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- Cell Signaling Technology
- USA
- 6495S
- 2026年02月18日
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- 保存条件：
-20℃
- 供应商：
赛信通（上海）生物试剂有限公司
- 规格：
300µl
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文献和实验the anti-Flag-M2 beads. Response: This has been changed accordingly and the saved input is now in step 14 of Part-II. 5.For preparation of genomic DNA by Qiagen DNeasy kit, guanidine hydrochloride would be used at the lysis step. Can DNA (or RNA)-siRNA
and can be found in the very useful siRNA users guide.ii. Calcium phosphate Day 1 Plate HeLa cells at 75000 cells/ml in DMEM with 10% FCS. Day 2 Transfect in late afternoon/evening. Mix siRNA with filter-sterilised 0.25 M CaCl2 then with filter-sterilised BBS (50 mm BES, pH 6.95
实验原理 The isolation protocol relies on base pairing between the polyA residues at the 3’ end of most mRNA, and the oligo (dT)25 residues covalently coupled to the surface of the Dynabeads® . Other RNA species lacking a polyA
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