- ￥9458.46
- Fitzgerald
- 美国
- 10R-I148AP
- 2026年03月09日
- ELISA, IF, IHC-F, IHC-P, IP
- Mouse
- human
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
Store at 4 deg C.
- 克隆性：
Monoclonal
- 适应物种：
human
- 宿主：
Mouse
- 应用范围：
ELISA, IF, IHC-F, IHC-P, IP
- 抗体英文名：
Involucrin antibody (BSA/Azide Free)
- 抗体名：
Monoclonal Involucrin antibody, Anti-Involucrin antibody
- 规格：
200 ug
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文献和实验Monoclonal Antibody Production Protocol
- PBS + 0.1% Tween 20 or TBS + 0.1% Tween 20. - Blocking solution = 2% BSA (type V ) in PBS. (Add 0.02% azide for longer storage.) - Elisa buffer = 2% BSA + 0.1% Tween 20 in PBS ( azide optional ). - Enzyme linked antibody = Horseradish peroxidase
step 5 min Antibody 15 min PBS Wash 6 x 2 min Protein A Gold 15 min PBS Wash 6 x 2 min Water Wash 5 x 1 min. Blocking Any "sticky" protein can be used to cover non-specific binding sites on the thawed sections
Purification of Endotoxin Free mAbs
pressure of circulation to force water out through the membrane, and rapidly concentrating the antibody/proteins up to 100 fold. The advantage of this method is very large volumes can be processed using sterile and endotoxin free components. Ammonium
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