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- 技术资料
- 库存:
10
- 供应商:
北京柏瑞图科技有限公司
- 规格:
1mg
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文献和实验complexes by pulse centrifugation (15 seconds at 3,000 RPM). Wash the bead/complexes 3 times in 500µl ice-cold TBS-mod buffer(comment 4); ;To elute the putative ChIP-Flag complexes (by competition with 3x Flag-peptide) add 100µl of TBS-mod buffer+3µl (5µg/µl
Peptide Pull-Down (PPD) Assay for Identification and Characterization of Histone PTM Effectors
histone PTM binding proteins can be used in conjunction with appropriately modified peptides (reviewed in Taverna et al. , 2007) II. Preparation of peptide-bound resin: Biotinylated peptides are conjugated to avidin beads to generate
seconds at 3,000 RPM). Wash the bead/complexes 3 times in 500µl ice-cold TBS-mod buffer (comment 4);To elute the putative ChIP-Flag complexes (by competition with 3x Flag-peptide) add 100µl of TBS-mod buffer+3µl (5µg/µl 3X Flag Peptide) to each pelleted
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