平末端/TA 连接酶预混液 | NEB

品牌	货号	产品中文名称	产品英文名称
NEB	M0367	平末端/TA 连接酶预混液	Blunt/TA Ligase Master Mix

货号	产品中文名称	产品英文名称	规格
M0367L	平末端/TA 连接酶预混液	Blunt/TA Ligase Master Mix	250 reactions
M0367S	平末端/TA 连接酶预混液	Blunt/TA Ligase Master Mix	50 reactions

产品特点

Blunt/TA Ligase Master Mix will ligate these substrates:

 

Blunt/TA Ligase Master Mix is a ready-to-use solution of T4 DNA Ligase, proprietary ligation enhancer, and optimized reaction buffer. This master mix is specifically formulated to improve ligation and transformation of both blunt-end and single-base overhang substrates.

• Simplifies reaction set-up, ensures an optimized ratio of enzyme and buffer components
• No thawing is necessary as it remains liquid during storage at -20°C
• Ligations for subcloning can be carried out in small volumes with low DNA concentrations, allowing users to conserve precious DNA samples and directly transform many strains of chemically competent E. coli without dilution
• Suitable for Adaptor Ligation
• Compatible with several Oxford Nanopore Technologies^® workflows, including some involving Covid-19 research
• Other T4 DNA Ligase products include Quick Ligation Kit, Salt-T4, Hi-T4 and Instant Sticky-end Ligase Master Mix
• Not sure which ligase to choose? Refer to our DNA and RNA Ligase Properties Chart or DNA Ligase Selection Chart

 

展开收起
Blunt/TA Ligase Master Mix is a ready-to-use solution of T4 DNA Ligase, proprietary ligation enhancer, and optimized reaction buffer. This master mix is specifically formulated to improve ligation and transformation of both blunt-end and single-base overhang substrates. The master mix format simplifies reaction set-up, ensures an optimized ratio of enzyme and buffer components, and yields robust, rapid ligation of all types of DNA ends using a short incubation time at room temperature. No thawing is necessary as it remains liquid during storage at -20°C.* Ligations for subcloning can be carried out in small volumes with low DNA concentrations, allowing users to conserve precious DNA samples and directly transform many strains of chemically competent E. coli without dilution.
Learn more about Joining Difficult to Ligase dsDNA Fragments and Efficient Adaptor Ligation for the Preparation of dsDNA Libraries using Blunt/TA Ligase Master Mix.
备注：以上仅为 M0367 的部分产品信息，如需NEB货号 M0367 的完整产品信息及相关的实验操作手册等等，请通过NEB官网查找对应的货号获取。