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文献和实验Transient transfection into 293T cel
. They (both) have the unusual property of being highly transfectable by the following Ca3 (PO4 )2 transfection protocol. Up to 50% efficiency is attainable.Materials• Complete medium: DMEM(high glucose) supplemented w/ 10% FCS (either w/ or w/o heat inactivation at 55°C/30min
Transient transfection into 293T cells
by the following Ca3 (PO4 )2 transfection protocol. Up to 50% efficiency is attainable. Materials • Complete medium: DMEM(high glucose) supplemented w/ 10% FCS (either w/ or w/o heat inactivation at 55°C/30min), NEAA, Na-pyruvate, and Gln (Pen/Streptmycin
Transient transfection into 293T cells
(either w/ or w/o heat inactivation at 55°C/30min), NEAA, Na-pyruvate, and Gln (Pen/Streptmycin: optional) • Transfection reagents: i) 2M CaCl2 : 2M in H2 O, filter sterile, store at 4°C ii) x2HBS: 8.0g NaCl, 0.37g KCl, 201mg (yes! mg) Na
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