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文献和实验UV shadowing is a technique for visualizing nucleic acids separated on acrylamide/urea gels. The technique utilizes shortwave UV light (254 nm) and a fluor-coated TLC plate. We recommend UV shadowing as the method of choice for gel purification
Reprogramming Fibroblasts with the CytoTune-iPS Reprogramming Kit
4 Antibody 22. Rabbit Anti-SeV Antibody 23. Alexa Fluor® 488 Goat Anti-Mouse IgG (H L) Antibody 24. Alexa Fluor® 594 Goat Anti-Mouse IgG (H L) Antibody 25. Alexa Fluor® 488 Goat Anti-Rabbit IgG (H L) Antibody 26. Alexa Fluor® 594
-20 in 50 mM Na-phosphate buffer, pH 7.3. Goat-anti-mouse antibodies labeled with AlexaFluor-488 were from Invitrogen, Inc. (dye/protein ratio 7). Microscope glass slides, 3 × 1 in., and 1 mm thick, were from VWR. Water was purified by Milli-Q
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