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- 2026年02月19日
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文献和实验A Cell-Detachment Solution Can Reduce Background Staining in the ELISPOT Assay
a background and then counted as specific spots. We have tested a cell detachment reagent, Accumax™, and found that it may be used to remove a large number of cells adhered to the microplate membranes. Accumax was tested in 16 different ELISPOT assays
Gold Nanoparticle Quantitation via Fluorescence in Solution and Cell Culture
This chapter provides a protocol for quantitative analysis of gold in solution as well as gold uptake by macrophages. A 96-well fluorescence assay was developed to be able to determine gold concentrations for a given gold nanoparticle
b-galactosidase Solution Assay
of 0.25 M Tris HCl pH 8.0. • Freeze thaw the cells 3 times by alternating between dry ice and 37°. • Spin for 15 minutes at 4° and transfer the supernatant to a new tube and hold on ice. • Mix the following reaction solution per sample: 264
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