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- 库存:
10
- 供应商:
北京柏瑞图科技有限公司
- 规格:
1 x 96 wells
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文献和实验selection parameters and integrates BLAST searches of the human and mouse genome databases. See http://jura.wi.mit.edu/pubint/http://iona.wi.mit.edu/siRNAext/ (registration required). Specific Guidelines for Designing siRNA Hairpins Encoded by siRNA
nM 5'biotin end-labeled siRNA under gentle agitation, i.e. a rocking plate (Figure 1a). We utilized the EF1 alpha specific siRNA, EF52, shown previously to direct TGS in human cells (Morris et al. 2004). The EF52 siRNA was constructed with a 5' biotin
against HIV-1 rev transcripts in human cells. Nature Biotechnology 20 : 500-505. 6. Yu, J.-Y., DeRuiter, S.L., and Turner, D.L. (2002) RNA interference by expression of short-interfering RNAs and hairpin RNAs in mammalian cells. Proc. Natl. Acad. Sci
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