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文献和实验Backbone Amide Linker Strategy: Protocols for the Synthesis of C-Terminal Peptide Aldehydes
In the backbone amide linker (BAL) strategy, the peptide is anchored not at the C-terminus but through a backbone amide, which leaves the C-terminal available for various modifications. This is thus a very general strategy
Application of Amide Proton Exchange Mass Spectrometry for the Study of Protein‐Protein Interactions
describes amide proton exchange experiments that probe for changes in solvent accessibility at protein?protein interfaces. The simplest version of the protocol, termed the ?on?exchange? experiment, detects protein?protein interfaces by taking advantage
Objective, User-Independent ELISPOT Data Analysis Based on Scientifically Validated Principles
. In this way, the science of ELISPOT data analysis has been introduced, permitting exact frequency measurement against background. As ELISPOT assays become a gold standard for monitoring antigen-specific T-cell immunity in clinical trials, the need has surfaced
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