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文献和实验Approaches to the Stable Transfection of G Protein-Coupled Receptors
receptor can be used to examine second messenger-couplmg pathways. In planning to establish a stably transfected cell line expressing a GPCR of interest, there are a number of different factors that must be taken into consideration.
as well. Additionally, we created a stable COS-7 cell line transfected with the pSilencer 3.1 H1 neo vector containing a selected siRNA template oligonucleotide (small hairpin interference RNA) where the levels of sortilin were greatly suppressed. The elimination
Inducible Expression of G Protein-Coupled Receptors in Transfected Cells
in the experimental model, or to induce an abrupt downregulation of receptor expression during the maintenance of the cell-line. This chapter provides an overview of the different systems available and provides methods for the generation and validation of stably
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