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- 详细信息
- 文献和实验
- 技术资料
- 库存:
10
- 供应商:
北京柏瑞图科技有限公司
- 规格:
100g
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文献和实验is ~300 micrograms RNA Materials Acid Phenol Heat new bottle to 65 deg (100g). Add 20 ml H2O + 0.2 g Hydroxyquinoline Let cool Add ~10 ml more H2O till a little water remains on top of phenol so that it is completely water saturated
Acid Phenol Yeast RNA Preparation
ml conical tube. Centrifuge 4 min @ 4 deg. Remove supernatant. Quickly resuspend pellet in 800 microliters cold TES and freeze in dry ice or liquid N2.3. Warm acid phenol in H2O bath to 65 deg.4. Thaw cells briefly on ice.5. Add 800 microliters warm
步骤。 siashq大侠通过给作者写信,最终确定步骤为: fix : 40 % ethanol + 10 % acetic acid 30min; wash: distilled water 15min * 4change; staining: (0.12% CBB G250 , 10% ammonium sulfate, 10% phosphoric acid and 20% methanol) overnight destain: distilled water 之后,czlong
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