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WesternBright ECL发光液

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  • 询价
  • Advansta已认证
  • WesternBright ECL
  • 美国
  • 2026年05月02日
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    1金牌会员
  • 企业认证

    • 详细信息
    • 文献和实验
    • 技术资料
    • 库存

    • 英文名

      WesternBright ECL

    • 供应商

      环亚生物科技有限公司

    WesternBright ECL发光液

    WesternBright ECL灵敏度高,可以缩短曝光时间,减少上样量和抗体使用量。
     

    美国Advansta公司成立于2005年,总部位于美国加利福尼亚,是专业的生命科学试剂制造商,致力于研发高效易用的蛋白质分析试剂。其旗舰产品,WesternBright化学发光液产品线被评为市场上最灵敏的化学发光底物。
     

    WesternBright ECL发光液的优势

    • 信号强度高于Amersham™ ECL 和 Pierce™ ECL

    • 低皮克级灵敏度

    • 抗体浓度仅需常规用量的1/10

     

    应用方向

    • Western Blotting

     

    用户评价

    "After trying WesternBright ECL, we immediately switched from our usual brand and now use it on a weekly basis. It produces a strong, long-lasting signal with low background at a much lower cost."
    -Assistant Professor, Brigham Young University
     
    "WesternBright ECL is better than (the ECL substrate) I used to use because of its high sensitivity. Most of my research involves detecting low amounts of induced proteins, such as gp91 phox and HIF-1. I had some challenge to detect these proteins when I used other substrates; however, WesternBright ECL helps to detect these targets. Now, I can detect these proteins using less sample with the WesternBright ECL system. My whole lab has now switched to WesternBright ECL."
    -Senior Research Lab Specialist, University of Michigan
     
    "We have switched to the Advansta WesternBright ECL reagent for straight western and co-IP western blotting. We have used less antibodies to suit our various needs easily. By the way, the WesternBright reagent also worked beautifully during a recent demo of a competitor's chemiluminescent imager! It performed better than the vendor's own proprietary reagent made especially for that machine!" 
    -Lab Manager, University of North Carolina at Chapel Hill

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    图标文献和实验
    相关实验
    • ECL Western

      (All steps with agitation, unless done O/N at 4℃)►Rinse membrane once in TBS-T►Block the membrane with TBS-T/ 5% milk (or HIHS) for 30 min room►temp (can be left at 4℃ O/N here)►Rinse membrane briefly 2X with TBS-T and then once for 10 min with TBS

    • Western blotting using ECL reagent

      REAGENTSECL Western blotting kit (Amersham Life Science; cat# RPN2108): contains second antibodies for both mouse and rabbit, substrate and milk blocker (the milk blocker is not normally used when using ruminant samples).Hybond ECL nitrocellulose

    • 液相核酸分子发光液相杂交

      1 能量传递法:Heller等设计用两个紧接的探针,一个探针的一端用化学发光基团(供体)标记,另一个探针的一端用荧光物质标记,并且这两个探针靠得很近。两个靠得很近的探针用不同的物质标记(标记光发射),当探针与特异的靶杂交后,这些标记物靠得很近。一种标记物发射的光被另一种标记物吸收,并重新发出不同波长的光,调节 检测器使自动记录第二次发射光的波长。只有在两个探针分子靠得近时,才能产生受激发光,因此这种方法具有较好的特异性。  2 吖啶翁酯标记法:吖啶翁酯标记探针与靶核酸杂交后,未杂交

    图标技术资料

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