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- 详细信息
- 文献和实验
- 技术资料
- 库存：
200
- 英文名：
NuGel-HemogloBind™ (Hemoglobin Removal) Kit
- CAS号：
N/a
- 保质期：
咨询
- 供应商：
南京北鱼生物科技有限公司
- 保存条件：
参考COA
- 规格：
kit
NuGel-HemogloBind™
Hemoglobin Removal with NuGel™ Dry-Bead Format
• Has a high degree of specificity for hemoglobin binding up to 10 mg/ml
• Species agnostic; including human, mouse, rat, sheep, bovine, goat, etc
• Removes hemoglobin from clarified organ/tissue lysates.
• Hemoglobin removal from red blood cell lysate for proteomics and biomarker drug discovery
• The flow through fractions (hemoglobin depleted) retain their enzymatic and biological activity
• Compatible with LC-MS, activity-based profiling and proteomic investigations
• Validated in the automation compatible high-throughput DPX Technologies XTR tip format
NuGel™ Hemoglobind™ is a modified version of suspension HemogloBind™, engineered for increased stability and improved installation for high-throughput formats. It is based on NuGel™ silica (50 microns in size, 1000Å) covalently bound to elastomeric polyelectrolytes. Comparable to selection properties of suspension HemogloBind™, at recommended quantities, >95% of hemoglobin can be removed with very high selectivity.
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文献和实验5
05142025
Selection of NuGel™HemogloBind ™ and related HemogloBind™ References
Leitner, Dominique F., et al. "Metabolomic, Proteomic, and Transcriptomic Changes in Adults with Epilepsy on Modified Atkins
Diet." Epilepsia (2023). For Plasma Metabolomics, the article states “Whole blood was thawed on ice and processed to remove
hemoglobin by NuGel-HemogloBind according to manufacturer protocol…”
Jara, Zaira Palomino, et al. "Distinct Mechanisms of β-Arrestin–Biased Agonist and Blocker of AT1R in Preventing Aortic Aneurysm
and Associated Mortality." Hypertension 80.2 (2023): 385-402. The article states “Cleaned abdominal aortas were homogenized in T-
PER Tissue Protein Extraction reagent…. Abdominal aneurysm samples were treated to remove hemoglobin captured within
the vessel wall. NuGel-HemogloBind … was used according to the manufacturer protocol.”
Zhang, X., Li, S., Malik, I. et al. Reprogramming tumour-associated macrophages to outcompete cancer
cells. Nature (2023). To measure the amino acid content by LC-MS from tumor
interstitial fluid, the article states “Samples were brought through a NuGel-HemogloBind prep prior to extraction to remedy
the levels of haemolysis present
E.Z.N.A. Endo-Free Plasmid Mini Kit II Spin Protocol
to completely neutralize the solution. Complete neutralization of the solution is vital of obtaining good yields. Endotoxin Removal using ETR Solution: 5. CAREFULLY transfer the cleared supernatant to a clean 1.5 ml centrifuge tube. Add
E.Z.N.A.® HP Total RNA Kit Spin Protocol Eukaryotic Cells and Tissues
实验原理 The E.Z.N.A.® HP Total RNA Kit uses the reversible binding properties of HiBind® matrix, a new silica-based material. This is combined with the speed of mini-column spin technology. A specifically formulated high salt
【转帖】2-D Clean-Up Kit 双向凝胶电泳--蛋白纯化
quality. I have used the kit for preparing samples from bacterial cells. The cells are lysed in a low salt buffer by sonication. I process the lysate using this kit to remove all the non-protein contaminants. The kit also helps in the removal
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