6-Well Ultra-Low Adherent Plat

Transduction Protocol for Adherent Cell Types

as cross-linkers at 4mg/ml dissolved in Milli-Q water and 0.22μm Filter Sterilized prior to use. Procedure 24 hours prior to transduction: - Split target cells into a 6-well tissue culture plate at 2.0-3.0x10 5

Determining Relative Transduction of Adherent Cells

of transfection) 实验步骤     1. Day 0   1)   Count cells and plate in a tissue culture plate such that 24 hours later cells

24h Subcloning Protocol

on ice for 2 min. 22.  Add 900 μl of LB and incubate at 37 ℃ with shaking for 30 min. 23.  Spin tube at 5K rpm for 2 min.  Remove all but 100 μl of the LB and resuspend pellet. 24.  Plate pellet on LB plate with appropriate antibiotic. 25.Incubate