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10
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北京柏瑞图科技有限公司
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100 uL
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文献和实验Aspects of CNS Lupus: Mouse Models of Anti-NMDA Receptor Antibody Mediated Reactivity
concepts of these models and provide protocols for the following: (1) the induction of anti-dsDNA, anti-NMDAR antibodies, (2) testing serum antibody titer by ELISA, (3) breaching blood brain barrier (BBB) integrity with LPS and epinephrine, (4) passive
Expression of Extracellular N-Terminal Domain of NMDA Receptor in Mammalian Cells
NMDA receptor is a ligand-gated ion channel receptor composed of multiple subunits encoded by at least five genes and their splice variants (1 ). Functional channels can be produced with only the NR1 subunit. These single-subunit channels
Protocol for anti-HA antibody Western Blotting
of interest. 2) Transfer proteins in acrylamide gel to nylon membrane. 3) Block overnight @4℃ in PBS (or TBS, it doesn't matter) with 5% non-fat dry milk. I dilute 2.5 mg in 50 ml. 4) Wash off excess milk sol'n with PBS x3 .(important if you are re-using your antibody
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