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- 详细信息
- 文献和实验
- 技术资料
- 库存:
大量
- 英文名:
Recombinant Nipah vRecombinant Nipah virus/HeV F/Fusion glycoprotein F0 Protein, C-His
- 保质期:
6-12months
- 供应商:
上海泽叶生物科技有限公司
- 保存条件:
-20℃
- 规格:
50µg/200ug/1mg
| 规格: | 50µg | 产品价格: | ¥1788.0 |
|---|---|---|---|
| 规格: | 200ug | 产品价格: | ¥2868.0 |
| 规格: | 1mg | 产品价格: | ¥17268.0 |
Recombinant Nipah virus/HeV F/Fusion glycoprotein F0 Protein, C-His
货号:ZY60011PP
品牌:泽叶生物
描述:
Recombinant Nipah virus/HeV F/Fusion glycoprotein F0 Protein, C-Hisexpressed in Mammalian Cells, spanning Ile27-Ser493.
Purity: >90% by SDS-PAGE.
HIGHLIGHTS:
His-Tagged — N-terminal 6×His tag for IMAC purification.
Mammalian Expression — Proper folding and native glycosylation.
High Purity — >90% purity verified by SDS-PAGE.
表达系统 Mammalian Cells
Accession号:Q9IH63
蛋白长度:Ile27-Ser493
应用:ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属:Nipah virus(HeV)
性质:Recombinant
内毒素水平:Please contact with the lab for this information.
纯度:>90% as determined by SDS-PAGE.
预测分子量: 57.71 kDa
状态:Lyophilized
保存溶液:Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬: Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL:
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
运输: In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名:Fusion glycoprotein F0, Protein F, Fusion glycoprotein F2, Fusion glycoprotein F1, F
Note:For research use only.
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文献和实验Sequences derived from the respiratory syncytial virus (RSV) fusion (F) protein were expressed in insect cells as recombinant glutathione-S -transferase (GST)-tagged proteins. The sequence covering the F2 subunit (GST-F2), and a truncated
【分享】两本糖生物学方面的 HUMANA Protocols
of the respiratory syncytial virus fusion protein. Also covered in this volume are uses of monoclonal antibodies and lectins, the spike glycoprotein, secretion of respiratory syncytial vius F protein from insect cells, the expression and characterization
Strategies to Optimize Protein Expression in E. coli
Peng, L., Xu, Z.N., Fang, X.M., Wang, F., Yang, S., and Cen, P.L. 2004. Preferential codons enhancing the expression level of human beta‐defensin‐2 in recombinant Escherichia coli. Protein Pept. Lett. 11:339
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