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文献和实验Clone Genes From a Phage Library
at 30 degrees, spin down in a 50 ml Falcon tube, and resuspend to half volume in 10 mM MgSO4 . These bacteria will be good to use for phage plating for several weeks.Use 50 microliters per plate. Filters: I like simple uncharged nylon filters from Fisher
Single Clone Excision Protocol
,so this selects for the transfected cells).If you want to do blue-white screening,spread 100 ml each of X-Gal (2% in dimethylformamide,stored in 15 ml conical tube in 4°)and IPTG (40mM in dH2O,stored same way)on the plate 30 min before plating.
Clone Genes From a Phage Library
which is the lambda receptor) overnight at 30 degrees, spin down in a 50 ml Falcon tube, and resuspend to half volume in 10 mM MgSO4. These bacteria will be good to use for phage plating for several weeks.Use 50 microliters per plate. Filters: I like simple uncharged
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