- ￥5900
- GeneTex
- 美国
- GTX00811
- 2025年07月11日
- WB, ICC/IF, IHC-P, ELISA
- Rabbit
- Human, Mouse, Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
A synthesized peptide derived from human RUNX2 around the phosphorylation site of S28.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX00811
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, IHC-P, ELISA
- 浓度：
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
RUNX2 (phospho Ser28)
- 抗体英文名：
RUNX2 (phospho Ser28) antibody
- 抗体名：
RUNX2 (phospho Ser28) 抗体
- 规格：
100 μl
WB analysis of heat-shock treated HepG2 cell lysate using GTX00811 RUNX2 (phospho Ser28) antibody. The lane on the left was treated with blocking peptide.
ELISA analysis of phospho- and non phosho- immunogen peptide using GTX00811 RUNX2 (phospho Ser28) antibody.
P-peptide : phospho-peptide
N-peptide : non-phospho-peptide
Peptides concentration : 1μg/ml
IHC-P analysis of rat lung tissue using GTX00811 RUNX2 (phospho Ser28) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution : 1:100
IHC-P analysis of rat heart tissue using GTX00811 RUNX2 (phospho Ser28) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution : 1:100
IHC-P analysis of mouse heart tissue using GTX00811 RUNX2 (phospho Ser28) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution : 1:100
WB analysis of mouse thymus tissue lysate using GTX00811 RUNX2 (phospho Ser28) antibody pre-incubated with phospho- or non-phospho- peptide.
P-peptide : phospho-peptide
N-peptide : non-phospho-peptide
ICC/IF analysis of PFA-fixed HepG2 cells with LPS treatment for 4hrs using GTX00811 RUNX2 (phospho Ser28) antibody.
Red : Primary antibody
Green : Tubulin
Permeabilization : 0.1% Triton X-100
Dilution : 1:200
WB analysis of mouse thymus tissue lysate using GTX00811 RUNX2 (phospho Ser28) antibody pre-incubated with phospho- or non-phospho- peptide.
P-peptide : phospho-peptide
N-peptide : non-phospho-peptide
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文献和实验Using Phospho‐Motif Antibodies to Determine Kinase Substrates
comprising both the phosphorylated residue and the surrounding residues that determine kinase specificity, with degenerate residues taking up the remaining positions. Currently, several categories of phospho?motif antibody are commercially available
Optimized Protocol to Make Phospho-Specific Antibodies that Work
, not simply its level of expression. In this review, we will discuss both the design of the phosphopeptide immunogen and immunization. The affinity purification of the phospho-specific antibody as well as the methods most suitable for characterizing
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