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- 详细信息
- 文献和实验
- 技术资料
- 应用范围:
WB
- 适应物种:
Human,Mouse,Rat
- 标记物:
Unconjugated
- 保存条件:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- 形态:
Liquid
- 规格:
50μl/100μl
| 规格: | 50μl | 产品价格: | ¥1024.0 |
|---|---|---|---|
| 规格: | 100μl | 产品价格: | ¥1824.0 |
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文献和实验= NAL), appears to form upon oxidative cyclization of the nonfluorescent 2:1 lysine-HNE Michael adduct-Schiff base cross-link (Scheme 1). Polyclonal antibody (PAb) to the NAL-HNE fluorophore was raised in rabbit and found to be highly specific
Methods for use with the mTn-3xHA/GFP-mutagenized library
cross-reacting yeast proteins of about 55kD or110kD, respectively, and can give a spotty background on immunofluorescence. Despite this drawback, the 3xHA tag has been used extensively and successfully in yeast. A rabbit polyclonal antisera
-cre (selecting LEU2 ). Screening for in-frame lacZ fusions in yeast Transformant colonies are patched to SC-ura ( SC-ura -leu if you already have pB227/GAL-cre in there). Cells are replica plated to an SC-ura (-leu
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