Recombinant Cynomolgus CD3D&CD

Recombinant Cynomolgus CD3D&CD3E Heterodimer (C-Fc-Flag&C-Fc-6His) (C09K)

产品说明(Description)

Recombinant Cynomolgus monkey CD3D&CD3E Heterodimer is produced by our Mammalian expression system and the target gene encoding Phe22-Ala105&Gln22-Asp117 is expressed with a Fc-Flag, Fc-6His tag at the C-terminus.

Accession #: Q95LI8&Q95LI5

Known as: CD3E & CD3D; CD3 delta & CD3 epsilon; CD3 delta / epsilon

 

制剂(Formulation)

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

 

质量控制(Quality Control)

Purity:        Greater than 95% as determined by reducing SDS-PAGE.    

Endotoxin: Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.

 

复溶(Reconstitution)

Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.    

 

保存(Storage)

Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.

Reconstituted protein solution can be stored at 4-7°C for 2-7 days.

Aliquots of reconstituted samples are stable at < -20°C for 3 months.     

 

背景(Background)

T-cell surface glycoprotein CD3D & CD3E, also known as CD3 delta & CD3 epsilon chain, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon(CD3E) , CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.

 

电泳(SDS-PAGE)

 

FOR RESEARCH USE ONLY