- 询价
- Agrisera
- Sweden
- AS09 481-DL594
- 2026年03月02日
- Immunofluorescence (IF), Western blot (WB)
- Rabbit
- Arabidopsis thaliana, Brassica napus, Chlamydomonas reinhardtiiCucumis sativus, Nicotiana benthamiana, Raphanus sativa L, Tokinashi-daikon, Olea europaea, Picea abies, Physcomitrella patens, Spinacia oleracea, Solanum lycopersicum, Solanum tuberosum, Tri
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana BiP proteins: BiP1 At5g28540 Q9LKR3, BiP2 At5g42020 F4K007 , BiP3 At1g09080 Q8H1B3
- 形态：
Immunogen affinity purified serum, in PBS pH 7.4, conjugated to DyLight® 594.
- 保存条件：
Store at 4°C for 12-18 months, A preservative may be added for long time storage up to 2 years. Spin briefly the tube before use.
- 克隆性：
Polyclonal
- 适应物种：
Arabidopsis thaliana, Brassica napus, Chlamydomonas reinhardtiiCucumis sativus, Nicotiana benthamiana, Raphanus sativa L, Tokinashi-daikon, Olea europaea, Picea abies, Physcomitrella patens, Spinacia oleracea, Solanum lycopersicum, Solanum tuberosum, Tri
- 供应商：
Agrisera
- 宿主：
Rabbit
- 应用范围：
Immunofluorescence (IF), Western blot (WB)
- 靶点：
Anti-BiP | Lumenal-binding protein, DyLight® 594 conjugated (40 µg)
- 抗体英文名：
Anti-BiP | Lumenal-binding protein, DyLight® 594 conjugated (40 µg)
- 抗体名：
Anti-BiP | Lumenal-binding protein, DyLight® 594 conjugated (40 µg)
- 规格：
40 µg
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文献和实验Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
To immunostain using enzyme-coupled secondary antibodies: i. Rinse slides in PBS and remove excess solution. ii. Add 40 µL of secondary antibody (e.g., anti-rabbit IgG [Fc] HRP conjugate, 1:100 dilution
Dynabeads® Co-Immunoprecipitation Kit
from which the isolated protein complex is to be analyzed by Mass Spectrometry, elution in NH4OH based buffer (HPH EB) followed by drying in a centrifugal vacuum concentrator (e.g. SpeedVac®, ThermoSavant Inc.) is strongly recommended.
To fix, 1/10 volume of 37 formaldehyde is added to the culture, which is shaken for a further 40 minutes at room temperature. Cells are then recovered by centrifugation at 1400 X g for 2 minutes, and washed (i.e. gently resuspended then recovered
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