Aplo Shell 400

EM Fixation of Embryos

Agarose 5ul 1M MgCl2 400ul 0.2 M NaCacodylate (pH 7.4) 400ul sucrose (68.46g/100ml) 500ul 25% Glutaraldehyde Make up above mixture immediately before mounting embryos. The pad should be thick, so use triple thickness tape

Chick Chorioallantoic Membrane (CAM) Assay

CAM ASSAY Shell-less embryo culture Fertilized white leghorn chicken eggs (SPAFAS Inc., Norwich, CT) were received at day 0 and incubated for 3 days at 37°C with constant humidity. On day 3, eggs were rinsed with 70% ethanol and opened

Size and Shape of Protein Molecules at the Nanometer Level Determined by Sedimentation, Gel Filtration, and Electron Microscopy

% glycerol in 0.2 M ammonium bicarbonate and centrifuged in a Beckman SW55.1 swinging bucket rotor, 16 h, 38,000 rpm, 20°C. Twelve fractions of 400 μl each were collected from a hole in the bottom of the tube and each fraction was run on SDS-PAGE. Lane SM