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文献和实验, although some hypotheseshave been suggested. The first hypothesis proposes lithium as amagnesium (Mg 2+ )-competitive inhibition of GSK-3 (16) , whereasthe second suggests lithium inhibitor by potassium deprivation (17) . Lithium is a nonspecific GSK-3 inhibitor
v468 Chapter 9 自身启动子报告基因验证转录靶点
of the reaction mix to electrophoresis on a 1%(w/v) agarose gel to check for correct size of the amplicon. 4. For subsequent purification of the amplicon from the reactionmix, use the Qiagen PCR Purification Kit according tothe user manual, and elute in 30 μL
DNA fingerprinting - agarose gel
stains in a foil covered container at 4℃. After staining, image gels using Fluorimager with the following scan settings: pixel size: 200 µm; digital resolution: 16 bits; detection sensitivity: high; PMT voltage: 950 V; filter :530 nm. Crop gel images
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