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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
KLH-conjugated synthetic peptide: DYKDDDDK (Sigma FLAG®)
- 形态:
Immunogen affinity purified serum, in PBS pH 7.4.
- 保存条件:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sid
- 克隆性:
Polyclonal
- 适应物种:
DYKDDDDK epitope tag (Sigma FLAG®), fused to proteins in plant cells
- 供应商:
Agrisera
- 宿主:
Rabbit
- 应用范围:
Western blot (WB)
- 靶点:
Anti-DYKDDDDK (binds to Sigma FLAG®) (polyclonal)
- 抗体英文名:
Anti-DYKDDDDK (binds to Sigma FLAG®) (polyclonal)
- 抗体名:
Anti-DYKDDDDK (binds to Sigma FLAG®) (polyclonal)
- 规格:
50 µg
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文献和实验Polyclonal and Monoclonal Antibodies
Polyclonal Antibody Immunization of Mice: Injections should be made at intervals of at least two weeks.. We have been using either of the two following adjuvants with success: Freund adjuvant and MPL+TDM adjuvant ( RIBI ImmunoChem
monoclonal anti-HA 16B12 (MMS101R, BAbCo, used at 1:200 to 1:1000 dilution) mouse monoclonal anti-HA 12CA5 (12CA5, Boehringer, used at 1:200 dilution) rabbit polyclonal anti-HA (RS1010C, BAbCo, used at 1:200 dilution) Cy3-conjugated affinity
Strep/FLAG Tandem Affinity Purification (SF‐TAP) to Study Protein Interactions
. no. 2‐1000‐025) 1:10 in H 2 O (final concentration, 2 mM desthiobiotin) Anti–FLAG M2 agarose (Sigma‐Aldrich) FLAG elution
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