000倍稀释我们先看一下EarthOx的单克隆GFP抗体——Anti-GFP Tag Monoclonal Antibody [GF28R](货号#E022030),其免疫原是KLH偶联的水母GFP蛋白的N末端多肽,由于EGFP、YFP、EYFP、CFP得N末端序列与GFP几乎完全一致,因此从免疫原上来说,GFP与EGFP、YFP、EYFP、CFP没有区别。更重要的是,EarthOx对于该单克隆抗体还进行了明确的验证试验,试验也表明该Anti-GFP Tag Monoclonal
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