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- 2026年05月28日
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0.12mg
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文献和实验Purification of Tubulin from Porcine Brain
-associated proteins require the availability of purified αβ-tubulin. In this chapter, we describe the process of purification of heterodimeric αβ-tubulin from porcine brain.
Isolation and Cultivation of Porcine Astrocytes
fragments, based on size and density. Astrocytes are further purified from any residual nonastrocytes by differential attachment during the first passage. The resulting culture is purely astrocytes (>98%) based upon their appearance in phase-contrast
Although many protocols for tubulin preparation are available, the procedure described below is the simplest and highest yielding preparation I have done. The protocol calls for 3 pig brains, and should yield ~ 60 mg of purified tubulin
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