相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验E.Z.N.A. Cycle-Pure Kit Spin Protocol
of the sample at 260nm and then at 280nm. The DNA concentration is calculated as follows: 260 DNA concentration = A x 50 x (Dilution Factor) ug/ml Fragments greater than 500bp in length can routinely be purified at > 80% yield. 260 Bands ranging
。1.4.2 荧光标记差异显示PCR 选取与逆转录引物序列相同的带荧光物 质标记的锚定引物[TMR-T7(dT12)AP],随机引物(5'ACAATTTCACACAGGAACGCTAGTTG 3'),以逆转录产物为模板,进行PCR反应。反应体系包括:20 mmol/L Tris-HCl(pH8.4),50 mmol/L KCl,3.75 mmol/L MgCl2,逆转录产物3.0 μl,50 μ mol/L dNTP mi x(1∶1∶1),0.35 μmol/L 5'-随机引物,0.35 μmol/L
培养细胞RNA提取--RNA Isolation Protocol-Cultured Cell Samples
not to disturb the pellet. Allow the pellet to air dry for 10-15 minutes. Do not allow the pellet to completely dry out. Resuspend the pellet in 200-400ul of Ultra-pure water. Spec the RNA at OD 260 to determine the ug amount of RNA. Add appropriate
技术资料暂无技术资料 索取技术资料





