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文献和实验Gene Editing in One‐Cell Embryos by Zinc‐Finger and TAL Nucleases
Wiley & Sons, Inc. Keywords: pronucleus injection; gene targeting; mouse mutant; zinc?finger nuclease; TAL nuclease; homologous recombination
4. Vortex vigorously. 5. Incubate at 100 deg C for 3 minutes. 6. Transfer immediately to 58 deg C and incubate overnight. S1 digestion buffer (final concentration): 300 mM NaCl 30 mM sodium acetate, pH 5.5 2 mM zinc sulfate 1000 units/ml S1 nuclease
immediately to 58 deg C and incubate overnight.S1 digestion buffer (final concentration):300 mM NaCl30 mM sodium acetate, pH 5.52 mM zinc sulfate1000 units/ml S1 nucleaseFor 1 ml of digestion buffer:60 µl 5M NaCl10 µl 3M sodium acetate, pH 5.520 µl 0.1M zinc
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