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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
蛋白表达区间:Phe22-Ala105&Gln22-Asp117
- 保质期:
See instructions
- 英文名:
标签:C-Fc-Flag&C-Fc-6His
- 库存:
表达系统:Human Cells
- 供应商:
上海经科化学科技有限公司
- 规格:
10ug/50ug/500ug/1mg
| 规格: | 10ug | 产品价格: | ¥570.0 |
|---|---|---|---|
| 规格: | 50ug | 产品价格: | ¥1700.0 |
| 规格: | 500ug | 产品价格: | ¥11900.0 |
| 规格: | 1mg | 产品价格: | ¥17800.0 |
- Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
- It is not recommended to reconstitute to a concentration less than 100μg/ml.
- Dissolve the lyophilized protein in distilled water.
- Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
- The product is shipped at ambient temperature.
- Upon receipt, store it immediately at the temperature listed below.
- Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.
- Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
- Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
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文献和实验T-cell surface glycoprotein CD3D & CD3E, also known as CD3 delta & CD3 epsilon chain, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon(CD3E) , CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.
作为 一种理想的分析特异性抗体的格式被人们所重视 [13] 。为了在单个蛋白质芯片上分析几 种不同的抗体,可能需要运用不同的方法 [ 24,25 ] 。与 Western 印迹相比,运用蛋白质芯片分析抗体有一些优点 [13],其中包括高灵敏度的蛋白质检测 [13,23] 。 蛋白质组学方法,如 2D 电泳/质谱(2-DE/MS ) 方法或是蛋白质芯片技术,在植物学领域中的应用越来越多 [ 26~32 ]。在此,我们介绍一种构建植物蛋白质芯片以及利用蛋白质芯片研究抗原抗体相互作用的方法。我们已成
., Krawczak, M., Cooper, D.N., and Chuzhanova, N.A. 2005. Microdeletions and microinsertions causing human genetic disease: Common mechanisms of mutagenesis and the role of local DNA sequence complexity. Hum. Mutat. 26:205‐213
X-100或脱氧胆酸钠)和低浓度变性剂(2mol/L尿素或盐酸胍等)洗涤除去脂类和膜蛋白,这一步很重要,否则会导致包涵体溶解和复性的过程中重组蛋白质的降解[6、7、8]。 包涵体的溶解必须用很强的变性剂,如8mol/L尿素、6~8mol/L盐酸胍,通过离子间的相互作用破坏包涵体蛋白间的氢键而增溶蛋白。其中尿素的增溶效果稍差,异氰盐酸胍最强;去污剂,如SDS[7],可以破坏蛋白内的疏水键,可以增溶几乎所有的蛋白,但由于无法彻底去除而不允许用在制药行业中;酸,如70%甲酸[9],可以破坏
技术资料







