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文献和实验An ELISA Lab-on-a-Chip (ELISA-LOC)
Laminated object manufacturing (LOM) technology using polymer sheets is an easy and affordable method for rapid prototyping of Lab-on-a-Chip (LOC) systems. It has recently been used to fabricate a miniature 96 sample ELISA lab-on-a-chip
as antigen. 2. In a 96-well ELISA plate (Nunc MaxiSorp is best), add 100 ng of antigen in 50 µL in each well you will be using for your test, as well as four control wells. The perimeter wells on the plate are generally not used, as they tend to give poor
A.Sensitize a 96-well microtiter plate with purified antigen. 1.Prepare a solution of the purified antigen of interest in phosphate buffer (see recipe below) such that a concentration of approximately 10 µg/ml
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