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上海益启生物科技有限公司
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文献和实验Fast and reliable mini-prep RNA extraction from Neurospora crassa
, mycelia from plates (4 days old) and single colonies of N. crassa grown on a sorbose plate. With this protocol, from 20 to 300 mg (dry weight) of mycelium, grown in liquid media or on plates, yielded between 200 µg and 3 mg of RNA depending on the growth
Use of Flow Cytometric Methods to Quantify Protein‐Protein Interactions
Introduction Characterizing Protein‐Protein Binding Using the Flow Cytometry Protein‐Protein Interaction Assay Basic Protocol 1: Saturation Analysis of Biotin‐RGS with Alexa Fluor532‐Gαo
have been used to obtain enriched or purified cell populations, including flow cell sorting. Current protocols are usually time-consuming and may imply loss of short-lived RNAs, which is undesirable for expression profiling. We describe an optimized method
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