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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
请参考说明书
- 亚型:
请参考说明书
- 形态:
液体
- 保存条件:
Aliquot and store at -20ºC
- 克隆性:
请参考说明书
- 标记物:
请参考说明书
- 适应物种:
human/mouse/rat等
- 宿主:
rabbit/mouse
- 应用范围:
ELISA,WB,IHC等
- 浓度:
请参考说明书
- 靶点:
请参考说明书
- 抗体英文名:
Cyclin D1 antibody [CY-D1]
- 抗体名:
Cyclin D1 antibody [CY-D1]
- 规格:
100μg
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文献和实验Immunodetection of cyclin D1 and D2/D3 using flow cytometry
that has been kept -20°C. Incubate the cells for at least two hours. Wash once with staining buffer and decant. Add the primary anti-cyclin antibody in a volume of 200 µ l. Incubate overnight at 4°C. Wash twice with cold cyclin staining buffer and add the Cy
Disorders of the cell cycle regulatory machinery play a key role in the pathogenesis of cancer. The gene encoding the cyclin D1 protein, a regulator of the progression from the G1- to S-phase, is often found disrupted in the cancer cell
Immunodetection of cyclin D1 and D2/D3 using 488/630 nm dual laser flow cytometr(图)
This protocol is for use with the D cyclins and employs 488 nm argon laser excitation of propidium iodide and 630 nm NeNe or diode laser excitation of the fluorochrome Cy5 to detect cell cycle-specific cyclin D expression
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