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- 详细信息
- 文献和实验
- 技术资料
- 应用范围:
WB,IHC-F,IHC-P,FC
- 适应物种:
Human,Mouse,Rat
- 标记物:
Unconjugated
- 保存条件:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- 形态:
Liquid
- 规格:
50μl/100μl
| 规格: | 50μl | 产品价格: | ¥1024.0 |
|---|---|---|---|
| 规格: | 100μl | 产品价格: | ¥1824.0 |
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文献和实验ChIP protocol for X. laevis Lens1/FoxE3 enhancer
SDS lysis buffer/1x proteinase inhibitor cocktail (2.5 µl of 200 x stock from Upstate EZ ChIP kit). Roughly dissociate heads by pipetting, and transfer them into a 2 ml dounce homogenizer. Total volume is ~700 µl. �SDS lysis
Purification of Calmodulin from Bovine Brain
References: Watterson et al.(1980)J Biol Chem 255:962-75. Charbonneau et al.(1979)Biochem Biophys Res Commun 90:1039-47. Extraction of Calmodulin Solutions: 1.1 N NH4OH stored at room temperature. 2.1 N sulfuric acid in 50% AmSO4 stored at room
Immunofluorescence: Rabbit Anti-Murine RELMα
for 25 minutes. 3. Incubate the tissue section with blocking buffer for 20 minutes. 4. Incubate the tissue section overnight at 4?C with Rabbit Anti-Murine RELMα at 4.0 ng/mL in 1X PBS with 0.01% Triton-X and 0.5% BSA. Wash the slide
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