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文献和实验・ Lambda DNA Preparation (Stanford DNA Sequence & Technology Center) Detailed protocol for lambda DNA preparation with recipes ・ Isolation of Lambda DNA: Quick Method (Molecular Genetics Network Lab) ・
Purification of DNA from lambda phage
ml μltracentrifuged media. Alternative protocol for lambda DNA preparation: After step II A (RNase and DNase digestion)add 2.9 g NaCl and 5 g PEG-8000 to each 50 ml tube (be careful to exclude clumps of PEG-8000,which are hard to dissolve.)Shake
Preparation of Phage Lambda DNA
The isolation of phage DNA from a purified phage preparation simply involves the removal of the proteins of the phage particle. This is done most easily by phenol extraction (Method A). The large amounts of high quality DNA needed for use
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