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- 详细信息
- 文献和实验
- 技术资料
- 库存：
大量
- 英文名：
Trypan Blue, sodium salt *10 mM aqueous solution*CAS：72-57-1*
- CAS号：
72-57-1
- 保质期：
详见说明
- 供应商：
齐源生物
- 保存条件：
详见说明
- 规格：
10 mL
关键词：台盼蓝钠盐 10 mM水溶液*CAS：72-57-1*详细如下
Trypan blue, a diazo dye is a vital stain that colors dead tissues or cells blue. Live cells or tissues with intact cell membranes are not be colored. Since cells are very selective in the compounds that pass through the membrane, in a viable cell trypan blue is not absorbed; however, it traverses the membrane in a dead cell. Hence, dead cells are shown as a distinctive blue color under a microscope. Trypan blue is commonly used in microscopy (for cell counting) and in laboratory mice for assessment of tissue viability. The method cannot distinguish between necrotic and apoptotic cells. It is also useful to observe hyphea of fungi and Stramenopiles. We offer the highest purity of Trypan Blue.
齐源生物专业代理美国AAT Bioquest、其它产品详细如下：
| 货号 | 英文名称 | 规格 |
| AAT-104551S0 | PE/Texas Red® Anti-human CD45 Antibody *HI100* | 25tests |
| AAT-104551S1 | PE/Texas Red® Anti-human CD45 Antibody *HI100* | 100tests |
| AAT-104551S2 | PE/Texas Red® Anti-human CD45 Antibody *HI100* | 500tests |
| AAT-104551T0 | PerCP Anti-human CD45 Antibody *HI100* | 25tests |
| AAT-104551T1 | PerCP Anti-human CD45 Antibody *HI100* | 100tests |
| AAT-104551T2 | PerCP Anti-human CD45 Antibody *HI100* | 500tests |
| AAT-104551U0 | PerCP/Cy5.5 Anti-human CD45 Antibody *HI100* | 25tests |
| AAT-104551U1 | PerCP/Cy5.5 Anti-human CD45 Antibody *HI100* | 100tests |
| AAT-104551U2 | PerCP/Cy5.5 Anti-human CD45 Antibody *HI100* | 500tests |
| AAT-10456000 | Purified Anti-human CD45 Antibody *UCHL1* | 100ug |
| AAT-10456010 | iFluor™ 350 Anti-human CD45 Antibody *UCHL1* | 100tests |
| AAT-10456011 | iFluor™ 350 Anti-human CD45 Antibody *UCHL1* | 500tests |
| AAT-10456020 | iFluor™ 405 Anti-human CD45 Antibody *UCHL1* | 100tests |
| AAT-10456021 | iFluor™ 405 Anti-human CD45 Antibody *UCHL1* | 500tests |
| AAT-10456030 | iFluor™ 430 Anti-human CD45 Antibody *UCHL1* | 100tests |
| AAT-10456031 | iFluor™ 430 Anti-human CD45 Antibody *UCHL1* | 500tests |
| AAT-10456040 | iFluor™ 450 Anti-human CD45 Antibody *UCHL1* | 100tests |
| AAT-10456041 | iFluor™ 450 Anti-human CD45 Antibody *UCHL1* | 500tests |
| AAT-10456050 | iFluor™ 488 Anti-human CD45 Antibody *UCHL1* | 100tests |
| AAT-10456051 | iFluor™ 488 Anti-human CD45 Antibody *UCHL1* | 500tests |
| AAT-10456060 | iFluor™ 514 Anti-human CD45 Antibody *UCHL1* | 100tests |
| AAT-10456061 | iFluor™ 514 Anti-human CD45 Antibody *UCHL1* | 500tests |
| AAT-10456070 | iFluor™ 532 Anti-human CD45 Antibody *UCHL1* | 100tests |
| AAT-10456071 | iFluor™ 532 Anti-human CD45 Antibody *UCHL1* | 500tests |
| AAT-10456080 | iFluor™ 546 Anti-human CD45 Antibody *UCHL1* | 100tests |
| AAT-10456081 | iFluor™ 546 Anti-human CD45 Antibody *UCHL1* | 500tests |
| AAT-10456090 | iFluor™ 555 Anti-human CD45 Antibody *UCHL1* | 100tests |
| AAT-10456091 | iFluor™ 555 Anti-human CD45 Antibody *UCHL1* | 500tests |
| AAT-104560A0 | iFluor™ 560 Anti-human CD45 Antibody *UCHL1* | 100tests |
| AAT-104560A1 | iFluor™ 560 Anti-human CD45 Antibody *UCHL1* | 500tests |
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文献和实验问： 现在刚刚开始做干细胞，想自己计数白细胞，用的是Sigma的0.4%台盼蓝，1:1混合，从2分钟开始细胞死亡越来越多，例如2分时有10%死亡，到5分钟就死一半了，到底是几分钟计数最准确呢？& 还有一个问题，看到有1:1使用，也有1:9的，用哪种浓度最好呢？ 答1 ：0.4%应该是1:1混合的，1:9的是4%浓度的，这个染色的时间不应该太长的，因为对于胎盘蓝这种大分子，不能渗透就如细胞膜，但是如果染色
个/10mm2 时，说明稀释不当，需重制备细胞悬液、计数。 培养细胞活力的检测 台盼蓝排斥实验 1.制备单个细胞悬液，适当稀释； 2.向细胞悬液中加入0.4% 台盼蓝溶液，使台盼蓝终浓度为0.04% ； 3 ．在三分钟内用血球计数板分别计数活细胞和死细胞，死细胞被染成淡蓝色。 注意事项：在三分钟内完成死细胞计数，否则部分活细胞
直接计数细胞 1． 如果靶细胞是帖壁细胞，在细胞因子作用适当时间后，用生理盐水洗去死亡细胞，用 0.25 ％胰蛋白酶液消化细胞。加适量生理盐水吹打，使细胞分散成单个细胞。直接在血细胞计数板上计数细胞。 2． 如果靶细胞是悬浮细胞，则需要用染色剂区分死细胞和活细胞然后再计数。通常用台盼蓝染色细胞，活细胞可以排除进入细胞的台盼蓝而不着染，死细胞着染呈深蓝色。 3． 计数血细胞计数板上 4 个大方格中的全部活细胞，按下式
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